Mechanistic insight into interactive effect of microplastics and arsenic on growth of rice (Oryza sativa L.) and soil health indicators

Microplastics (MPs) pollution has recently become a major concern for agroecosystems. The interplay between MPs, and heavy metal(loid)s in the soil can intensify the risks to plant growth and human health. The current study investigated the interactive effects of arsenic (As) and biodegradable and petroleum-based conventional MPs on rice growth, As bioavailability, soil bacterial communities, and soil enzyme activities. As-contaminated soil (5 mg kg) was treated with conventional MPs i.e., polystyrene (PS) and polyethylene (PE) and biodegradable MPs i.e., polylactic acid (PLA) and polybutylene adipate terephthalate (PBAT) at 0.1 % and 1 % rates. In a pot experiment, rice plants were cultivated in soil co-contaminated with As and MPs. PLA-MPs exhibited significant interactions with As, increasing its bioavailability and impairing rice plant growth by enhancing plant oxidative stress. The results illustrated that T2 treatment (PLA-MPs @ 1 % + As 5 mg kg) significantly decreased the root and shoot lengths, root and shoot dry weights as well as the rates of photosynthesis, transpiration, intercellular CO, and stomatal conductance in rice plants. Biodegradable PLA-MPs @ 1 % resulted in increased uptake of As in rice roots, stems, and leaves by 13.4 %, 38.9 %, and 20.6 %, respectively. In contrast, conventional PE-MPs @ 1 % showed contradictory results with As uptake declined by 2.2 %, 5.1 %, and 9.9 % in rice roots, stem and leaves. Soil enzyme kinetics showed that biodegradable MPs increased the activities of soil catalase, dehydrogenase, and phytase enzymes, whereas both conventional PS and PE-MPs decreased their activities. Moreover, As and PLA-MPs combined stress altered soil bacterial communities by increasing the relative abundance of Protobacteria, Acidobacteria, Chloroflexi, and Firmicutes phyla by 49 %, 29 %, 82 %, and 57 %, respectively. This study provides new insights into MPs-As interactions in soil-plant system and ecological risks associated with their coexistence.