Organ-specific distribution and size-dependent toxicity of polystyrene nanoplastics in Australian bass (Macquaria novemaculeata)

Micro- and nano-plastics (MNPs) are emerging contaminants found in air, water, and food. Ageing and weathering processes convert aquatic plastics into MNPs which, due to their small size, can be assimilated by organisms. The accumulation of MNPs in aquatic life (e.g., fish, oysters, and crabs) will, in turn, pose risks to the health of ecosystems and human. This study focuses on the uptake, biodistribution, and size-dependent toxicity of polystyrene nano-plastics (PS-NPs) in a commercially important food web, the Australian Bass (Macquaria novemaculeata). Fish were fed artemia containing PS-NPs of various sizes (ranging from 50 nm to 1 μm) for durations of 5 and 7 days. The findings revealed that smaller NPs (50 nm) accumulated in the brain and muscle tissues at higher concentrations, whereas larger NPs (1 μm) were primarily found in the gills and intestines. In addition, an inverse correlation was observed between the size of NPs and the rate of trophic transfer, with smaller PS-NPs resulting in a higher transfer rate from artemia to fish. Polystyrene NPs caused both activation of the enzyme superoxide dismutase and damage to the DNA of fish tissues. These effects were size dependent. Metabolomic analysis revealed that indirect exposure to different-sized PS-NPs resulted in altered metabolic profiles within fish intestines, potentially impacting lipid and energy metabolism. These results offer novel perspectives on the size-specific toxic impacts of NPs on fish and the transfer of plastics through the food chain.