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Supplemental data for: Comparison of microplastics in wastewater influent and effluent from wastewater treatment plants in Ontario, Canada

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This is supplemental data for a study of six wastewater treatment plants in southern Ontario, Canada, which tested water entering and leaving the plants for microplastics. Treatment reduced microplastic levels by 91.9% to 98.5%, though fibers became relatively more common in treated water, and smaller particles were harder to remove than larger ones.

Study Type Environmental

Wastewater treatment plants (WWTPs) are important pathways for microplastics (MPs) to enter aquatic environments. However, MP occurrence and removal remain poorly characterized in Ontario, Canada, particularly with respect to changes in particle abundance and characteristics between influent and effluent. This study compared MP abundance and particle characteristics in influent and effluent from six WWTPs across southern Ontario, representing a range of facility sizes and treatment configurations. Influent and effluent samples were collected in 2022 and processed using vacuum filtration, with influent samples undergoing additional digestion to remove organic matter. Suspected MPs were quantified and characterized by morphology, colour, and size using optical microscopy, and Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy was used to verify the polymer composition of a subset of particles. MPs were detected in all influent and effluent samples, with mean concentrations of 1,790.2 ± 729.5 MP/L and 74.6 ± 30.4 MP/L respectively. Significant reductions in MP abundance between influent and effluent were observed at all six WWTPs, ranging from 91.9% to 98.5%. Fibres (50.8%) and fragments (37.9%) were the most abundant particle morphologies, with the relative abundance of fibres increasing between influent and effluent at all six facilities. Additionally, MP abundance generally increased with decreasing particle size in both influent and effluent samples across all six WWTPs. Of the 600 particles analyzed with ATR-FTIR, 65.3% were confirmed as synthetic polymers, with polyester (39.0%), polypropylene (16.3%), polystyrene (15.3%), and polyethylene (14.5%) being the most abundant across all WWTPs. Overall, these finding show that the tested WWTPs greatly reduced MP concentrations between influent and effluent. However, there is evidence that differences in MP characteristics such as size and morphology may affect their removal efficiency.

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