Papers

61,005 results
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Article Tier 2

Microbial enzymes for the recycling of recalcitrant petroleum‐based plastics: how far are we?

This review examines the progress in identifying microbial enzymes capable of breaking down petroleum-based plastics like polyethylene, polystyrene, polyurethane, and PET. Researchers highlight recent advances in using polyester-degrading enzymes to recover raw materials from PET waste through biocatalytic recycling. The study discusses the potential and remaining challenges of using biological approaches to address the growing global problem of plastic waste accumulation.

2017 Microbial Biotechnology 788 citations
Article Tier 2

Enzymatic Remediation of Polyethylene Terephthalate (PET)–Based Polymers for Effective Management of Plastic Wastes: An Overview

Enzymatic approaches for remediating PET-based plastic waste were reviewed, covering PETase and related enzymes that can break PET into reusable monomers. Enzyme engineering strategies to improve thermostability and catalytic efficiency are discussed as a pathway to scalable biological PET recycling.

2020 Frontiers in Bioengineering and Biotechnology 193 citations
Article Tier 2

An Overview into Polyethylene Terephthalate (PET) Hydrolases and Efforts in Tailoring Enzymes for Improved Plastic Degradation

This review examines the discovery and engineering of PET-degrading enzymes including PETase and cutinase variants, discussing protein engineering strategies to improve catalytic efficiency and thermostability for practical biodegradation of polyethylene terephthalate plastic waste.

2022 International Journal of Molecular Sciences 120 citations
Article Tier 2

Characterization and engineering of a plastic-degrading aromatic polyesterase

Researchers characterized and engineered an aromatic polyesterase enzyme capable of degrading plastic polymers, improving its activity through protein engineering and demonstrating its potential as a tool for biodegradation-based plastic cleanup.

2018 Proceedings of the National Academy of Sciences 1006 citations
Article Tier 2

Enzyme-Assisted Depolymerization of Polymers

This review examines enzyme-assisted depolymerization of both synthetic plastics (PET, PLA) and biomass polymers (polysaccharides, lignin), covering the mechanisms and application potential of lipases, esterases, cellulases, lignin peroxidases, and chitosanase. The authors highlight advances in enzyme engineering to enhance stability and activity, particularly for microplastic degradation, and discuss the industrial prospects of enzymatic recycling.

2025 ACS symposium series
Article Tier 2

Enzymatic PET Degradation

This review examines enzymatic degradation of PET (polyethylene terephthalate), the plastic used in bottles and polyester clothing, as a promising pathway for breaking down this persistent polymer. Advances in engineering more efficient PET-degrading enzymes could enable industrial-scale biological recycling and reduce the environmental accumulation of PET microplastics.

2019 CHIMIA International Journal for Chemistry 50 citations
Article Tier 2

Current knowledge on enzymatic PET degradation and its possible application to waste stream management and other fields

This review distinguished between enzymatic PET surface modification (useful for fiber treatment) and enzymatic PET degradation (needed for waste management), cataloguing the hydrolases capable of each function and the conditions required. The authors evaluate the prospects for deploying PET-degrading enzymes in industrial plastic waste streams.

2019 Applied Microbiology and Biotechnology 576 citations
Article Tier 2

Eco-Microbiology: Discovering Biochemical Enhancers of PET Biodegradation by Piscinibacter sakaiensis

This paper reviews biochemical strategies for enhancing PET biodegradation by microorganisms, focusing on the discovery and engineering of plastic-degrading enzymes. The review highlights recent advances and remaining challenges in scaling up enzymatic plastic degradation for industrial applications.

2024
Article Tier 2

Microbial engineering strategies for synthetic microplastics clean up: A review on recent approaches

This review examined microbial engineering strategies for breaking down synthetic microplastics, covering PETase and MHETase enzyme engineering, immobilization approaches, and the major challenges that remain before biological plastic cleanup can be deployed at scale.

2022 Environmental Toxicology and Pharmacology 36 citations
Article Tier 2

An efficient strategy to tailor PET hydrolase: Simple preparation with high yield and enhanced hydrolysis to micro-nano plastics

This study developed a simplified, high-yield preparation method for PET-degrading hydrolase enzymes to improve their ability to break down PET nano- and microplastics. The engineered enzyme showed enhanced hydrolysis activity against PET microplastics, offering a more practical route to enzymatic plastic waste treatment.

2024 International Journal of Biological Macromolecules 5 citations
Article Tier 2

Recent trends in microbial and enzymatic plastic degradation: a solution for plastic pollution predicaments

This review covers recent advances in using microorganisms and their enzymes to break down plastics including polyethylene, PVC, polystyrene, and PET, with techniques like protein engineering being used to boost enzyme efficiency. Microbial degradation offers a sustainable approach to reducing the persistent plastic pollution that generates the microplastics found throughout the environment and human body.

2024 Biotechnology for Sustainable Materials 64 citations
Article Tier 2

Engineered polyethylene terephthalate hydrolases: perspectives and limits

This review examines progress in engineering enzymes that can break down PET plastic, the material used in most beverage bottles and synthetic textiles. Researchers found that while significant advances have been made through protein engineering and machine learning, no enzyme yet exists that can efficiently degrade the crystalline form of PET found in real-world waste. The study outlines the key challenges remaining before enzymatic plastic recycling can work at industrial scale, including handling microplastic contamination.

2024 Applied Microbiology and Biotechnology 27 citations
Article Tier 2

Marine PET Hydrolase (PET2): Assessment of Terephthalate- and Indole-Based Polyesters Depolymerization

Researchers characterized a marine enzyme (PET2) capable of breaking down PET plastic and related polyester materials under relatively mild conditions. Discovering and engineering enzymes that can degrade PET could help address the massive accumulation of PET microplastics in ocean environments.

2023 Preprints.org 1 citations
Article Tier 2

The Current State of Research on PET Hydrolyzing Enzymes Available for Biorecycling

This review summarizes the current state of PET-hydrolyzing enzymes, including thermophilic cutinases and engineered variants, that are candidates for enzymatic biorecycling of PET plastic waste back into reusable monomers.

2021 Catalysts 91 citations
Article Tier 2

Characterization and engineering of plastic-degrading polyesterases jmPE13 and jmPE14 from Pseudomonas bacterium

Two new plastic-degrading polyesterases, jmPE13 and jmPE14, were characterized and engineered from Pseudomonas strains to improve their efficiency in hydrolyzing polyester plastics. The study aimed to develop higher-performance biocatalysts for the enzymatic upcycling of plastic waste.

2024 Frontiers in Bioengineering and Biotechnology 8 citations
Article Tier 2

Biodegradation of Plastic and the Role of Microbial Enzymes in Plastic Waste Management

This review examines how microbial enzymes, particularly PET hydrolases and oxidative enzymes, can depolymerize and break down common plastic polymers through biological degradation. The study suggests that enzymatic approaches to plastic waste management offer a promising complement to mechanical and chemical recycling, though optimizing enzyme activity and scaling up the process remain key challenges.

2026 International Journal of Education Management and Technology
Article Tier 2

Microbial plastic degradation: enzymes, pathways, challenges, and perspectives.

This review synthesizes current knowledge on microbial plastic degradation, covering the enzymes and metabolic pathways involved in breaking down major synthetic polymers, the challenges limiting efficient biodegradation, and perspectives for engineering improved microbial solutions to plastic waste.

2025 Microbiology and molecular biology reviews : MMBR
Article Tier 2

Recent advances in enzyme engineering for improved deconstruction of poly(ethylene terephthalate) (PET) plastics

This review covers recent progress in engineering enzymes that can break down PET plastic, the material used in water bottles and food containers. While natural enzymes that digest PET have been discovered, they are not yet fast or durable enough for industrial-scale recycling. Advances in protein engineering, directed evolution, and computational design are steadily improving these enzymes, which could eventually provide a sustainable way to recycle PET and reduce microplastic pollution at its source.

2025 Communications Materials 7 citations
Article Tier 2

Discovery and Biochemical Characterization of a Novel Polyesterase for the Degradation of Synthetic Plastics

Researchers used bioinformatics to discover a new enzyme from soil bacteria capable of breaking down synthetic plastics like PET and polyurethane. The enzyme was successfully expressed and characterized in the lab, offering a promising lead for developing biological plastic recycling approaches.

2020 3 citations
Article Tier 2

A review on microbial bioremediation of polyethylene terephthalate microplastics

This review focuses on microbial biodegradation of PET microplastics — the plastic used in bottles and synthetic textiles — detailing the specific enzymes (PETase and MHETase) that bacteria use to break the polymer down into its chemical building blocks. Biological degradation offers a lower-energy, more environmentally gentle alternative to chemical recycling or landfill, and understanding the microbial mechanisms involved is key to developing scalable bioremediation solutions for one of the most pervasive microplastic types.

2024 Environmental Quality Management 2 citations
Article Tier 2

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET

This review discusses the role of enzymatic biocatalysis in PET plastic degradation, examining how the discovery of PETase and subsequent enzyme engineering have advanced biodegradation as an alternative to chemical and mechanical recycling for one of the most produced plastics globally.

2021 International Journal of Molecular Sciences 97 citations
Article Tier 2

Plastic biodegradation: Frontline microbes and their enzymes

Researchers reviewed microbial biodegradation of synthetic plastics — including PE, PP, PS, and PET — cataloguing the insects, bacteria, and fungi capable of breaking down these polymers along with the enzymatic mechanisms involved, and outlining paths forward including metabolic pathway engineering and molecular cloning to improve degradation rates.

2020 The Science of The Total Environment 663 citations
Article Tier 2

Bioprospecting for polyesterase activity relevant for PET degradation in marine Enterobacterales isolates

Researchers screened marine Enterobacterales isolates for polyesterase activity capable of degrading PET plastic, identifying bacterial strains from marine environments as candidates for bioremediation strategies targeting one of the world's most problematic plastic pollutants.

2023 AIMS Microbiology 8 citations
Article Tier 2

An archaeal lid-containing feruloyl-esterase degrades polyethylene terephthalate (PET)

This study identified the first archaeal enzyme capable of degrading PET plastic, characterizing its structure and biochemical properties. Expanding the diversity of organisms with PET-degrading enzymes could accelerate the development of biological strategies for breaking down the microplastics contaminating marine and terrestrial environments.

2023