We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Nanoplastics impair in vitro swine granulosa cell functions
Summary
Polystyrene nanoplastics at the highest tested concentration (75 µg/mL) stimulated cell proliferation and steroid hormone secretion in swine granulosa cells while also increasing oxidative stress, suggesting potential endocrine disruption in female reproductive cells.
Soil, water, and air pollution by plastic represents an issue of great concern since the particles produced by degradation of plastic materials can be ingested by animals and humans, with still uncertain health consequences. As a contribution on this crucial subject, the present work reports an investigation on the in vitro effects of different concentrations of polystyrene nanoplastics (5, 25, and 75 µg/mL) on swine granulosa cells, a model of endocrine reproductive cells. In particular, cell growth (BrDU incorporation and ATP production), steroidogenesis (17-β estradiol and progesterone secretion) and redox status (superoxide and nitric oxide production, enzymatic and non-enzymatic scavenging activity) were studied. Nanoplastics, at the highest concentration, stimulated cell proliferation (P < 0.05), while cell viability resulted unaffected. Steroidogenesis was disrupted (P < 0.05). Both enzymatic and non-enzymatic scavenging activity were increased after exposure at the highest nanoplastic dose (P < 0.05, P < 0.001). Nitric oxide secretion was increased by 25 and 75 µg/mL (P < 0.05) while superoxide generation was stimulated (P < 0.001) only by the highest concentration tested. Taken together, main features of cultured swine granulosa cells resulted affected by exposure to nanoplastics. These results raise concerns since environment nanoplastic contamination can represents a serious threat to animal and human health.
Sign in to start a discussion.
More Papers Like This
Effect of Polystyrene Nanoplastics on Ovarian Granulosa Cells
Researchers exposed human granulosa-like tumor cells to polystyrene nanoplastics at increasing concentrations and measured cell viability, membrane damage, and apoptosis. Nanoplastic exposure reduced cell viability in a dose-dependent manner with an IC50 indicating significant cytotoxicity, suggesting potential harm to female ovarian granulosa cells from nanoplastic exposure.
The ovarian-related effects of polystyrene nanoplastics on human ovarian granulosa cells and female mice
This study tested the effects of polystyrene nanoplastics on both human ovarian cells in the lab and on female mice. The nanoplastics accumulated in ovarian tissue, caused cell death, disrupted hormone levels, and reduced egg quality and fertility in mice. These findings suggest that nanoplastic exposure could threaten female reproductive health by damaging the ovaries.
Polystyrene microplastics cause granulosa cells apoptosis and fibrosis in ovary through oxidative stress in rats
Researchers exposed female rats to polystyrene microplastics at different concentrations for 90 days and examined the effects on their ovaries. The study found that microplastic exposure caused cell death and tissue scarring in the ovaries through oxidative stress, suggesting that microplastics may have implications for female reproductive health.
Toxicity of polystyrene nanoparticles for mouse ovary and cultured human granulosa cells
Researchers investigated the effects of polystyrene nanoparticles on female reproductive health using both mouse ovaries and human granulosa cell cultures. They found that nanoparticle exposure damaged ovarian tissue, reduced egg quality, and triggered cell death through oxidative stress and inflammation pathways. The study suggests that nanoplastic exposure may pose risks to female fertility, though more research is needed to confirm effects at real-world exposure levels.
Effect of polystyrene nanoplastics on in vitro maturation of pig cumulus-encosed oocytes
Researchers exposed pig egg cells to polystyrene nanoplastics during laboratory maturation and found that while the eggs still completed their basic development stages, the nanoplastics significantly increased harmful reactive oxygen species levels. This oxidative stress reduced the quality of resulting embryos, as indicated by fewer cells per embryo, suggesting nanoplastic exposure could be a concern for reproductive health.