We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Effect of Polystyrene Nanoplastics on Ovarian Granulosa Cells
Summary
Researchers exposed human granulosa-like tumor cells to polystyrene nanoplastics at increasing concentrations and measured cell viability, membrane damage, and apoptosis. Nanoplastic exposure reduced cell viability in a dose-dependent manner with an IC50 indicating significant cytotoxicity, suggesting potential harm to female ovarian granulosa cells from nanoplastic exposure.
Existing studies have shown that microplastics can cause male reproductive damage, but the effect of nanoplastics on female reproductive ability is rarely studied. The effect of PS-NPs (polystyrene nanoplastics) on the morphology of human granulosa-like tumor cells (KGN) was observed and photographed by optical microscope. CCK8 and LDH kits were used to detect the viability and damage of KGN cells exposed to 0, 25, 50, 75, 100, 125 and 150 μg/mL of PS-NPs, and the IC50 value of half inhibition of KGN cells by PS-NPs is 130.4 μg/mL. The ROS accumulation in KGN cells after exposure to 200 μg/mL of PS-NPs is significantly increase. The results showed that with the increase of the concentration of 50 nm PS-NPs, the morphology of KGN cells was significantly changed, the cell viability was significantly decreased, and the cell damage rate was significantly increased. These results provide evidence for the toxicity of PS-NPs in female reproduction.
Sign in to start a discussion.
More Papers Like This
The ovarian-related effects of polystyrene nanoplastics on human ovarian granulosa cells and female mice
This study tested the effects of polystyrene nanoplastics on both human ovarian cells in the lab and on female mice. The nanoplastics accumulated in ovarian tissue, caused cell death, disrupted hormone levels, and reduced egg quality and fertility in mice. These findings suggest that nanoplastic exposure could threaten female reproductive health by damaging the ovaries.
Toxicity of polystyrene nanoparticles for mouse ovary and cultured human granulosa cells
Researchers investigated the effects of polystyrene nanoparticles on female reproductive health using both mouse ovaries and human granulosa cell cultures. They found that nanoparticle exposure damaged ovarian tissue, reduced egg quality, and triggered cell death through oxidative stress and inflammation pathways. The study suggests that nanoplastic exposure may pose risks to female fertility, though more research is needed to confirm effects at real-world exposure levels.
Nanoplastics impair in vitro swine granulosa cell functions
Polystyrene nanoplastics at the highest tested concentration (75 µg/mL) stimulated cell proliferation and steroid hormone secretion in swine granulosa cells while also increasing oxidative stress, suggesting potential endocrine disruption in female reproductive cells.
Polystyrene nanoplastics induce apoptosis, autophagy, and steroidogenesis disruption in granulosa cells to reduce oocyte quality and fertility by inhibiting the PI3K/AKT pathway in female mice
Researchers found that polystyrene nanoplastics (tiny plastic particles under 1 micrometer) impair egg cell quality in female mice by damaging the ovarian support cells that help eggs mature, triggering cell death and disrupting hormone production. These findings raise important questions about the potential reproductive risks of nanoplastic exposure in women.
Exposure to polystyrene nanoplastics induces lysosomal enlargement and lipid droplet accumulation in KGN human ovarian granulosa cells
Researchers exposed human ovarian cells to polystyrene nanoplastics and found that the particles entered the cells and caused abnormal enlargement of lysosomes (cellular recycling structures) and accumulation of fat droplets. These changes occurred even at concentrations that did not kill the cells outright, suggesting subtle but potentially significant damage. The findings point to a possible mechanism by which nanoplastics could impair female reproductive health.