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20 resultsShowing papers similar to Label-free stimulated Raman scattering imaging of intracellular microplastics in mammalian cells
ClearLabel-Free Live-Cell Imaging of Internalized Microplastics and Cytoplasmic Organelles with Multicolor CARS Microscopy
Label-free multicolor coherent anti-Stokes Raman scattering (CARS) microscopy was used to simultaneously visualize internalized microplastics and cellular organelles in live cells without requiring fluorescent staining. The approach enables real-time tracking of plastic particle interactions with intracellular structures, offering new insight into how microplastics behave inside human cells.
Localisation and identification of polystyrene particles in tissue sections using Raman spectroscopic imaging
Researchers developed a Raman spectroscopic imaging method to localize and identify polystyrene microplastic particles directly within tissue sections, enabling in-situ detection without fluorescent labeling and making environmental sample analysis feasible.
Raman Microspectroscopy to Trace the Incorporation of Deuterium from Labeled (Micro)Plastics into Microbial Cells.
Researchers applied stable isotope resonance Raman microspectroscopy at the single-cell level to trace the incorporation of deuterium from labeled (micro)plastics into microbial cells, demonstrating a novel method for tracking microbial degradation of plastics at the cellular level.
Quantitative monitoring of microplastics and lipid metabolism in live zebrafish via hyperspectral stimulated Raman scattering microscopy
Researchers used spectral focusing hyperspectral stimulated Raman scattering (SRS) microscopy to longitudinally monitor microplastic uptake, size-dependent organ accumulation, and lipid metabolic changes in live zebrafish during development. They found that microplastic exposure disrupted hepatic lipid metabolism and energy homeostasis, with the SRS imaging approach enabling real-time, label-free tracking of microplastics and associated biochemical changes in living organisms.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence, second harmonic generation, and coherent Raman scattering microscopy in a single instrument to image micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics crossed the blood-brain barrier and accumulated in lipid-rich brain regions in mouse models.
Quantitative assessment and monitoring of microplastics and nanoplastics distributions and lipid metabolism in live zebrafish using hyperspectral stimulated Raman scattering microscopy
Researchers developed a new imaging technique to watch microplastics and nanoplastics accumulate in live zebrafish in real time, without needing dyes or labels. They found that these tiny plastic particles built up in the fish's digestive system and disrupted fat metabolism, providing direct visual evidence of how micro- and nanoplastics can interfere with basic biological processes.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence microscopy, second harmonic generation imaging, and coherent Raman scattering to detect and map micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics were found to cross the blood-brain barrier and accumulate in lipid-rich brain regions in animal models.
Raman-spektroskopische Charakterisierung von Zellen und Gewebe nach Exposition mit Nanoplastik
Researchers exposed human monocytic THP-1 cells, trophoblasts, and placenta cells to primary and secondary nanoplastic particles at 100 particles/cell in sizes of 200 nm and 60 nm, then used confocal laser scanning microscopy and Raman microspectroscopy to locate and characterize intracellular nanoplastics.
Imaging and quantifying the biological uptake and distribution of nanoplastics using a dual-functional model material
Researchers developed a dual-functional nanoplastic model material that allows both imaging and precise quantification of nanoplastic uptake in biological systems. Using surface-enhanced Raman spectroscopy and inductively coupled plasma mass spectrometry, they could track where nanoplastics accumulated in organisms at high resolution. The tool addresses a major gap in nanoplastic research by enabling more accurate measurement of how these tiny particles interact with living tissues.
Fast microplastics identification with stimulated Raman scattering microscopy
Stimulated Raman scattering microscopy was applied to rapidly identify and image microplastic particles in complex environmental samples at speeds dramatically faster than conventional Raman spectroscopy. The technique has potential to enable high-throughput microplastic analysis that could make large-scale environmental monitoring more feasible.
Label-free human-disease characterization through circulating cell free DNA analysis using Raman Spectroscopy
Not relevant to microplastics — this study uses Raman spectroscopy to analyse cell-free DNA in blood as a diagnostic tool for cancer and diabetes, with no connection to microplastic research.
Fast detection and 3D imaging of nanoplastics and microplastics by stimulated Raman scattering microscopy
Researchers developed a fast imaging technique using stimulated Raman scattering microscopy to detect and create 3D maps of nanoplastics and microplastics at the single-particle level. The method can identify plastic particles as small as 100 nanometers and distinguish between different polymer types without the need for dyes or labels. This technology could help scientists more accurately track tiny plastic particles in environmental and biological samples.
Limits of the detection of microplastics in fish tissue using stimulated Raman scattering microscopy
This study demonstrated the detection sensitivity of stimulated Raman scattering microscopy for identifying microplastic beads within fish tissue, characterizing how signal-to-noise ratio varies with particle size. The technique provided chemical contrast to distinguish different plastic types within biological tissue without destructive sample preparation.
Stimulated Raman scattering simulation for imaging optimization
This paper describes simulation tools for optimizing stimulated Raman scattering microscopy, a technique used for rapid, label-free imaging. SRS microscopy is one of the emerging methods being developed to detect and characterize microplastics in complex environmental and biological samples.
Label-free detection of polystyrene nanoparticles in Daphnia magna using Raman confocal mapping
Researchers demonstrated that Raman confocal mapping can detect polystyrene nanoparticles inside Daphnia magna without labels or dyes, revealing particle accumulation in the gut and providing a non-invasive method for studying nanoplastic uptake in organisms.
Advancements in optical techniques for direct identification and localization of micro- and nanoplastics in biological samples
Researchers reviewed advanced optical methods for directly detecting and localizing microplastics in biological tissues, highlighting techniques that can identify particles without extraction or digestion. Optical approaches including Raman mapping and coherent anti-Stokes Raman scattering allow spatial mapping of microplastics in tissue sections.
Raman Spectroscopic Imaging of Human Bladder Resectates towards Intraoperative Cancer Assessment
Researchers used Raman spectroscopy imaging to distinguish between healthy and cancerous human bladder tissue without the need for chemical stains or labels. The technique successfully identified cancer regions in tissue samples from ten patients, using advanced data analysis to map molecular differences. While not directly related to microplastics, this spectroscopy method is also used in microplastic research and demonstrates the power of label-free chemical imaging in medical applications.
Raman Spectroscopic Detection of Silicone Leakage in Human Breast and Lymph Node Tissues
This paper is not relevant to microplastics; it uses Raman spectroscopy to detect silicone leakage from ruptured breast implants in human tissue samples.
Quantification of Polystyrene Uptake by Different Cell Lines Using Fluorescence Microscopy and Label-Free Visualization of Intracellular Polystyrene Particles by Raman Microspectroscopic Imaging
Scientists tested how human cells take up polystyrene microplastic particles using three cell types that represent the lung lining, intestinal lining, and immune system. All three cell types absorbed the microplastic beads, with immune cells showing different uptake patterns compared to the barrier cells of the lungs and gut. This study confirms that microplastics can enter human cells through multiple exposure routes, including breathing and eating, and that immune cells may play a special role in processing these particles.
How to Identify and Quantify Microplastics and Nanoplastics Using Raman Imaging?
This paper reviews advances in Raman imaging as a method for identifying and quantifying microplastics and nanoplastics in environmental samples, discussing current protocols, analytical challenges, and the need for standardization.