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20 resultsShowing papers similar to Recent advances in enzyme engineering for improved deconstruction of poly(ethylene terephthalate) (PET) plastics
ClearAn Overview into Polyethylene Terephthalate (PET) Hydrolases and Efforts in Tailoring Enzymes for Improved Plastic Degradation
This review examines the discovery and engineering of PET-degrading enzymes including PETase and cutinase variants, discussing protein engineering strategies to improve catalytic efficiency and thermostability for practical biodegradation of polyethylene terephthalate plastic waste.
Engineered polyethylene terephthalate hydrolases: perspectives and limits
This review examines progress in engineering enzymes that can break down PET plastic, the material used in most beverage bottles and synthetic textiles. Researchers found that while significant advances have been made through protein engineering and machine learning, no enzyme yet exists that can efficiently degrade the crystalline form of PET found in real-world waste. The study outlines the key challenges remaining before enzymatic plastic recycling can work at industrial scale, including handling microplastic contamination.
Determinants for an Efficient Enzymatic Catalysis in Poly(Ethylene Terephthalate) Degradation
This review covers the current state of enzymatic PET degradation, examining which enzymes act on PET, how protein engineering has improved their activity, and what challenges remain before enzymatic recycling can be deployed at industrial scale.
Enzymatic PET Degradation
This review examines enzymatic degradation of PET (polyethylene terephthalate), the plastic used in bottles and polyester clothing, as a promising pathway for breaking down this persistent polymer. Advances in engineering more efficient PET-degrading enzymes could enable industrial-scale biological recycling and reduce the environmental accumulation of PET microplastics.
Advancing PET-Degrading Enzymes through Directed Evolution to Combat Plastic Pollution
This review examines advances in directed evolution of PET-degrading enzymes including PETases and cutinases, describing how techniques such as error-prone PCR, DNA shuffling, and saturation mutagenesis have produced enzyme variants with improved catalytic efficiency and thermostability for enzymatic plastic recycling applications.
Enzymatic Remediation of Polyethylene Terephthalate (PET)–Based Polymers for Effective Management of Plastic Wastes: An Overview
Enzymatic approaches for remediating PET-based plastic waste were reviewed, covering PETase and related enzymes that can break PET into reusable monomers. Enzyme engineering strategies to improve thermostability and catalytic efficiency are discussed as a pathway to scalable biological PET recycling.
Computational redesign of a PETase for plastic biodegradation by the GRAPE strategy
Researchers engineered a more stable version of the enzyme PETase, which breaks down PET plastic, using a computational protein design strategy. The improved enzyme could enable more efficient industrial biodegradation of PET plastic waste, including microplastics.
Genes for a Circular and Sustainable Bio-PET Economy
This review examines the genetics of enzymes that can biodegrade PET plastic, exploring how genetic engineering could accelerate the development of organisms capable of breaking down plastic waste. Enzymatic degradation of PET could help address plastic pollution including plastic bottles that break down into microplastics.
An efficient strategy to tailor PET hydrolase: Simple preparation with high yield and enhanced hydrolysis to micro-nano plastics
This study developed a simplified, high-yield preparation method for PET-degrading hydrolase enzymes to improve their ability to break down PET nano- and microplastics. The engineered enzyme showed enhanced hydrolysis activity against PET microplastics, offering a more practical route to enzymatic plastic waste treatment.
Development of Enzyme-Based Approaches for Recycling PET on an Industrial Scale
This paper reviews the development of enzyme-based methods for breaking down PET plastic (used in bottles and packaging) at an industrial scale. While enzymatic recycling is a promising solution to plastic waste, current methods are still too slow and costly for widespread use. Improving these technologies could help reduce the enormous amount of PET entering the environment and breaking down into microplastics.
Recent advances in screening and identification of PET-degrading enzymes
Researchers reviewed recent advances in discovering and engineering enzymes capable of breaking down PET plastic, one of the most widely produced and persistent plastic types. They examined screening methods including metagenomic mining and machine learning approaches that have accelerated the identification of promising PET-degrading enzymes. The study suggests that enzymatic recycling could become a viable, environmentally friendly alternative to traditional PET disposal methods.
Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET
This review discusses the role of enzymatic biocatalysis in PET plastic degradation, examining how the discovery of PETase and subsequent enzyme engineering have advanced biodegradation as an alternative to chemical and mechanical recycling for one of the most produced plastics globally.
Recent trends in microbial and enzymatic plastic degradation: a solution for plastic pollution predicaments
This review covers recent advances in using microorganisms and their enzymes to break down plastics including polyethylene, PVC, polystyrene, and PET, with techniques like protein engineering being used to boost enzyme efficiency. Microbial degradation offers a sustainable approach to reducing the persistent plastic pollution that generates the microplastics found throughout the environment and human body.
Discovery and mechanism-guided engineering of BHET hydrolases for improved PET recycling and upcycling
Researchers identified and engineered two enzymes — called BHETases — that efficiently break down PET plastic (the kind used in bottles and packaging) into its chemical building blocks, achieving up to seven times better output than leading existing enzymes. By coupling these improved enzymes in a two-step system, the team demonstrated a path toward true closed-loop PET recycling.
Applications of Synthetic Biology in Microbial and Enzymatic Systems for Microplastic Degradation: A Review
This review examines how synthetic biology is being used to enhance the biological breakdown of microplastics, covering advances in enzyme engineering, whole-cell engineering, and metabolic pathway design. Researchers have achieved significant improvements in plastic-degrading enzymes like PETase through directed evolution and machine learning, enabling depolymerization of consumer plastics under increasingly mild industrial conditions.
Eco-Microbiology: Discovering Biochemical Enhancers of PET Biodegradation by Piscinibacter sakaiensis
This paper reviews biochemical strategies for enhancing PET biodegradation by microorganisms, focusing on the discovery and engineering of plastic-degrading enzymes. The review highlights recent advances and remaining challenges in scaling up enzymatic plastic degradation for industrial applications.
Explorations of Polyethylene Terephthalate (PET) Hydrolase for addressing PET Plastic Pollution
This review explores the biology of PETase enzymes and their potential for addressing PET plastic pollution, covering the discovery of Ideonella sakaiensis and subsequent enzyme engineering efforts. Developing efficient PET-degrading enzymes is a promising biotechnological strategy for reducing plastic pollution at scale.
Current Knowledge on Polyethylene Terephthalate Degradation by Genetically Modified Microorganisms
This review covers genetically modified microorganisms engineered to degrade polyethylene terephthalate, examining how bioengineering of enzymes such as PETase and enhanced expression systems can overcome the low biodegradation rates of wild-type microorganisms toward this ubiquitous plastic.
Enhancing PET Degrading Enzymes: A Combinatory Approach
Scientists worked on improving enzymes that can break down PET plastic, one of the most common plastics in consumer products. Using a combinatory approach, researchers enhanced the performance of a naturally occurring PET-degrading enzyme from the bacterium Piscinibacter sakaiensis. The study suggests that engineered enzymes could eventually help create a circular economy for plastic waste by enabling efficient recycling at the molecular level.
Application of PETase in Plastic Biodegradation and Its Synthesis
This review examines how PETase enzymes can be used to biodegrade plastic waste, particularly polyethylene terephthalate, which is one of the most widely used plastics globally. Researchers discuss recent advances in modifying PETase enzymes for improved efficiency and establishing sustainable synthesis platforms. The study suggests that enzymatic biodegradation offers a promising biological solution to the growing plastic pollution crisis.