We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Establishment and characterization of an ovarian cell line from red seabream (Pagrus major) and its application in fish toxicology
AI summary Read the abstract
Scientists created a new lab tool using fish ovary cells to study how microplastics affect living tissue, and found that smaller plastic particles were absorbed into cells more easily, while higher concentrations caused more cell death. Since fish are a major food source and part of the same waters humans rely on, this tool could help researchers better understand how the microplastics polluting our oceans might impact living organisms—including, potentially, us.
Fish cell lines serve as ideal in vitro models for investigating the toxicity of environmental pollutants in fish. In this study, a novel cell line (PmOVC) derived from the ovary of red seabream (Pagrus major) was successfully established. Using polystyrene microplastics (PS-MPs)-a typical environmental pollutant-as the test substance, the toxicity sensitivity of the PmOVC cell line was systematically assessed from the dimensions of particle size intake and cell activity. The results showed that the PmOVC cells exhibited robust growth in M199 medium supplemented with 15% FBS at 23°C and high exogenous gene transfection efficiency and showed high sensitivity to the toxic effects of microplastics. Specifically, the smaller the particle size of microplastics, the higher the internalization efficiency of cells, among which 100 nm PS-MPs could be completely endocytosed into the cytoplasm; the concentration of PS-MPs was significantly positively correlated with the apoptosis rate of cells (R ≥ 0.8). In summary, the established PmOVC cell line provides a critical in vitro tool for in-depth investigation of the toxicological responses and underlying mechanisms of fish to environmental pollutants.
More Papers Like This
First evidence of in vitro cytotoxic effects of marine microlitter on Merluccius merluccius and Mullus barbatus , two Mediterranean commercial fish species
AI summary Read the abstract
Researchers collected actual marine litter (not lab-made microplastics) from the Northern Adriatic Sea and tested its toxicity on cells from two commercially caught fish species. Real marine microlitter caused significant cell damage and inflammation, including effects not seen with pristine lab-grade microplastics. This suggests that studies using only clean, standardized plastic particles may underestimate the true toxicity of environmental microplastics.
Atlantic salmon gill epithelial cell line ASG-10, an in vitro model for studying effects of microplastics in gills
AI summary Read the abstract
Researchers used an Atlantic salmon gill cell line to study how fish gill cells interact with polystyrene microplastics of different sizes. They found that cells could take up particles at 0.2 and 1.0 micrometers but not at 10 micrometers, and the uptake triggered increased activity in the cells' waste-processing systems. The study establishes this cell line as a useful laboratory model for investigating how microplastics affect fish gills without requiring live animal testing.
Toxicity assessment of pollutants sorbed on microplastics using various bioassays on two fish cell lines
AI summary Read the abstract
Researchers collected microplastic samples from ocean expeditions and tested their toxicity using two fish cell lines, finding that cell lines differed in sensitivity and that microplastics with sorbed pollutants were toxic to cells. The results suggest that real-world microplastics carrying accumulated chemical pollutants pose a chemical toxicity risk to marine organisms beyond just the physical effects of ingesting plastic.
Size-dependent internalization of micro- and nanoplastics induces pro-inflammatory and oxidative stress responses in marine and freshwater fish cell lines
AI summary Read the abstract
Scientists found that tiny plastic particles (microplastics) can slip inside fish cells and trigger inflammation and stress responses, even though the cells didn't die. Smaller particles got in more slowly but still caused problems, suggesting that as plastics break down into smaller pieces in the environment, they may still be harmful even if they don't kill cells outright. Since we eat fish and are exposed to similar tiny plastics ourselves, this research hints that microplastics could be quietly stressing our cells too, even without obvious damage.
Research digests by email
When a large batch of papers lands in the Atlas, we read through it and send a short write-up of what stood out.