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61,005 resultsShowing papers similar to In Situ Identification and Spatial Mapping of Microplastic Standards in Paramecia by Secondary-Ion Mass Spectrometry Imaging
ClearIn situ imaging of microplastics in living organisms based on mass spectrometry technology
Researchers reviewed mass spectrometry-based imaging techniques for detecting microplastics inside living organisms, comparing different ion source methods for their ability to visualize plastic particles in biological tissue. They found that these techniques can provide both spatial distribution maps and chemical composition analysis of microplastics at high resolution. The study suggests that mass spectrometry imaging could become a powerful tool for understanding how microplastics accumulate and distribute within living systems.
Mass spectrometry imaging enables detection of MPs and their effects in Daphnia magna following acute exposure
Researchers used an advanced imaging technique called mass spectrometry imaging to track where microplastics accumulate inside water fleas after short-term exposure. They found that the tiny organisms ingested microplastics that concentrated in their gut, and the exposure altered their lipid metabolism. The technique offers a new way to visualize exactly where microplastics end up in small aquatic organisms and what biochemical changes they cause.
Localisation and identification of polystyrene particles in tissue sections using Raman spectroscopic imaging
Researchers developed a Raman spectroscopic imaging method to localize and identify polystyrene microplastic particles directly within tissue sections, enabling in-situ detection without fluorescent labeling and making environmental sample analysis feasible.
Label-free stimulated Raman scattering imaging of intracellular microplastics in mammalian cells
Researchers used label-free stimulated Raman scattering imaging to visualize microplastic uptake and distribution inside mammalian cells without fluorescent labels, finding that intracellular microplastics were associated with elevated reactive oxygen species, reduced cell viability, and altered lipid metabolism.
Development of Water Cluster-Secondary Ion Mass Spectrometry and Particle Induced X-ray Emission to Investigate Spatially Resolved Biological Responses to Nanopolystyrene in Zebrafish Larvae
Researchers developed advanced imaging techniques using water cluster-secondary ion mass spectrometry and particle-induced X-ray emission to study how nanoplastics interact with zebrafish larvae at the tissue level. They mapped the spatial distribution of elements and molecular changes in organs exposed to nanopolystyrene at environmentally realistic concentrations. The study provides new analytical methods for understanding how nanoplastics are taken up and distributed in living organisms, filling a critical gap in nanoplastic toxicology research.
Imaging and quantifying the biological uptake and distribution of nanoplastics using a dual-functional model material
Researchers developed a dual-functional nanoplastic model material that allows both imaging and precise quantification of nanoplastic uptake in biological systems. Using surface-enhanced Raman spectroscopy and inductively coupled plasma mass spectrometry, they could track where nanoplastics accumulated in organisms at high resolution. The tool addresses a major gap in nanoplastic research by enabling more accurate measurement of how these tiny particles interact with living tissues.
Synchrotron-based Spectromicroscopy for Microplastic Detection and Characterization
Researchers reviewed how synchrotron-based imaging techniques — which use powerful X-ray beams to see extremely fine details — can detect and chemically identify micro- and nanoplastics that conventional methods miss, including plastics absorbed into biological tissues. These high-resolution tools are still in early stages but show strong potential for mapping microplastic contamination at the nanoscale.
Label-Free Identification and Imaging of Microplastic and Nanoplastic Biouptake Using Optical Photothermal Infrared Microspectroscopy
Researchers developed a new imaging technique that can locate and identify microplastic and nanoplastic particles inside whole organisms without needing fluorescent labels. Using a method called optical photothermal infrared microscopy, they tracked polystyrene particles as small as 1 micrometer in roundworms. This tool could help scientists better understand how plastic particles are taken up by living things and where they accumulate in the body.
Spectro‐Microscopic Techniques for Studying Nanoplastics in the Environment and in Organisms
This review examines spectro-microscopic techniques for detecting and characterizing nanoplastics (under 1 um) in environmental and biological matrices, arguing that effective analysis requires combining particle imaging with chemical characterization of the same particles, and highlighting methods capable of simultaneous morphological and chemical identification.
Label-Free Live-Cell Imaging of Internalized Microplastics and Cytoplasmic Organelles with Multicolor CARS Microscopy
Label-free multicolor coherent anti-Stokes Raman scattering (CARS) microscopy was used to simultaneously visualize internalized microplastics and cellular organelles in live cells without requiring fluorescent staining. The approach enables real-time tracking of plastic particle interactions with intracellular structures, offering new insight into how microplastics behave inside human cells.
Imaging Flow Cytometry Protocols for Examining Phagocytosis of Microplastics and Bioparticles by Immune Cells of Aquatic Animals
Imaging flow cytometry was adapted to study how aquatic animal cells take up microplastic particles, enabling detailed, high-throughput analysis of cellular responses to plastic ingestion. This method could help researchers better understand how microplastics harm marine and freshwater organisms at the cellular level.
Quantification of palladium-labelled nanoplastics algal uptake by single cell and single particle inductively coupled plasma mass spectrometry
Researchers developed a method using palladium-labelled nanoplastics and single-cell mass spectrometry to quantify nanoplastic uptake by algal cells. The study demonstrated that this technique can measure nanoplastic exposure on a per-cell basis, providing a valuable new tool for understanding how nanoplastics interact with organisms at the base of aquatic food webs.
Raman Microspectroscopy to Trace the Incorporation of Deuterium from Labeled (Micro)Plastics into Microbial Cells.
Researchers applied stable isotope resonance Raman microspectroscopy at the single-cell level to trace the incorporation of deuterium from labeled (micro)plastics into microbial cells, demonstrating a novel method for tracking microbial degradation of plastics at the cellular level.
Confocal Surface-Enhanced Raman Imaging of the Intestinal Barrier Crossing Behavior of Model Nanoplastics in Daphnia Magna
Using advanced Raman imaging, researchers directly observed nanoplastic particles crossing the intestinal wall of water fleas (Daphnia magna) for the first time. They identified two specific cellular mechanisms — clathrin-dependent endocytosis and macropinocytosis — that cells use to absorb the tiny plastic particles. This study provides concrete evidence of how nanoplastics can pass through the gut barrier into the body, a process relevant to understanding human exposure through food and water.
Identification of Microplastics Using a Custom Built Micro-Raman Spectrometer
Researchers built a custom micro-Raman spectrometer and demonstrated its use for identifying microplastic polymer types in environmental samples, achieving sensitive and specific polymer identification at particle sizes down to a few micrometers.
Fast detection and 3D imaging of nanoplastics and microplastics by stimulated Raman scattering microscopy
Researchers developed a fast imaging technique using stimulated Raman scattering microscopy to detect and create 3D maps of nanoplastics and microplastics at the single-particle level. The method can identify plastic particles as small as 100 nanometers and distinguish between different polymer types without the need for dyes or labels. This technology could help scientists more accurately track tiny plastic particles in environmental and biological samples.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence microscopy, second harmonic generation imaging, and coherent Raman scattering to detect and map micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics were found to cross the blood-brain barrier and accumulate in lipid-rich brain regions in animal models.
Label-free detection of polystyrene nanoparticles in Daphnia magna using Raman confocal mapping
Researchers demonstrated that Raman confocal mapping can detect polystyrene nanoparticles inside Daphnia magna without labels or dyes, revealing particle accumulation in the gut and providing a non-invasive method for studying nanoplastic uptake in organisms.
Morphological and chemical characterization of nanoplastics in human tissue
Researchers developed methods to visualize and chemically characterize nanoplastics that have accumulated in human tissue samples. They were able to identify plastic particles smaller than one micrometer within tissue using advanced microscopy and spectroscopy techniques. The study provides some of the first direct evidence of nanoscale plastic accumulation in the human body, which is essential for designing future health effects research.
Quantitative Analysis of Nanoplastics in Single Cells by Subcellular Chromatography
This study developed a novel subcellular chromatography method capable of quantifying nanoplastic particles in different regions of individual living cells with femtolitre-to-attolitre precision. By directly sampling and separating intracellular cytoplasm, the technique revealed how nanoplastics distribute across different cellular compartments. This advance in analytical capability is important for understanding the subcellular fate of nanoplastics and the spatially specific toxicological mechanisms they may trigger inside cells.
Rapid Single Particle Atmospheric Solids Analysis Probe-Mass Spectrometry for Multimodal Analysis of Microplastics
Researchers developed an atmospheric solid analysis probe coupled to mass spectrometry for rapid chemical characterization of single microplastic particles, enabling polymer identification while remaining compatible with complementary imaging techniques for comprehensive microplastic analysis.
Quantification of Polystyrene Uptake by Different Cell Lines Using Fluorescence Microscopy and Label-Free Visualization of Intracellular Polystyrene Particles by Raman Microspectroscopic Imaging
Scientists tested how human cells take up polystyrene microplastic particles using three cell types that represent the lung lining, intestinal lining, and immune system. All three cell types absorbed the microplastic beads, with immune cells showing different uptake patterns compared to the barrier cells of the lungs and gut. This study confirms that microplastics can enter human cells through multiple exposure routes, including breathing and eating, and that immune cells may play a special role in processing these particles.
Confocal surface-enhanced Raman imaging of the intestine barrier crossing behavior of nanoplastics in Daphnia magna
Using a specially engineered nanoplastic particle visible under confocal Raman imaging, researchers tracked how nanoplastics move from the gut into other organs of the water flea Daphnia magna. The study revealed that nanoplastics can cross the intestinal barrier and translocate to other body parts, providing direct visual evidence of how these particles spread through a living organism and raising concerns about similar processes in other aquatic animals.
Dark-field hyperspectral microscopy for label-free microplastics and nanoplastics detection and identification in vivo: A Caenorhabditis elegans study
Researchers demonstrated that dark-field hyperspectral microscopy can visualize and chemically identify nano- and microplastics (down to 100 nm) in live C. elegans nematodes without labeling, differentiating multiple polymer types simultaneously within intestinal tissue.