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61,005 resultsShowing papers similar to Daphnia magna uptake and excretion of luminescence‐labelled polystyrene nanoparticle as visualized by high sensitivity real-time optical imaging
ClearConfocal Surface-enhanced Raman Imaging of the Intestine Barrier Crossing Behavior of Dual-functional Plasmonic Nanoplastics in Daphnia magna
Using gold-coated polystyrene nanoplastics as dual-function probes, researchers tracked how nanoplastics move through the body of the water flea Daphnia magna after ingestion, observing that particles initially accumulate in the intestine and then translocate to other organs within four hours at environmentally concerning concentrations. This direct visualization of inter-organ translocation in a key aquatic model organism strengthens concerns that nanoplastic pollution can spread beyond the gut and affect multiple body systems.
Ingestion of micro- and nanoplastics in Daphnia magna – Quantification of body burdens and assessment of feeding rates and reproduction
Researchers used a quantitative approach to measure how the water flea Daphnia magna ingests and excretes micro- and nanoplastic particles of different sizes. They found that larger 2-micrometer particles were ingested in greater mass than 100-nanometer particles, and that complete excretion did not occur within 24 hours. Chronic exposure reduced feeding rates and reproduction, suggesting that ongoing microplastic exposure could have meaningful ecological consequences for these important freshwater organisms.
Confocal surface-enhanced Raman imaging of the intestine barrier crossing behavior of nanoplastics in Daphnia magna
Using a specially engineered nanoplastic particle visible under confocal Raman imaging, researchers tracked how nanoplastics move from the gut into other organs of the water flea Daphnia magna. The study revealed that nanoplastics can cross the intestinal barrier and translocate to other body parts, providing direct visual evidence of how these particles spread through a living organism and raising concerns about similar processes in other aquatic animals.
When Fluorescence Is not a Particle: The Tissue Translocation of Microplastics in Daphnia magna Seems an Artifact
Researchers investigated whether fluorescent polystyrene microplastic beads truly translocate from the gut to body tissues in the water flea Daphnia magna, as previous studies had reported. Using confocal microscopy and passive sampling, they found that the fluorescent dye leached off the plastic beads and accumulated in lipid droplets independently of the particles themselves. The study demonstrates that using fluorescence as a proxy for tracking microplastics can produce misleading results about tissue uptake.
Molecular, biochemical and behavioral responses of Daphnia magna under long-term exposure to polystyrene nanoplastics
Researchers studied the long-term effects of polystyrene nanoplastics on the water flea Daphnia magna over a 21-day exposure period at environmentally relevant concentrations. The study found molecular, biochemical, and behavioral changes even at low concentrations, suggesting that chronic exposure to nanoplastics may have significant impacts on aquatic organisms that short-term studies might miss.
Accumulation kinetics of polystyrene nano- and microplastics in the waterflea Daphnia magna and trophic transfer to the mysid Limnomysis benedeni
Researchers investigated the accumulation kinetics of polystyrene particles ranging from 26 nm to 4800 nm in Daphnia magna and their subsequent transfer to the mysid Limnomysis benedeni. Smaller particles accumulated more efficiently in Daphnia, and trophic transfer to mysids was demonstrated, confirming that nano- and microplastics move through aquatic food chains with size-dependent efficiency.
Mass spectrometry imaging enables detection of MPs and their effects in Daphnia magna following acute exposure
Researchers used an advanced imaging technique called mass spectrometry imaging to track where microplastics accumulate inside water fleas after short-term exposure. They found that the tiny organisms ingested microplastics that concentrated in their gut, and the exposure altered their lipid metabolism. The technique offers a new way to visualize exactly where microplastics end up in small aquatic organisms and what biochemical changes they cause.
Confocal Surface-enhanced Raman Imaging of the Intestine Barrier Crossing Behavior of Dual-functional Plasmonic Nanoplastics in Daphnia magna
Scientists used gold-coated polystyrene nanoplastics as SERS (surface-enhanced Raman scattering) probes to track — at high spatial resolution — exactly how nanoplastics move from the gut of the water flea Daphnia magna into other organs. After four hours of exposure at 10 mg/L, nanoplastics began crossing from the intestine to other body compartments, confirming that translocation — not just ingestion — occurs even in a tiny invertebrate. This matters because Daphnia are a cornerstone of freshwater food webs, and organ-level accumulation could affect their survival and the organisms that eat them.
Selective ingestion and response by Daphnia magna to environmental challenges of microplastics
Researchers used fluorescent microplastics labeled with aggregation-induced emission markers to investigate how Daphnia magna selectively ingests different types of plastic particles, finding that particle type, size, and surface chemistry influence ingestion patterns and toxicological response.
Quantifying nanoplastic-bound chemicals accumulated in Daphnia magna with a passive dosing method
A passive dosing method was used to measure how chemicals accumulate in Daphnia water fleas when nanoplastics are present, helping separate direct particle effects from chemical effects. Understanding which pathway causes more harm is essential for accurately assessing nanoplastic risk.
Potential for high toxicity of polystyrene nanoplastics to the European Daphnia longispina
Researchers found that polystyrene nanoplastics caused high toxicity in three genetically distinct clones of the European water flea Daphnia longispina, highlighting the ecological hazard of nanoplastics and the importance of reporting exposure in particle count rather than mass metrics.
Polystyrene nanoplastics inhibit reproduction and induce abnormal embryonic development in the freshwater crustacean Daphnia galeata
Researchers exposed the freshwater crustacean Daphnia galeata to polystyrene nanoparticles and observed significant decreases in survival, reproduction, and embryonic development. Using fluorescence microscopy, they tracked the particles as they transferred from external body surfaces to internal organs including the ovaries and brood chamber. The study also found that exposed adults had fewer and smaller lipid droplets, suggesting that nanoplastics disrupt energy storage and reproductive capacity in these organisms.
Label-free detection of polystyrene nanoparticles in Daphnia magna using Raman confocal mapping
Researchers demonstrated that Raman confocal mapping can detect polystyrene nanoparticles inside Daphnia magna without labels or dyes, revealing particle accumulation in the gut and providing a non-invasive method for studying nanoplastic uptake in organisms.
Luminous Upconverted Nanoparticles as High-Sensitivity Optical Probes for Visualizing Nano- and Microplastics in Caenorhabditis elegans
Researchers used upconverted NaYF4:Yb3+/Er3+ nanoparticles as high-sensitivity optical probes to visualize the ingestion and biodistribution of polystyrene microplastics and nanoplastics in Caenorhabditis elegans in real time, overcoming autofluorescence limitations of conventional fluorescent probes.
Tracking Nano- and Microplastics Accumulation and Egestion in a Marine Copepod by Novel Fluorescent AIEgens: Kinetic Modeling of the Rhythm Behavior
Researchers used advanced fluorescent probes to precisely track how a marine copepod species takes in and expels nano- and microplastic particles of different sizes and surface types. They found that the copepods followed rhythmic feeding and excretion patterns, with smaller nanoplastics accumulating more readily and being expelled more slowly than larger particles. The study provides detailed data on how tiny marine organisms process plastic particles, which is important for understanding how plastics move through ocean food webs.
Tissue translocation, multigenerational and population effects of microplastics in Daphnia magna
This study examined how microplastics are taken up by the water flea Daphnia magna and whether exposure causes multigenerational effects, finding that microplastics can accumulate in body tissues and pass to offspring. The results suggest microplastic pollution poses risks not just to exposed individuals but can affect population health across generations.
Fluorescent carbon dot embedded polystyrene nanoplastic: In vivo toxicity assessment and bioaccumulation study in Daphnia magna
Researchers synthesized fluorescent carbon dot-embedded polystyrene nanoplastics as trackable model particles and used them to assess in vivo toxicity in a biological test system. The fluorescent particles enabled real-time visualization of nanoplastic distribution and associated cellular toxicity.
Ingestion and Egestion of Microplastics by the Cladoceran Daphnia magna: Effects of Regular and Irregular Shaped Plastic and Sorbed Phenanthrene
Researchers studied how the water flea Daphnia magna ingests and excretes polyethylene microplastics of different shapes and sizes. They found that irregular-shaped fragments were ingested differently than regular beads, and that neither type caused acute mortality at tested concentrations — but the study raises questions about chronic effects in this key freshwater species.
Toxicological Evaluation and Quantification of Ingested Metal-Core Nanoplastic by Daphnia magna Through Fluorescence and Inductively Coupled Plasma-Mass Spectrometric Methods
Researchers developed a method using both fluorescence microscopy and ICP-MS to simultaneously quantify nanoplastic particles ingested by Daphnia magna and assess associated toxicity, finding dose-dependent uptake and toxic effects. The combined quantification and toxicity approach provides a more complete picture of nanoplastic risk to freshwater zooplankton than either method alone.
Sublethal impacts of fragmented polyethylene nanoplastics on Daphnia magna following chronic exposure
Researchers exposed Daphnia magna (water fleas) to fragmented polyethylene nanoplastics over a chronic period and observed adverse sublethal effects. The study suggests that even at concentrations that do not cause outright mortality, fragmented nanoplastics from real-world polyethylene degradation can impair the health and function of these important freshwater organisms.
Uptake, bioaccumulation, biodistribution and depuration of polystyrene nanoplastics in zebrafish (Danio rerio)
Researchers used advanced mass spectrometry to track how polystyrene nanoplastics accumulate in and are cleared from zebrafish tissues over time. The nanoplastics concentrated most in the intestine, liver, and gills, with only partial clearance after the exposure ended. This study provides important data on how persistent nanoplastics can be in living organisms, which helps scientists better assess the long-term risks of plastic particle exposure.
Evaluating sublethal effects of long-term exposure of Daphnia magna to nanoplastics at a low concentration
Lab experiments exposed Daphnia magna — a water flea that links primary producers to larger predators — to nanoplastics at low concentrations over multiple generations. The nanoplastics caused sublethal reproductive effects that became more pronounced over successive generations, suggesting that long-term, low-level nanoplastic exposure in the environment could gradually impair aquatic invertebrate population health.
Effects of polystyrene in the brackish water flea Diaphanosoma celebensis: Size-dependent acute toxicity, ingestion, egestion, and antioxidant response
Researchers investigated the effects of different-sized polystyrene beads on the brackish water flea Diaphanosoma celebensis, examining ingestion, tissue distribution, and antioxidant responses. While none of the particle sizes caused mortality, the smallest nano-sized beads (0.05 micrometers) were widely distributed throughout the body including embryos and induced lipid damage. The findings suggest that smaller microplastics penetrate deeper into tissues and cause greater oxidative stress than larger particles.
Potential for high toxicity of polystyrene nanoplastics to the European Daphnia longispina
Researchers exposed water fleas (Daphnia) to polystyrene nanoplastics and found that 50 nm particles were thousands of times more toxic per unit mass than 100 nm particles, with effects comparable to highly regulated toxic chemicals. The results highlight how particle size dramatically changes nanoplastic hazard and challenge the assumption that microplastics pose low ecological risk.