We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Geraniol modulation of oxidative stress caused by polyethylene terephthalate nanoplastics in HEK-293 cell line
Summary
Researchers tested whether geraniol, a natural plant compound, could protect human kidney cells (HEK-293) against oxidative stress caused by polyethylene terephthalate nanoplastics, finding that geraniol significantly reduced nanoplastic-induced cellular damage.
Background: The environmental degradation of plastics into micro- and nanoplastics (MNPs) poses a significant threat to human health. Polyethylene terephthalate nanoplastics (PET-NPs) are of particular concern due to their ability to induce intracellular oxidative stress, a key mechanism of nanoplastic toxicity. Geraniol (3,7-dimethylocta-2,6-dien-1-ol), a natural monoterpenoid, has demonstrated potent antioxidant and cytoprotective properties in various models of chemical-induced toxicity.Objectives: This study aimed to investigate the oxidative stress induced by PET-NPs in human embryonic kidney (HEK-293) cells and to evaluate the potential protective effects of geraniol against this toxicity.Methods: HEK-293 cells were divided into four groups: control, PET-NPs (IC₅₀ = 420.7 µg/mL), geraniol (IC₅₀ = 697.7 µg/mL), and a co-treatment group (PET-NPs + 100 µg/mL geraniol). Following 48 hours of exposure, cytotoxicity was assessed via the MTT and Lactate Dehydrogenase (LDH) assays. Oxidative stress markers, including superoxide dismutase (SOD), catalase (CAT) activities, and total antioxidant capacity (TAC), were measured. The experimental design and reporting adhere to the principles for in vitro toxicology studies.Results: PET-NP exposure significantly increased LDH release (by ~67%) and depleted antioxidant defenses, reducing SOD, CAT, and TAC levels compared to the control (p<0.0001). Co-treatment with geraniol significantly attenuated this damage, reducing LDH release by 23% and restoring TAC by 200% relative to the PET-NP-only group. Geraniol co-treatment also induced a protective shift in the IC₅₀ of PET-NPs from 267.9 µg/mL to 437.6 µg/mL at 72 hours, indicating enhanced cellular resistance.Conclusion: Geraniol effectively mitigates PET-NP-induced cytotoxicity in HEK-293 cells by preserving membrane integrity and augmenting the cellular antioxidant defense system. These findings position geraniol as a promising therapeutic candidate for countering the adverse effects of nanoplastic exposure.
Sign in to start a discussion.
More Papers Like This
Sakuranetin counteracts polyethylene microplastics induced nephrotoxic effects via modulation of Nrf2/Keap1 pathway
Researchers found that polyethylene microplastics caused kidney damage in rats by increasing oxidative stress and disrupting a key protective cellular pathway. However, when the natural plant compound sakuranetin was administered alongside the microplastics, it significantly reduced the kidney damage by restoring antioxidant defenses. The study suggests that certain natural compounds may help counteract some of the harmful effects of microplastic exposure on organ health.
Screening the Toxic effect of Polyethylene Terephthalate Nanoplastics on Kidney of Adult Male Swiss Albino Mice with Promising Betaine Alleviation
Researchers found that polyethylene terephthalate nanoplastics caused significant kidney damage in mice, including elevated markers of renal stress and structural changes to kidney tissue. The study suggests that pre-treatment with betaine, a naturally occurring compound, may help mitigate some of these harmful effects.
Narirutin ameliorates polystyrene microplastics induced nephrotoxicity by modulating oxidative stress, inflammation and Nrf2/Keap1 pathway
Researchers investigated whether narirutin, a natural compound found in citrus fruits, could protect kidneys from damage caused by polystyrene microplastics in rats. The study suggests that microplastic exposure triggered significant kidney stress through oxidation and inflammation, but narirutin helped reduce that damage by activating protective cellular pathways.
Mitigative potential of kaempferide against polyethylene microplastics induced testicular damage by activating Nrf-2/Keap-1 pathway
Researchers tested whether kaempferide, a natural plant compound with antioxidant properties, could protect against testicular damage caused by polyethylene microplastics in rats. They found that the microplastics triggered significant oxidative stress and tissue damage in the testes, but kaempferide treatment substantially reduced these harmful effects by activating a key protective cellular pathway. The study suggests that natural antioxidant compounds may help counteract some of the reproductive harm associated with microplastic exposure.
Attenuative Effects of Ginkgetin Against Polystyrene Microplastics-Induced Renal Toxicity in Rats
Researchers found that ginkgetin, a natural flavonoid, significantly reduced polystyrene microplastic-induced kidney damage in rats by restoring antioxidant enzyme activity and reducing oxidative stress and inflammation markers.