We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Papers
20 resultsShowing papers similar to Targeted aggregation of PETase towards surface of Stenotrophomonas pavanii for degradation of PET microplastics
ClearDegradation of PET Plastics by Wastewater Bacteria Engineered via Conjugation
Researchers demonstrated a proof-of-concept approach for reducing PET microplastic pollution in wastewater by engineering bacteria in situ via conjugation to express PET-degrading enzymes. The study used a broad-host-range conjugative plasmid to transfer PET hydrolase genes into native wastewater bacterial communities.
Enzymatic Degradation of Polyethylene Terephthalate Plastics by Bacterial Curli Display PETase
Researchers engineered bacteria to display a PET-degrading enzyme on their surface, creating a reusable biocatalyst capable of breaking down polyethylene terephthalate plastics. The system worked under various conditions, remained stable for at least 30 days, and could even degrade PET microplastics in wastewater and highly crystalline consumer plastic waste. This biological approach offers a promising environmentally friendly alternative for plastic recycling and waste treatment.
Enhanced degradation of polyethylene terephthalate (PET) microplastics by an engineered Stenotrophomonas pavanii in the presence of biofilm
Scientists engineered a biofilm-forming bacterium to break down PET microplastics (the type found in water bottles and food containers) at room temperature. The engineered bacteria achieved significant PET degradation over 30 days and also worked on other polyester plastics, offering a potential biological solution for cleaning up microplastic pollution in water environments.
Display of PETase on the cell surface of Escherichia coli using the anchor protein PgsA
This study engineered bacteria to display a PET-degrading enzyme (PETase) on their cell surface, eliminating the costly step of purifying the enzyme for plastic breakdown. The approach could reduce the cost of biological PET plastic recycling, potentially offering a more scalable pathway for breaking down one of the most common plastic types.
Development and characterization of a bacterial enzyme cascade reaction system for efficient and stable PET degradation
Scientists engineered a bacterial system that displays plastic-degrading enzymes on the cell surface to efficiently break down PET plastic, achieving a 23% degradation rate of microplastics within 7 days. The system uses E. coli bacteria with specially designed protein fibers that both grip and digest PET fragments. This biotechnology approach could eventually help address the growing problem of microplastic pollution in water and soil environments.
Silica immobilized PETase for microplastic bioremediation: Influence of linker peptides on activity
Researchers immobilized a modified PETase enzyme onto silica using different linker peptides and tested its ability to break down PET microplastics, finding that linker peptide design significantly influenced enzyme activity and reusability — key parameters for practical application in wastewater treatment.
Eco-Microbiology: Discovering Biochemical Enhancers of PET Biodegradation by Piscinibacter sakaiensis
This paper reviews biochemical strategies for enhancing PET biodegradation by microorganisms, focusing on the discovery and engineering of plastic-degrading enzymes. The review highlights recent advances and remaining challenges in scaling up enzymatic plastic degradation for industrial applications.
Improvement of biodegradation of PET microplastics with whole-cell biocatalyst by interface activation reinforcement
Researchers developed a whole-cell biocatalysis strategy using alkali-resistant bacteria combined with surfactant-mediated interfacial activation to improve the biodegradation of PET microplastics, finding that Tween 20 most effectively enhanced the bio-interfacial activity between bacterial enzymes and the hydrophobic PET surface, leading to improved hydrolysis rates.
Interfacial engineering-based colonization of biofilms on polyethylene terephthalate (PET) surfaces: Implications for whole-cell biodegradation of microplastics
This study applied interfacial engineering to promote biofilm colonization on polyethylene terephthalate (PET) surfaces to facilitate enzymatic depolymerization under mild conditions. The engineered biofilm approach enabled efficient PET biodegradation without requiring harsh alkaline conditions or high temperatures, advancing practical plastic bioremediation.
Biodegradation of highly crystallized poly(ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin
Researchers engineered yeast cells to display both a PET-degrading enzyme (PETase) and a sticky protein (hydrophobin) on their surface simultaneously, dramatically improving the breakdown of highly crystalline PET plastic — achieving a 329-fold increase in degradation rate compared to the purified enzyme alone. This whole-cell biocatalyst approach could make enzymatic plastic recycling far more practical and efficient.
Modulating biofilm can potentiate activity of novel plastic-degrading enzymes
Researchers discovered two new enzymes capable of breaking down PET plastic (the kind used in plastic bottles) and found that boosting a bacterium's ability to form a biofilm — a sticky coating that helps bacteria cling to surfaces — significantly increased how fast the enzymes could degrade plastic. This biofilm strategy could help accelerate the development of biological plastic-recycling systems for waste that would otherwise end up in landfills.
An efficient strategy to tailor PET hydrolase: Simple preparation with high yield and enhanced hydrolysis to micro-nano plastics
This study developed a simplified, high-yield preparation method for PET-degrading hydrolase enzymes to improve their ability to break down PET nano- and microplastics. The engineered enzyme showed enhanced hydrolysis activity against PET microplastics, offering a more practical route to enzymatic plastic waste treatment.
Biodegradation of PET by the membrane-anchored PET esterase from the marine bacterium Rhodococcus pyridinivorans P23
Researchers identified a membrane-anchored enzyme from the marine bacterium Rhodococcus pyridinivorans that can break down PET plastic. The enzyme, displayed on the cell's surface, not only depolymerizes PET but also hydrolyzes its breakdown products under acidic conditions. The study provides new insight into how marine microorganisms naturally biodegrade plastic pollution, which could inform future bioremediation strategies.
Degradation of polyethylene terephthalate (PET) plastics by wastewater bacteria engineered via conjugation
Scientists engineered wastewater bacteria to break down PET plastic, one of the most common microplastic types, by transferring plastic-degrading genes through a natural DNA-sharing process. The modified bacteria could partially degrade a consumer PET product in 5 to 7 days. This proof-of-concept approach could help reduce the amount of microplastics released from wastewater treatment plants into the environment.
Engineered Vibrio natriegens as a living biocatalyst for in-situ biodegradation of microplastics in seawater
Researchers engineered the fast-growing marine bacterium Vibrio natriegens to display PETase enzymes on its outer membrane, creating a living biocatalyst that degrades PET microplastics directly in seawater conditions, outperforming comparable E. coli-based systems in both growth rate and hydrolytic activity. This halophilic whole-cell approach addresses a key gap in bioremediation — most PETase studies use freshwater organisms that cannot survive the salinity of marine environments where plastic pollution is most severe.
Microbial Degradation of (Micro)plastics: Mechanisms, Enhancements, and Future Directions
This review examines how microorganisms can break down microplastics using enzymes like PETase and laccases, offering a more environmentally friendly alternative to other cleanup methods. While microbial degradation holds promise for reducing microplastic pollution and its associated health risks, current efficiency is too low for large-scale application and needs further improvement.
Enhancing PET Degrading Enzymes: A Combinatory Approach
Scientists worked on improving enzymes that can break down PET plastic, one of the most common plastics in consumer products. Using a combinatory approach, researchers enhanced the performance of a naturally occurring PET-degrading enzyme from the bacterium Piscinibacter sakaiensis. The study suggests that engineered enzymes could eventually help create a circular economy for plastic waste by enabling efficient recycling at the molecular level.
Biểu hiện, tinh sạch và đánh giá sơ bộ hoạt tính phân hủy nhựa PET của enzyme PETase tái tổ hợp
Vietnamese researchers successfully expressed and purified recombinant PETase enzyme — which breaks down PET plastic — finding optimal expression conditions and that adding glycerol and DTT enhanced its plastic-degrading activity. This is directly relevant to microplastic research as PETase-based biodegradation is a promising biological approach to reducing PET plastic waste and microplastic generation.
Increased cytoplasmic expression of PETase enzymes in E. coli.
Researchers optimized the production of PETase — an enzyme that breaks down PET plastic — in E. coli bacteria, achieving higher yields of active enzyme using a bioreactor. Improving enzyme production methods is a key step toward scaling up biological plastic recycling to address PET pollution in the environment.
Degradation of PET plastic with engineered environmental bacteria
Scientists engineered a soil bacterium to break down PET plastic, one of the most common plastics in food packaging and textiles, by giving it the ability to produce and secrete a powerful plastic-degrading enzyme. This is one of the first demonstrations of a living microorganism that can directly consume PET as a food source, which could lead to more sustainable recycling approaches.