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Orbital shaker-driven gut-on-a-chip platform for drug-induced permeability and microenvironment studies
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Researchers developed a simplified gut-on-a-chip platform using an orbital shaker instead of complex pumping systems to study drug effects on intestinal tissue. The device enabled co-culture of gut epithelial and immune cells under realistic flow conditions and was used to assess microplastic-induced changes in gut permeability. The platform offers an accessible tool for studying how environmental contaminants like microplastics affect gut barrier function.
Gut-on-a-chip platforms replicate realistic gut microenvironments but face limited adoption due to their complex designs, expensive fabrication, and specialized instrumentation that increases operational complexity. In this study, we present a microfluidic chip insertable into 12-well plates with a unique radial design and a pumpless flow actuation system using an orbital shaker. We use a surface tension-driven hydrogel patterning technique to compartmentalize the chip, enabling co-culture of gut epithelium and vasculature, resulting in leak-proof monolayer tubes. Furthermore, computational fluid dynamic analysis demonstrates bidirectional peristaltic flow induced by the shaker. The platform's physiological relevance is confirmed through the evaluation of cell polarization, tight junction markers and barrier integrity, using high-magnification microscopy and electrical resistance measurements. We also demonstrate the ability of the platform to support live bacterial colonization, simulating host-microbe interactions. The model is validated for drug development by assessing gut and vascular permeability following drug overdose and inflammatory cytokine activation. Additionally, we explore nanoplastic poisoning using nano polyethylene terephthalate (PET) particles, highlighting the gut's role in limiting particle absorption into the bloodstream. The orbital gut-on-a-chip platform offers an accessible, dynamic cell culture system for drug discovery and biomimetic modeling of gut-related disease interactions.
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Gut‐on‐a‐Chip Reveals Enhanced Peristalsis Reduces Nanoplastic‐Induced Inflammation
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Researchers built a miniature gut-on-a-chip device that mimics intestinal movement to study how nanoplastics affect the digestive system. They found that nanoplastic exposure caused significant inflammation and cell damage in the simulated gut lining. Notably, increasing the intensity of gut-like contractions substantially reduced the inflammatory response, suggesting that healthy intestinal movement may help protect against nanoplastic-related harm.
Impact of Nanoplastic Particles on Intestinal Epithelium Health Using a Vascularized Gut-on-aChip Model
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Researchers developed a vascularized gut-on-a-chip model to evaluate how nanoplastics affect intestinal health, focusing on bioaccumulation, translocation across epithelial barriers, and immune responses at the gut-blood interface — a more physiologically realistic platform than conventional cell culture approaches.
Impact of Nanoplastic Particles on Intestinal Epithelium Health Using a Vascularized Gut-on-aChip Model
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Researchers developed a vascularized gut-on-a-chip model to evaluate how nanoplastics affect intestinal health, focusing on bioaccumulation, translocation across epithelial barriers, and immune responses at the gut-blood interface — a more physiologically realistic platform than conventional cell culture approaches.
Impact of Nanoplastic Particles on Intestinal Epithelium Health Using a Vascularized Gut-on-aChip Model
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Researchers developed a vascularized gut-on-a-chip model to evaluate how nanoplastics affect intestinal health, focusing on bioaccumulation, translocation across epithelial barriers, and immune responses at the gut-blood interface — a more physiologically realistic platform than conventional cell culture approaches.
Gut-on-a-Chip Reveals Reduced Nanoplastic-Induced Inflammation Through Enhanced Peristalsis
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A gut-on-a-chip device with integrated sensors showed that nanoplastics caused cell damage, tight junction loss, and inflammatory cytokine peaks (IL-6, TNF-α) within 24 hours, but that increasing mechanical peristaltic strain from 5% to higher levels significantly reduced these inflammatory responses.
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