Papers

61,005 results
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Article Tier 2

An Overview into Polyethylene Terephthalate (PET) Hydrolases and Efforts in Tailoring Enzymes for Improved Plastic Degradation

This review examines the discovery and engineering of PET-degrading enzymes including PETase and cutinase variants, discussing protein engineering strategies to improve catalytic efficiency and thermostability for practical biodegradation of polyethylene terephthalate plastic waste.

2022 International Journal of Molecular Sciences 120 citations
Article Tier 2

Enzymatic Remediation of Polyethylene Terephthalate (PET)–Based Polymers for Effective Management of Plastic Wastes: An Overview

Enzymatic approaches for remediating PET-based plastic waste were reviewed, covering PETase and related enzymes that can break PET into reusable monomers. Enzyme engineering strategies to improve thermostability and catalytic efficiency are discussed as a pathway to scalable biological PET recycling.

2020 Frontiers in Bioengineering and Biotechnology 193 citations
Article Tier 2

Microbial Polyethylene Terephthalate Hydrolases: Current and Future Perspectives

This review surveys microbial enzymes capable of breaking down PET plastic, focusing on the structure and function of key hydrolases like PETase and cutinases. Researchers found that while several enzymes show promising PET-degrading activity, most work slowly and under limited temperature conditions, with engineered variants showing improved performance. The study highlights both the potential and the current limitations of using biological approaches for plastic waste management.

2020 Frontiers in Microbiology 168 citations
Article Tier 2

Current knowledge on enzymatic PET degradation and its possible application to waste stream management and other fields

This review distinguished between enzymatic PET surface modification (useful for fiber treatment) and enzymatic PET degradation (needed for waste management), cataloguing the hydrolases capable of each function and the conditions required. The authors evaluate the prospects for deploying PET-degrading enzymes in industrial plastic waste streams.

2019 Applied Microbiology and Biotechnology 576 citations
Article Tier 2

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET

This review discusses the role of enzymatic biocatalysis in PET plastic degradation, examining how the discovery of PETase and subsequent enzyme engineering have advanced biodegradation as an alternative to chemical and mechanical recycling for one of the most produced plastics globally.

2021 International Journal of Molecular Sciences 97 citations
Article Tier 2

Determinants for an Efficient Enzymatic Catalysis in Poly(Ethylene Terephthalate) Degradation

This review covers the current state of enzymatic PET degradation, examining which enzymes act on PET, how protein engineering has improved their activity, and what challenges remain before enzymatic recycling can be deployed at industrial scale.

2023 Catalysts 19 citations
Article Tier 2

Microbial and Enzymatic Degradation of Plastic Waste in Water

This review surveys microbial and enzymatic pathways for degrading plastic waste in water, cataloging enzymes such as PETases and cutinases along with the microorganisms that produce them. The authors assess current limitations of biological degradation rates and discuss how enzyme engineering and synthetic microbial consortia could accelerate plastic breakdown.

2024
Article Tier 2

Microbial enzymes for the recycling of recalcitrant petroleum‐based plastics: how far are we?

This review examines the progress in identifying microbial enzymes capable of breaking down petroleum-based plastics like polyethylene, polystyrene, polyurethane, and PET. Researchers highlight recent advances in using polyester-degrading enzymes to recover raw materials from PET waste through biocatalytic recycling. The study discusses the potential and remaining challenges of using biological approaches to address the growing global problem of plastic waste accumulation.

2017 Microbial Biotechnology 788 citations
Article Tier 2

Hydrolytic Degradation of Polyethylene Terephthalate by Cutinase Enzyme Derived from Fungal Biomass–Molecular Characterization

Researchers isolated cutinase and lipase enzymes from Aspergillus tamarii and Penicillium crustosum fungi and demonstrated their ability to catalyze hydrolytic degradation of PET plastic, offering a potential biological route for plastic waste breakdown.

2021 Biointerface Research in Applied Chemistry 37 citations
Article Tier 2

Biodegradation of Plastic and the Role of Microbial Enzymes in Plastic Waste Management

This review examines how microbial enzymes, particularly PET hydrolases and oxidative enzymes, can depolymerize and break down common plastic polymers through biological degradation. The study suggests that enzymatic approaches to plastic waste management offer a promising complement to mechanical and chemical recycling, though optimizing enzyme activity and scaling up the process remain key challenges.

2026 International Journal of Education Management and Technology
Article Tier 2

An efficient strategy to tailor PET hydrolase: Simple preparation with high yield and enhanced hydrolysis to micro-nano plastics

This study developed a simplified, high-yield preparation method for PET-degrading hydrolase enzymes to improve their ability to break down PET nano- and microplastics. The engineered enzyme showed enhanced hydrolysis activity against PET microplastics, offering a more practical route to enzymatic plastic waste treatment.

2024 International Journal of Biological Macromolecules 5 citations
Article Tier 2

Enzymatic Degradation of PET plastic

This study tested commercial-grade enzymes for degrading PET plastic and found that enzymatic degradation was effective at laboratory scale but faced challenges for real-world application. Scaling up enzymatic PET recycling could reduce the persistence of plastic waste that eventually fragments into microplastics in the environment.

2023 ARPHA Conference Abstracts
Article Tier 2

Advancing PET-Degrading Enzymes through Directed Evolution to Combat Plastic Pollution

This review examines advances in directed evolution of PET-degrading enzymes including PETases and cutinases, describing how techniques such as error-prone PCR, DNA shuffling, and saturation mutagenesis have produced enzyme variants with improved catalytic efficiency and thermostability for enzymatic plastic recycling applications.

2025 American journal of student research.
Article Tier 2

Recent advances in screening and identification of PET-degrading enzymes

Researchers reviewed recent advances in discovering and engineering enzymes capable of breaking down PET plastic, one of the most widely produced and persistent plastic types. They examined screening methods including metagenomic mining and machine learning approaches that have accelerated the identification of promising PET-degrading enzymes. The study suggests that enzymatic recycling could become a viable, environmentally friendly alternative to traditional PET disposal methods.

2024 Environmental Reviews 7 citations
Article Tier 2

Genes for a Circular and Sustainable Bio-PET Economy

This review examines the genetics of enzymes that can biodegrade PET plastic, exploring how genetic engineering could accelerate the development of organisms capable of breaking down plastic waste. Enzymatic degradation of PET could help address plastic pollution including plastic bottles that break down into microplastics.

2019 Preprints.org 17 citations
Article Tier 2

Development of Enzyme-Based Approaches for Recycling PET on an Industrial Scale

This paper reviews the development of enzyme-based methods for breaking down PET plastic (used in bottles and packaging) at an industrial scale. While enzymatic recycling is a promising solution to plastic waste, current methods are still too slow and costly for widespread use. Improving these technologies could help reduce the enormous amount of PET entering the environment and breaking down into microplastics.

2024 Biochemistry 22 citations
Article Tier 2

Enzymatic PET Degradation

This review examines enzymatic degradation of PET (polyethylene terephthalate), the plastic used in bottles and polyester clothing, as a promising pathway for breaking down this persistent polymer. Advances in engineering more efficient PET-degrading enzymes could enable industrial-scale biological recycling and reduce the environmental accumulation of PET microplastics.

2019 CHIMIA International Journal for Chemistry 50 citations
Article Tier 2

Potential Use of Microbial Enzymes for the Conversion of Plastic Waste Into Value-Added Products: A Viable Solution

This review examines microbial enzymes capable of hydrolyzing PLA-PET mixed plastic waste, evaluating enzymatic degradation pathways and the potential for enzyme-based plastic valorization as an economically viable alternative to chemical recycling.

2021 Frontiers in Microbiology 66 citations
Article Tier 2

Biodegradation of PET: Current Status and Application Aspects

This review covered the current status of PET biodegradation, focusing on the discovery and mechanism of PET hydrolytic enzymes from Ideonella sakaiensis and the pathway from enzyme identification to potential bioremediation application. The authors assess progress toward commercializing enzymatic PET degradation and identify remaining technical barriers for large-scale plastic waste management.

2019 ACS Catalysis 614 citations
Article Tier 2

Marine PET Hydrolase (PET2): Assessment of Terephthalate- and Indole-Based Polyester Depolymerization

This study characterized a marine-derived enzyme (PET2) capable of breaking down PET plastic under mild conditions, assessing its efficiency for enzymatic recycling. Enzyme-based PET recycling could prevent plastic waste from fragmenting into the microplastics that accumulate in oceans and organisms.

2023 Catalysts 2 citations
Article Tier 2

Eco-Microbiology: Discovering Biochemical Enhancers of PET Biodegradation by Piscinibacter sakaiensis

This paper reviews biochemical strategies for enhancing PET biodegradation by microorganisms, focusing on the discovery and engineering of plastic-degrading enzymes. The review highlights recent advances and remaining challenges in scaling up enzymatic plastic degradation for industrial applications.

2024
Article Tier 2

Engineered polyethylene terephthalate hydrolases: perspectives and limits

This review examines progress in engineering enzymes that can break down PET plastic, the material used in most beverage bottles and synthetic textiles. Researchers found that while significant advances have been made through protein engineering and machine learning, no enzyme yet exists that can efficiently degrade the crystalline form of PET found in real-world waste. The study outlines the key challenges remaining before enzymatic plastic recycling can work at industrial scale, including handling microplastic contamination.

2024 Applied Microbiology and Biotechnology 27 citations
Article Tier 2

Application of PETase in Plastic Biodegradation and Its Synthesis

This review examines how PETase enzymes can be used to biodegrade plastic waste, particularly polyethylene terephthalate, which is one of the most widely used plastics globally. Researchers discuss recent advances in modifying PETase enzymes for improved efficiency and establishing sustainable synthesis platforms. The study suggests that enzymatic biodegradation offers a promising biological solution to the growing plastic pollution crisis.

2024 E3S Web of Conferences 2 citations
Article Tier 2

Marine PET Hydrolase (PET2): Assessment of Terephthalate- and Indole-Based Polyesters Depolymerization

Researchers characterized a marine enzyme (PET2) capable of breaking down PET plastic and related polyester materials under relatively mild conditions. Discovering and engineering enzymes that can degrade PET could help address the massive accumulation of PET microplastics in ocean environments.

2023 Preprints.org 1 citations