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Papers
61,005 resultsShowing papers similar to Limits of the detection of microplastics in fish tissue using stimulated Raman scattering microscopy
ClearMolecular identification of polymers and anthropogenic particles extracted from oceanic water and fish stomach – A Raman micro-spectroscopy study
Researchers applied Raman micro-spectroscopy to identify plastic polymers in Pacific Ocean trawl samples and fish stomach contents, finding polyethylene and polypropylene as the dominant types while demonstrating that many particles visually suspected as plastic were actually non-polymer biological material — underscoring the necessity of chemical verification.
Optimization of a protocol for the extraction and chemical characterization of microplastics in Chamelea gallina by Raman microspectroscopy
This study optimized a protocol for extracting and chemically characterizing microplastics in the striped venus clam (Chamelea gallina) using Raman microspectroscopy, addressing the challenge of analyzing microplastics in complex biological matrices for food safety and environmental monitoring.
Label-free stimulated Raman scattering imaging of intracellular microplastics in mammalian cells
Researchers used label-free stimulated Raman scattering imaging to visualize microplastic uptake and distribution inside mammalian cells without fluorescent labels, finding that intracellular microplastics were associated with elevated reactive oxygen species, reduced cell viability, and altered lipid metabolism.
Misinterpretation in microplastic detection in biological tissues: When 2D imaging is not enough
Researchers demonstrated that 2D Raman imaging alone can misidentify microplastics in biological tissues, showing that 3D confocal Raman imaging is necessary to accurately distinguish microplastic particles from tissue components in mussels.
Detection of Anthropogenic Particles in Fish Stomachs: An Isolation Method Adapted to Identification by Raman Spectroscopy
This study developed a method for separating anthropogenic particles — including microplastics — from bulk fish stomach contents using an adapted protocol, improving the efficiency of visual examination. The approach aims to reduce misidentification errors that occur when relying solely on color, size, and shape to distinguish plastic from organic matter.
Microplastics in eviscerated flesh and excised organs of dried fish
This study detected microplastics in both the eviscerated flesh and excised organs of four commonly consumed dried fish species, using Raman spectroscopy for polymer identification. The finding raises food safety concerns because it suggests microplastics can be present even in the edible portions of commercially sold fish.
Localisation and identification of polystyrene particles in tissue sections using Raman spectroscopic imaging
Researchers developed a Raman spectroscopic imaging method to localize and identify polystyrene microplastic particles directly within tissue sections, enabling in-situ detection without fluorescent labeling and making environmental sample analysis feasible.
Advancements in optical techniques for direct identification and localization of micro- and nanoplastics in biological samples
Researchers reviewed advanced optical methods for directly detecting and localizing microplastics in biological tissues, highlighting techniques that can identify particles without extraction or digestion. Optical approaches including Raman mapping and coherent anti-Stokes Raman scattering allow spatial mapping of microplastics in tissue sections.
Quantitative assessment and monitoring of microplastics and nanoplastics distributions and lipid metabolism in live zebrafish using hyperspectral stimulated Raman scattering microscopy
Researchers developed a new imaging technique to watch microplastics and nanoplastics accumulate in live zebrafish in real time, without needing dyes or labels. They found that these tiny plastic particles built up in the fish's digestive system and disrupted fat metabolism, providing direct visual evidence of how micro- and nanoplastics can interfere with basic biological processes.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence, second harmonic generation, and coherent Raman scattering microscopy in a single instrument to image micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics crossed the blood-brain barrier and accumulated in lipid-rich brain regions in mouse models.
Quantitative analysis of microplastics in water by Raman spectroscopy: influence of microplastic concentration on Raman scattering intensities
Researchers investigated quantitative Raman spectroscopy for detecting microplastics directly in water, finding that Raman scattering intensities varied with concentration for both PVC spheres (40-100 um) and PE spheres (40-48 um) dispersed in de-ionized water at 0.1-1.0 wt%.
Estimation of contamination level in microplastic-exposed crayfish by laser confocal micro-Raman imaging
Researchers exposed crayfish to varying microplastic concentrations and used laser confocal micro-Raman imaging to detect contamination in muscle and gill tissues, finding that microplastic accumulation increased with exposure concentration and duration.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence microscopy, second harmonic generation imaging, and coherent Raman scattering to detect and map micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics were found to cross the blood-brain barrier and accumulate in lipid-rich brain regions in animal models.
Fast microplastics identification with stimulated Raman scattering microscopy
Stimulated Raman scattering microscopy was applied to rapidly identify and image microplastic particles in complex environmental samples at speeds dramatically faster than conventional Raman spectroscopy. The technique has potential to enable high-throughput microplastic analysis that could make large-scale environmental monitoring more feasible.
Raman Spectroscopy for the Analysis of Microplastics in Aquatic Systems
This review outlined the current status of Raman spectroscopy for analyzing microplastics in aquatic systems, highlighting its high spatial resolution advantage for detecting small particles while critically assessing its drawbacks and best practices for effective use.
Distinguishing Microplastics from Microplastic-like particles: a Case study of Fish from the Marine Waters of Qatar
Researchers examined microplastic accumulation in the gastrointestinal tracts of 170 fish from four commercially important species in Qatar's marine waters, finding that fibers were the dominant shape and blue the most common color, but micro-Raman spectroscopy confirmed only 7 particles (4.12% of samples) met the polymer-match threshold to be classified as true microplastics. The study underscores the importance of chemical confirmation methods to distinguish genuine microplastics from morphologically similar natural particles.
Optimization of a protocol for the extraction and chemical characterization of microplastics in Chamelea gallina by Raman microspectroscopy
Researchers optimized an extraction and chemical characterization protocol for microplastics in bivalve molluscs, improving polymer identification through combined spectroscopic methods. The validated protocol provides a reliable approach for routine microplastic monitoring in shellfish used as bioindicators.
Identification and visualisation of microplastics/ nanoplastics by Raman imaging (ii): Smaller than the diffraction limit of laser?
Researchers examined whether confocal Raman microscopy can identify and visualize nanoplastics smaller than the diffraction limit of the laser, analyzing the lateral intensity distribution of Raman signals from nanoplastics ranging from approximately 30 to 600 nm in diameter. The study found that while imaging resolution is limited by diffraction, chemical identification of sub-diffraction-limit nanoplastics remains possible.
Detection of microplastics and nanoplastics: Are Raman tweezers and enhanced Raman methods the solution for sub 20 μm particles?
Raman tweezers — devices that use a laser beam to trap and analyze individual particles — combined with plasmonic enhancement techniques can detect and characterize nanoplastics and microplastics smaller than 20 µm, a size range that defeats most conventional filtration-based detection methods. Improving detection sensitivity for the smallest plastic particles is critical because nanoplastics are thought to be the most biologically active fraction, capable of crossing cell membranes and accumulating in tissues.
Quantitative monitoring of microplastics and lipid metabolism in live zebrafish via hyperspectral stimulated Raman scattering microscopy
Researchers used spectral focusing hyperspectral stimulated Raman scattering (SRS) microscopy to longitudinally monitor microplastic uptake, size-dependent organ accumulation, and lipid metabolic changes in live zebrafish during development. They found that microplastic exposure disrupted hepatic lipid metabolism and energy homeostasis, with the SRS imaging approach enabling real-time, label-free tracking of microplastics and associated biochemical changes in living organisms.
Identification of Microplastics Using a Custom Built Micro-Raman Spectrometer
Researchers built a custom micro-Raman spectrometer and demonstrated its use for identifying microplastic polymer types in environmental samples, achieving sensitive and specific polymer identification at particle sizes down to a few micrometers.
How to Identify and Quantify Microplastics and Nanoplastics Using Raman Imaging?
This paper reviews advances in Raman imaging as a method for identifying and quantifying microplastics and nanoplastics in environmental samples, discussing current protocols, analytical challenges, and the need for standardization.
A review of recent progress in the application of Raman spectroscopy and SERS detection of microplastics and derivatives
This review covers advances in using Raman spectroscopy and surface-enhanced Raman spectroscopy (SERS) to detect and identify microplastics in the environment. These techniques offer high resolution and sensitive detection that can identify specific plastic types even at very small sizes. Better detection methods are essential for understanding the true extent of microplastic contamination and its potential risks to human health.
Study on Rapid Recognition of Marine Microplastics Based on Raman Spectroscopy
Researchers developed a rapid identification system for marine microplastics using Raman spectroscopy, enabling quick determination of plastic type and size. Fast, accurate identification tools are critical for monitoring the growing problem of microplastic pollution in ocean environments.