Studies on the impact of aged microplastics on agricultural soil enzyme activity, lettuce growth, and oxidative stress

Microplastics (MPs) represent an increasingly significant source of pollution, with their ubiquitous presence not only contaminating soil but also influencing plant growth. To elucidate the effects of MPs on soil-plant systems, this study examined the impact of exposure to aged polystyrene (PS), polyethylene, and polylactic acid (PLA) MPs at varying concentrations (0.1%, 1%, 5%, and 10%) on soil physicochemical properties, enzyme activities, lettuce growth, and oxidative stress conditions in a pot experiment. The results indicated that high concentrations (5% and 10%) of PLA increased soil urease activity by 18.27% and 23.57%, respectively, whereas PS reduced it by 12.02% and 27.15%, respectively, compared to the control. High concentrations (5% and 10%) of PLA reduced the fresh weight of lettuce leaves and roots by 58.38-61.08% and 49.20-51.68%, respectively. The addition of all three MPs increased the soluble sugar content in lettuce leaves by 34.10-65.30%. The presence of all three types of MPs significantly enhanced catalase (CAT) and superoxide dismutase (SOD) activities in lettuce leaves at concentrations of 0.1%, 1%, and 5%, with the greatest increase in SOD activity (26.06-31.34%) observed at the 5% concentration. Root CAT activity was elevated at low concentrations (0.1% and 1%), whereas 10% PLA significantly suppressed both CAT and SOD activities. Integrated biomarker response analysis showed that MPs induced oxidative stress in lettuce. The results of this study provide a theoretical basis for evaluating the potential ecological risks posed by MPs to the soil-plant system.