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Atlantic salmon gill epithelial cell line ASG-10, an in vitro model for studying effects of microplastics in gills
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Researchers used an Atlantic salmon gill cell line to study how fish gill cells interact with polystyrene microplastics of different sizes. They found that cells could take up particles at 0.2 and 1.0 micrometers but not at 10 micrometers, and the uptake triggered increased activity in the cells' waste-processing systems. The study establishes this cell line as a useful laboratory model for investigating how microplastics affect fish gills without requiring live animal testing.
Microplastics are ubiquitous environmental pollutants frequently detected in aquatic environments. Here we used the Atlantic salmon epithelial gill cell line (ASG-10) to investigate the uptake and effects of polystyrene (PS) microplastic. The ASG-10 cell line has phagocytotic/endocytic capacities and can take up clear PS particles at 0.2 and 1.0 µm, while PS at 10 µm was not taken up. As a response to the uptake, the ASG-10 cells increased their lysosomal activity. Furthermore, no effects on the mitochondria were found, neither on the mitochondrial membrane potential nor the mitochondria morphology (branch length and diameter). Interestingly, even a very high concentration of PS (200 µg/ml) with all tested particle sizes had no effects on cell viability or cell cycle. The environmental toxin Benzo(a)pyrene (B(a)P), a known inducer of CYP1A, is highly hydrophobic and thus sticks to the PS particles. However, co-exposure of B(a)P and PS the particles did not increase the induction of CYP1A activity compared to B(a)P alone. Our study contributes to the understanding of the cellular effects of PS particles using a highly relevant Atlantic salmon gill epithelium in vitro model.
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Additional file 1 of Uptake of different-sized microplastic particles in skin and corneal epithelial cells from Atlantic salmon
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I don't have access to the actual content of this paper—only its title and a note that the abstract is a supplementary file with no substantive text provided. Based on the title alone, this appears to be a lab study looking at whether skin and eye (corneal) cells from Atlantic salmon absorb tiny plastic particles of different sizes, likely to understand how microplastics might affect fish health. **A note on relevance to humans:** Without the full findings, I can't say whether or how this connects to human health—fish cell studies don't automatically translate to people. If you can share the actual abstract or key results, I can give you an accurate, specific summary of what the
Additional file 1 of Uptake of different-sized microplastic particles in skin and corneal epithelial cells from Atlantic salmon
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I don't have access to the actual content of this paper — only the title and a placeholder abstract ("Additional file 1"), which appears to be a supplementary data file rather than the full study. Could you provide the main paper's abstract or key findings? That way I can give you an accurate, plain-language summary rather than guessing at what the researchers actually discovered. That said, based on the title alone, here's what I can infer with appropriate caution: this research looked at how tiny plastic particles of different sizes get absorbed into skin and eye cells from Atlantic salmon. If microplastics can enter fish tissues through the skin and eyes, it
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