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Papers
61,005 resultsShowing papers similar to Microfluidic Impedance Cytometry for Single‐Cell Particulate Inorganic Carbon:Particulate Organic Carbon Measurements of Calcifying Algae
ClearCharacterisation of microplastics and unicellular algae in seawater by targeting carbon via single particle and single cell ICP-MS
Researchers used single particle and single cell ICP-MS to characterize microplastics and microalgae simultaneously in seawater by targeting carbon signals, demonstrating the technique as a rapid and sensitive tool for distinguishing plastic particles from biological material.
Discrimination of Microplastics and Phytoplankton Using Impedance Cytometry
Researchers demonstrated that impedance cytometry can discriminate between microplastics and phytoplankton in ocean water samples. The study suggests this technique could enable high-throughput, deployable monitoring of both plankton communities and microplastic pollution levels, addressing a key gap in current marine monitoring capabilities.
Simultaneously Acquiring Optical and Acoustic Properties of Individual Microalgae Cells Suspended in Water
Researchers developed a multimodality technique to simultaneously measure polarized light scattering, fluorescence, and laser-induced acoustic wave signals from individual microalgae cells in water, demonstrating that the combined approach could discriminate between Spirulina species and different physiological states of Microcystis and enable single-cell assessment of photosynthetic energy absorption.
Quantification of palladium-labelled nanoplastics algal uptake by single cell and single particle inductively coupled plasma mass spectrometry
Researchers developed a method using palladium-labelled nanoplastics and single-cell mass spectrometry to quantify nanoplastic uptake by algal cells. The study demonstrated that this technique can measure nanoplastic exposure on a per-cell basis, providing a valuable new tool for understanding how nanoplastics interact with organisms at the base of aquatic food webs.
Opportunities in optical and electrical single-cell technologies to study microbial ecosystems
This paper is not about microplastics; it reviews advanced optical (flow cytometry, Raman spectroscopy) and electrical single-cell analysis technologies used to study microbial communities and ecology.
Inorganic Nanoparticle and Nanoplastic Transformations and the Impact on Biouptake by Freshwater Algal Cells Using Single Cell and Single Particle Inductively Coupled Plasma Mass Spectrometry
This dissertation used single-particle and single-cell ICP-MS to quantify how nanoparticles and nanoplastics transform in the presence of freshwater algae and how those transformations affect biouptake, finding that algal interactions altered particle surface chemistry and changed bioavailability.
A polyp-on-chip for coral long-term culture
Researchers developed a microfluidic chip-based platform for culturing individual coral polyps under controlled conditions, enabling long-term physiological and ecological studies. This tool could be useful for studying how microplastics and chemical pollutants affect coral health at the cellular level.
Determination of carbon in microplastics and single cells by total consumption microdroplet ICP-TOFMS
This study developed a total consumption microdroplet ICP-TOFMS method to determine carbon content in microplastics and single cells, overcoming size limitations of conventional nebulization methods. The approach enables more accurate elemental characterization of individual microplastic particles.
Photonic Microfluidic Technologies for Phytoplankton Research
This review covers photonic microfluidic technologies for studying phytoplankton — microscopic algae that produce half of Earth's oxygen — highlighting how miniaturized optical tools enable single-cell analysis of these ecologically critical organisms.
Effective concentration of marine nanoflagellates with a microfluidic device
Researchers developed a microfluidic device to concentrate marine nanoflagellates — tiny single-celled organisms — without damaging them. The passive, size-based separation method outperformed centrifugation and membrane filtration in preserving cell viability. This technology could improve studies of marine microbial ecology, including how microbes interact with microplastics in ocean environments.
A Droplet-Based Microfluidic Impedance Flow Cytometer for Detection of Micropollutants in Water
A droplet-based microfluidic impedance cytometer was designed and tested for in-situ detection of microplastic particles in water, offering a portable and rapid alternative to laboratory-based analytical methods.
Novel methodology for identification and quantification of microplastics in biological samples
Researchers validated a protocol for identifying and quantifying polyethylene microplastics in biological samples, finding that membrane filtration caused particle retention problems and that flow cytometry offered a more reliable alternative for analysis of biological digests.
Flow cytometry as new promising detection tool for micro and submicron plastic particles
Researchers evaluated flow cytometry as a detection tool for micro- and nanoplastics, testing its ability to rapidly identify and count plastic particles in environmental and biological samples. Results demonstrated that flow cytometry offers a promising high-throughput approach for microplastic detection compared to more time-intensive conventional methods.
In Situ Determination of Chlorella Concentration Using Single Entity Electrochemistry
Researchers developed an electrochemical method for detecting individual algal cells in real time using an ultramicroelectrode and single-particle collision technique. The approach could distinguish individual Chlorella cells and relate collision frequency to algal concentration, offering potential for early detection of harmful algal blooms. While not directly focused on microplastics, the method provides a platform for monitoring water quality impacts related to microplastic-linked eutrophication.
Portable Impedance-Sensing Device for Microorganism Characterization in the Field
This study developed a portable microfluidic device using impedance spectroscopy to rapidly detect and characterize individual microorganisms in heterogeneous field samples. Portable detection technologies are also being applied to monitoring microorganisms associated with microplastic surfaces (the plastisphere) in water.
Imaging Flow Cytometry Protocols for Examining Phagocytosis of Microplastics and Bioparticles by Immune Cells of Aquatic Animals
Imaging flow cytometry was adapted to study how aquatic animal cells take up microplastic particles, enabling detailed, high-throughput analysis of cellular responses to plastic ingestion. This method could help researchers better understand how microplastics harm marine and freshwater organisms at the cellular level.
Dielectrophoretic separation of microalgae cells in ballast water in a microfluidic chip
Researchers developed a microfluidic chip using dielectrophoresis to continuously separate microalgae cells from ballast water, which also contains bacteria, microplastics, and other particles. Improved ballast water treatment is important because ships spread invasive species and contaminants including microplastics to new ocean regions.
Calcium-mediated mitigation of aged nanoplastic-induced stress in microalgae: Insights into photosynthesis, energy metabolism, and antioxidant defense from physiological and multi-omics analyses
Scientists found that tiny plastic particles (nanoplastics) severely damage microalgae, which are important organisms used to clean wastewater before it enters our water supply. However, adding calcium to the water protected the microalgae from this plastic pollution and helped them continue removing harmful substances from wastewater. This research suggests calcium could help maintain clean water treatment systems even as plastic pollution increases in our environment.
Flow cytometry as new promising detection tool for micro and submicron plastic particles
Researchers evaluated flow cytometry as a tool for detecting and counting micro- and submicron plastic particles in environmental and biological samples. The method offered rapid throughput and the ability to distinguish plastic particles from biological material, but required careful optimization for complex matrices.
Study the impact of microplastic pollutants on marine algae by novel dielectric spectroscopy method
Researchers developed a PCB coaxial probe-based dielectric spectroscopy method to assess the impact of microplastic pollutants on marine algae at varying concentrations. By measuring changes in the dielectric constant of algae exposed to microplastics, the study demonstrated that this non-destructive technique can rapidly detect and quantify the effects of microplastics on marine algal physiology.
An Efficient Low-Cost Laboratory Workflow for the Study of Blood Cells and RNA Extractions in Marine Invertebrates
This paper describes a low-cost laboratory method for studying blood cells and extracting genetic material from marine invertebrates, which are widely used as indicators of environmental pollution. Better lab methods will improve our ability to measure the biological effects of microplastic exposure in these organisms.
Technological Advancements in Field Investigations of Marine Microorganisms: From Sampling Strategies to Molecular Analyses
This is not a microplastics study; it reviews advances in field sampling and molecular analysis methods for studying marine microorganisms, covering omics technologies and in-situ sampling strategies for understanding ocean biogeochemical processes.
Pulse Feature-Enhanced Classification of Microalgae and Cyanobacteria Using Polarized Light Scattering and Fluorescence Signals
Researchers used polarization-sensitive flow cytometry with enhanced pulse feature analysis to classify microalgae and cyanobacteria in mixed samples, training classifiers on optical signatures that distinguish cell types without staining. The method achieved high classification accuracy and offers potential for rapid, label-free phytoplankton monitoring in environmental water samples.
Development of a Microfluidic Ion Current Measurement System for Single-Microplastic Detection
Researchers adapted an ion current sensing technique — previously used for single-cell analysis — to measure individual microplastic particles rapidly, achieving size measurements of 100 particles in just two seconds. While the method could not yet distinguish between different polymer types, it opens a path toward high-throughput, single-particle microplastic analysis that could complement slower techniques like Raman spectroscopy and electron microscopy.