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61,005 resultsShowing papers similar to Microplastic extraction from digestive tracts of large decapods
ClearA review of current approaches for the study of microplastic contamination in crustaceans
This review assessed current methodological approaches for studying microplastic contamination in crustaceans, evaluating techniques for extraction, identification, and quantification while highlighting gaps in standardization that limit cross-study comparisons and risk assessment for these ecologically important invertebrates.
Evaluation and optimisation of sample preparation protocols suitable for the analysis of plastic particles present in seafood
Ten digestion protocols for extracting microplastics from seafood were compared across a broad range of species, evaluating their ability to fully digest tissue without damaging polymer integrity, providing a basis for selecting suitable methods for human exposure assessment.
Microplastic pollution in wild populations of decapod crustaceans: A review
Researchers reviewed the occurrence and characteristics of microplastics found in wild populations of decapod crustaceans including crabs and shrimps. They found that fibrous microplastics smaller than 1 mm were the most commonly detected type, and that edible portions generally contained fewer microplastics than non-edible parts. The review highlights the importance of understanding microplastic contamination in commercially valuable crustaceans for both ecological and food safety assessments.
Development and optimization of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues
Researchers optimized methods for digesting mussel soft tissue and extracting microplastics for analysis, finding that sodium hydroxide and enzymatic digestion both achieved high recoveries (~93%) while acid digestion damaged certain plastic types. The validated method provides a reliable protocol for monitoring microplastic contamination in commercially important bivalves.
Development and Validation of an Efficient Method for Processing Microplastics in Biota Samples
A new one-step laboratory method was developed to efficiently digest and extract microplastics from mussel and fish tissue samples. Standardized extraction methods are critical for making microplastic studies comparable across different labs and species.
Extraction and identification of microplastics from mussels: Method development and preliminary results
Scientists developed and validated a method for extracting and identifying microplastics from mussel tissue, then applied it to measure contamination in commercially harvested mussels. The method produced reliable, reproducible results, providing a practical tool for monitoring microplastic levels in one of the world's most widely consumed shellfish.
Microplastics in seafood: Benchmark protocol for their extraction and characterization
Researchers developed a benchmark protocol for extracting and characterizing microplastics from seafood samples, providing standardized methods to improve consistency and comparability across studies measuring human dietary exposure.
Identifying Microplastic Contamination in Marine Biota
This study developed and evaluated methods for identifying microplastic contamination in marine biota, comparing visual sorting, spectroscopic, and chemical digestion approaches for extracting and confirming plastic particles from tissue samples.
Rapid optimization of decapod crustacean digestion for efficient microplastic extraction (MPs)
Researchers developed and compared four digestion protocols for extracting microplastics from commercial crab samples, evaluating potassium hydroxide thermo-alkaline digestion, hydrogen peroxide oxidative digestion, protease enzymatic digestion, and dodecyl dimethyl betaine surfactant digestion. Protocols 2 and 4 achieved digestion efficiencies of 93% and 96% respectively, with good recovery rates and preservation of microplastic chemical integrity confirmed via FTIR.
Validation of an optimised protocol for quantification of microplastics in heterogenous samples: A case study using green turtle chyme
This paper developed and validated an optimized protocol for extracting and quantifying microplastics from the gut contents of marine organisms. Having a reliable, standardized method is essential for generating consistent data on microplastic ingestion across different species and studies.
Methodology for microplastics quantification in clams
Researchers developed a simplified methodology for extracting and quantifying microplastics from clam tissue, addressing the need for standardized protocols to assess microplastic accumulation in seafood that poses direct human exposure risk when consumed whole.
Efficiency assessment of microplastic extraction from green mussel \(\textit{Perna viridis}\) Linnaeus
This study evaluated and optimized laboratory extraction methods for isolating and identifying microplastics from the tissues of green mussels, which accumulate plastic particles through their filter-feeding habits. Reliable extraction methods are essential for accurately quantifying microplastic contamination in shellfish consumed by humans.
Optimization of an Analytical Protocol for the Extraction of Microplastics from Seafood Samples with Different Levels of Fat
Researchers optimized an analytical protocol for extracting microplastics from seafood samples with varying fat content, addressing a key methodological challenge in accurately quantifying microplastic contamination in marine food sources given the ubiquitous presence of plastic particles smaller than 5 mm in marine environments.
Occurrence, Distribution, and Extraction Methods of Microplastics in Marine Organisms
This review synthesizes global data on microplastic occurrence and distribution across marine organisms, comparing the advantages and limitations of different extraction and identification methods used in the field.
Optimization of a protocol for the extraction and chemical characterization of microplastics in Chamelea gallina by Raman microspectroscopy
Researchers optimized an extraction and chemical characterization protocol for microplastics in bivalve molluscs, improving polymer identification through combined spectroscopic methods. The validated protocol provides a reliable approach for routine microplastic monitoring in shellfish used as bioindicators.
Plastic contamination in commercially valuable decapods caught near a major Australian urban centre
Researchers examined the digestive tracts of four commercially harvested decapod crustacean species near Perth, Western Australia for microplastics, and tested muscle and hepatopancreas tissue for plasticizer chemicals. Microplastics were found in all species' guts, and phthalate and bisphenol plasticizers were detected in edible tissues, raising food safety concerns for local seafood consumers.
Extraction method development for nanoplastics from oyster and fish tissues
Researchers developed a method for extracting nanoplastics from oyster and fish tissues using enzymatic digestion, sequential membrane filtration, and purification steps, addressing a critical methodological gap for assessing nanoplastic contamination in seafood.
Microplastic in marine environment: reworking and optimisation of two analytical protocols for the extraction of microplastics from sediments and oysters
Researchers improved existing protocols for extracting microplastics from both marine sediments and oysters, making the NOAA protocol more versatile and extending it to PET extraction. Validated extraction methods are essential for producing consistent, comparable data on microplastic contamination in seafood.
Microplastics in decapod crustaceans: Accumulation, toxicity and impacts, a review
This review summarizes research on microplastic contamination in decapod crustaceans like shrimp, crabs, and lobsters, which are widely consumed as seafood. Studies have found microplastics accumulating in their gills, digestive organs, and gut, with experimental evidence showing oxidative stress, immune damage, and reproductive toxicity. The findings raise concerns about potential human exposure to microplastics through seafood consumption.
Microplastics ingestion by deep-sea decapod crustaceans from the Western Mediterranean
Researchers investigated microplastic ingestion by four deep-sea decapod crustacean species in the Sardinian and Catalan regions of the Western Mediterranean, analyzing gastrointestinal tracts from 210 individuals to assess abundance, characterization, and species- and region-level differences, finding ingestion prevalence of approximately 70%.
Microplastic Extraction from Marine Vertebrate Digestive Tracts, Regurgitates and Scats: A Protocol for Researchers from All Experience Levels
This paper presents a standardized protocol for extracting and identifying microplastics from the digestive systems of marine vertebrates (mammals, birds, turtles, and fish). Having consistent, accessible methods is essential for generating comparable data on how much plastic wildlife across different species and regions are ingesting.
Microplastics extraction from oyster tissue v1
Researchers developed and validated a protocol for extracting microplastics from oyster tissue, addressing the challenges of isolating plastic particles from a complex biological matrix that includes lipids, proteins, and mineral content. The method enables reliable quantification of microplastic contamination in bivalves — a widely consumed seafood and established bioindicator of coastal pollution — supporting standardised monitoring of microplastic uptake in marine food species.
Optimization of a new multi-reagent procedure for quantitative mussel digestion in microplastic analysis
This study optimized a chemical digestion protocol for extracting microplastics from mussel tissue by testing combinations of multiple reagents, aiming to completely destroy biological material while preserving plastic particles for analysis. The optimized method improved microplastic recovery compared to single-reagent approaches. Accurate extraction methods are critical for reliably measuring microplastic contamination in mussels, a widely consumed shellfish and important food safety indicator.
Microplastic contamination in brown shrimp (Crangon crangon, Linnaeus 1758) from coastal waters of the Southern North Sea and Channel area
Researchers examined brown shrimp caught from the North Sea and found microplastics in their digestive tracts, documenting contamination in a commercially important crustacean consumed widely by humans in northern Europe.