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Tier 2
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Original research — experimental, observational, or case-control study. Direct primary evidence.
Gut & Microbiome
Human Health Effects
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In vitro cultivation of primary intestinal cells from Eisenia fetida as basis for ecotoxicological studies
Ecotoxicology2021
15 citations
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Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Score: 35
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0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Julia Jasinski,
Matthias Völkl,
Anja Holzinger,
Anja Holzinger,
Matthias Völkl,
Anja Holzinger,
Anja Holzinger,
S. A. B. Riedl,
S. A. B. Riedl,
S. A. B. Riedl,
S. A. B. Riedl,
Anja Holzinger,
Julia Jasinski,
Julia Jasinski,
Julia Jasinski,
Julia Jasinski,
Julia Jasinski,
Julia Jasinski,
Matthias Völkl,
Valérie Jérôme,
Anja Holzinger,
Matthias Völkl,
Anja Holzinger,
Matthias Völkl,
Thomas Scheibel,
Anja Holzinger,
Heike Feldhaar
Matthias Völkl,
Julia Jasinski,
Matthias Völkl,
Thomas Scheibel,
Anja Holzinger,
Matthias Völkl,
Heike Feldhaar
Anja Holzinger,
Anja Holzinger,
Matthias Völkl,
Valérie Jérôme,
Matthias Völkl,
Matthias Völkl,
Valérie Jérôme,
Matthias Völkl,
Thomas Scheibel,
Heike Feldhaar
Valérie Jérôme,
Thomas Scheibel,
Heike Feldhaar
Heike Feldhaar
Anja Holzinger,
Matthias Völkl,
Julia Jasinski,
Julia Jasinski,
Julia Jasinski,
Julia Jasinski,
Julia Jasinski,
Julia Jasinski,
Julia Jasinski,
Valérie Jérôme,
Valérie Jérôme,
Valérie Jérôme,
Valérie Jérôme,
Valérie Jérôme,
Valérie Jérôme,
Valérie Jérôme,
Ruth Freitag,
Thomas Scheibel,
Valérie Jérôme,
Valérie Jérôme,
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Heike Feldhaar
Ruth Freitag,
Heike Feldhaar
Heike Feldhaar
Heike Feldhaar
Thomas Scheibel,
Thomas Scheibel,
Thomas Scheibel,
Thomas Scheibel,
Valérie Jérôme,
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Thomas Scheibel,
Thomas Scheibel,
Thomas Scheibel,
Heike Feldhaar
Heike Feldhaar
Heike Feldhaar
Heike Feldhaar
Heike Feldhaar
Anja Holzinger,
Thomas Scheibel,
Anja Holzinger,
Heike Feldhaar
Heike Feldhaar
Anja Holzinger,
Thomas Scheibel,
Thomas Scheibel,
Heike Feldhaar
Thomas Scheibel,
Heike Feldhaar
Heike Feldhaar
Heike Feldhaar
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Ruth Freitag,
Heike Feldhaar
Heike Feldhaar
Heike Feldhaar
Heike Feldhaar
Heike Feldhaar
Heike Feldhaar
Thomas Scheibel,
Heike Feldhaar
Thomas Scheibel,
Heike Feldhaar
Summary
Researchers isolated living intestinal cells from earthworms to test how pollutants affect them at the cellular level, finding that silver nanoparticles and heavy metals reduced cell metabolism while microplastic particles (polystyrene and PLA beads) showed only minor effects and were not actively absorbed by the cells.
The earthworm Eisenia fetida is a commonly used model organism for unspecific soil feeders in ecotoxicological studies. Its intestinal cells are the first to encounter possible pollutants co-ingested by the earthworm, which makes them prime candidates for studies of toxic effects of environmental pollutants on the cellular as compared to the organismic level. In this context, the aim of this study was to demonstrate the suitability of preparations of primary intestinal E. fetida cells for in vitro ecotoxicological studies. For this purpose, a suitable isolation and cultivation protocol was established. Cells were isolated directly from the intestine, maintaining >85% viability during subsequent cultivations (up to 144 h). Exposure to established pollutants and soil elutriates comprising silver nanoparticles and metal ions (Cu<sup>2+</sup>, Cd<sup>2+</sup>) induced a significant decrease in the metabolic activity of the cells. In case of microplastic particles (MP particles), namely 0.2, 0.5, 2.0, and 3.0 µm diameter polystyrene (PS) beads as well as 0.5 and 2.0 µm diameter polylactic acid (PLA) beads, no active uptake was observed. Slight positive as well as negative dose and size dependent effects on the metabolism were seen, which to some extent might correlate with effects on the organismic level.