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Papers
20 resultsShowing papers similar to A versatile assay platform for enzymatic poly(ethylene-terephthalate) degradation
ClearEnzymatic Degradation of PET plastic
This study tested commercial-grade enzymes for degrading PET plastic and found that enzymatic degradation was effective at laboratory scale but faced challenges for real-world application. Scaling up enzymatic PET recycling could reduce the persistence of plastic waste that eventually fragments into microplastics in the environment.
Recent advances in screening and identification of PET-degrading enzymes
Researchers reviewed recent advances in discovering and engineering enzymes capable of breaking down PET plastic, one of the most widely produced and persistent plastic types. They examined screening methods including metagenomic mining and machine learning approaches that have accelerated the identification of promising PET-degrading enzymes. The study suggests that enzymatic recycling could become a viable, environmentally friendly alternative to traditional PET disposal methods.
Development of Enzyme-Based Approaches for Recycling PET on an Industrial Scale
This paper reviews the development of enzyme-based methods for breaking down PET plastic (used in bottles and packaging) at an industrial scale. While enzymatic recycling is a promising solution to plastic waste, current methods are still too slow and costly for widespread use. Improving these technologies could help reduce the enormous amount of PET entering the environment and breaking down into microplastics.
Marine PET Hydrolase (PET2): Assessment of Terephthalate- and Indole-Based Polyesters Depolymerization
Researchers characterized a marine enzyme (PET2) capable of breaking down PET plastic and related polyester materials under relatively mild conditions. Discovering and engineering enzymes that can degrade PET could help address the massive accumulation of PET microplastics in ocean environments.
A high‐throughput expression and screening platform for applications‐driven PETase engineering
Researchers developed a high-throughput platform for engineering PETase enzymes — which break down plastic polyester — by using secretory expression to eliminate purification steps, enabling faster screening of enzyme variants for industrial plastic biodegradation applications.
Evaluation of enzymatic degradation of petase against PBT and PEN nanoplastics using dye release assay
Researchers expressed, purified, and tested wild-type and engineered variants of PETase enzyme against polyethylene naphthalate and polybutylene terephthalate nanoplastic substrates, developing a dye-release fluorescence assay that correlated with HPLC measurements to enable rapid, quantitative screening of enzymatic plastic degradation.
Process development for PETase production and purification
Researchers developed a production and purification process for PETase, an enzyme capable of breaking down polyethylene terephthalate (PET) plastic biologically, as an alternative to inadequate mechanical and chemical recycling methods for mixed and contaminated PET waste. The study addresses the global plastic pollution crisis by advancing the scalability of enzymatic PET degradation as a sustainable recycling pathway.
Enzymatic PET Degradation
This review examines enzymatic degradation of PET (polyethylene terephthalate), the plastic used in bottles and polyester clothing, as a promising pathway for breaking down this persistent polymer. Advances in engineering more efficient PET-degrading enzymes could enable industrial-scale biological recycling and reduce the environmental accumulation of PET microplastics.
Structural decay of poly(ethylene terephthalate) by enzymatic degradation
Researchers examined the structural decay of poly(ethylene terephthalate) through enzymatic degradation as a sustainable recycling strategy, finding this approach requires neither energy nor harsh solvents, offering a promising path for addressing microplastic pollution from PET products.
An efficient strategy to tailor PET hydrolase: Simple preparation with high yield and enhanced hydrolysis to micro-nano plastics
This study developed a simplified, high-yield preparation method for PET-degrading hydrolase enzymes to improve their ability to break down PET nano- and microplastics. The engineered enzyme showed enhanced hydrolysis activity against PET microplastics, offering a more practical route to enzymatic plastic waste treatment.
Enzymatic Remediation of Polyethylene Terephthalate (PET)–Based Polymers for Effective Management of Plastic Wastes: An Overview
Enzymatic approaches for remediating PET-based plastic waste were reviewed, covering PETase and related enzymes that can break PET into reusable monomers. Enzyme engineering strategies to improve thermostability and catalytic efficiency are discussed as a pathway to scalable biological PET recycling.
Recent advances in enzyme engineering for improved deconstruction of poly(ethylene terephthalate) (PET) plastics
This review covers recent progress in engineering enzymes that can break down PET plastic, the material used in water bottles and food containers. While natural enzymes that digest PET have been discovered, they are not yet fast or durable enough for industrial-scale recycling. Advances in protein engineering, directed evolution, and computational design are steadily improving these enzymes, which could eventually provide a sustainable way to recycle PET and reduce microplastic pollution at its source.
Enhancing PET Degrading Enzymes: A Combinatory Approach
Scientists worked on improving enzymes that can break down PET plastic, one of the most common plastics in consumer products. Using a combinatory approach, researchers enhanced the performance of a naturally occurring PET-degrading enzyme from the bacterium Piscinibacter sakaiensis. The study suggests that engineered enzymes could eventually help create a circular economy for plastic waste by enabling efficient recycling at the molecular level.
An archaeal lid-containing feruloyl-esterase degrades polyethylene terephthalate (PET)
This study identified the first archaeal enzyme capable of degrading PET plastic, characterizing its structure and biochemical properties. Expanding the diversity of organisms with PET-degrading enzymes could accelerate the development of biological strategies for breaking down the microplastics contaminating marine and terrestrial environments.
Marine PET Hydrolase (PET2): Assessment of Terephthalate- and Indole-Based Polyester Depolymerization
This study characterized a marine-derived enzyme (PET2) capable of breaking down PET plastic under mild conditions, assessing its efficiency for enzymatic recycling. Enzyme-based PET recycling could prevent plastic waste from fragmenting into the microplastics that accumulate in oceans and organisms.
Eco-Microbiology: Discovering Biochemical Enhancers of PET Biodegradation by Piscinibacter sakaiensis
This paper reviews biochemical strategies for enhancing PET biodegradation by microorganisms, focusing on the discovery and engineering of plastic-degrading enzymes. The review highlights recent advances and remaining challenges in scaling up enzymatic plastic degradation for industrial applications.
New combined absorption/1H NMR method for qualitative and quantitative analysis of PET degradation products
Researchers developed and validated a combined chemical analysis method using light absorption and NMR spectroscopy to accurately measure the products created when enzymes break down PET plastic — the polymer used in bottles and food containers. Having reliable measurement tools is essential for developing enzyme-based plastic recycling strategies that could convert waste PET back into useful building blocks.
Enzymatic Degradation of Polyethylene Terephthalate Plastics by Bacterial Curli Display PETase
Researchers engineered bacteria to display a PET-degrading enzyme on their surface, creating a reusable biocatalyst capable of breaking down polyethylene terephthalate plastics. The system worked under various conditions, remained stable for at least 30 days, and could even degrade PET microplastics in wastewater and highly crystalline consumer plastic waste. This biological approach offers a promising environmentally friendly alternative for plastic recycling and waste treatment.
Discovery and rational engineering of PET hydrolase with both mesophilic and thermophilic PET hydrolase properties
Researchers discovered a new enzyme from a soil bacterium that can break down PET plastic — the material in most plastic bottles — at both room temperature and elevated heat, then engineered an improved version that degrades PET powder almost completely within half a day at 55°C. This dual-temperature capability makes it more practical than existing enzymes for industrial-scale plastic recycling and could help address the global PET waste problem.
Current knowledge on enzymatic PET degradation and its possible application to waste stream management and other fields
This review distinguished between enzymatic PET surface modification (useful for fiber treatment) and enzymatic PET degradation (needed for waste management), cataloguing the hydrolases capable of each function and the conditions required. The authors evaluate the prospects for deploying PET-degrading enzymes in industrial plastic waste streams.