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20 resultsShowing papers similar to Advancements in optical techniques for direct identification and localization of micro- and nanoplastics in biological samples
ClearAdvancements in optical techniques for direct identification and localization of micro- and nanoplastics in biological samples
This review surveyed advances in optical techniques for detecting and localizing microplastics directly in biological tissue samples, addressing the challenge that human tissue detection has not kept pace with detection in environmental matrices. Emerging methods including Raman microspectroscopy and CARS microscopy were identified as most promising for tissue-level microplastic identification.
Localisation and identification of polystyrene particles in tissue sections using Raman spectroscopic imaging
Researchers developed a Raman spectroscopic imaging method to localize and identify polystyrene microplastic particles directly within tissue sections, enabling in-situ detection without fluorescent labeling and making environmental sample analysis feasible.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence microscopy, second harmonic generation imaging, and coherent Raman scattering to detect and map micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics were found to cross the blood-brain barrier and accumulate in lipid-rich brain regions in animal models.
Advanced Optical Imaging Technologies for Microplastics Identification: Progress and Challenges
This review surveys advanced optical imaging technologies used to identify microplastics and nanoplastics in environmental samples. Researchers compared techniques like Raman spectroscopy, infrared imaging, and fluorescence microscopy, noting their strengths and limitations for detecting increasingly small particles. The study highlights that improving detection methods is essential for accurately monitoring the full scope of plastic pollution.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence, second harmonic generation, and coherent Raman scattering microscopy in a single instrument to image micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics crossed the blood-brain barrier and accumulated in lipid-rich brain regions in mouse models.
Optical photothermal infrared spectroscopic assessment of microplastics in tissue models and non-digested human tissue sections
Researchers developed a method using optical photothermal infrared spectroscopy to detect and map microplastics directly within tissue sections without requiring chemical or enzymatic digestion. The study suggests this approach preserves spatial information about where microplastics are located within tissue architecture, overcoming a key limitation of conventional digestion-based methods that can lose some particles.
Identification and visualisation of microplastics/nanoplastics by Raman imaging (i): Down to 100 nm
Researchers developed an advanced Raman imaging technique capable of identifying and visualizing nanoplastics down to 100 nanometers in size. The study addressed a key analytical gap, as nanoplastic research has been limited by the lack of effective characterization methods, and the new approach offers a way to detect these extremely small particles that may pose greater environmental risks due to their high surface area.
How to Identify and Quantify Microplastics and Nanoplastics Using Raman Imaging?
This paper reviews advances in Raman imaging as a method for identifying and quantifying microplastics and nanoplastics in environmental samples, discussing current protocols, analytical challenges, and the need for standardization.
Direct identification and visualisation of real-world contaminating microplastics using Raman spectral mapping with multivariate curve resolution-alternating least squares
Multivariate curve resolution applied to Raman spectral maps allowed direct identification and visualization of microplastics in complex environmental and tissue matrices without prior physical separation, demonstrating that this chemometric approach reduces analysis time while improving sensitivity for detecting sub-20-micrometer particles.
Fast detection and 3D imaging of nanoplastics and microplastics by stimulated Raman scattering microscopy
Researchers developed a fast imaging technique using stimulated Raman scattering microscopy to detect and create 3D maps of nanoplastics and microplastics at the single-particle level. The method can identify plastic particles as small as 100 nanometers and distinguish between different polymer types without the need for dyes or labels. This technology could help scientists more accurately track tiny plastic particles in environmental and biological samples.
Raman spectroscopy: Recent advances in fast and reliable microplastic analysis
This review summarized recent advances in Raman spectroscopy for fast and reliable microplastic identification, covering improvements in speed, sensitivity, and automation that are making the technique more practical for routine environmental monitoring. Raman-based methods are increasingly able to identify microplastics in complex environmental matrices including biological tissues.
Label-free detection of polystyrene nanoparticles in Daphnia magna using Raman confocal mapping
Researchers demonstrated that Raman confocal mapping can detect polystyrene nanoparticles inside Daphnia magna without labels or dyes, revealing particle accumulation in the gut and providing a non-invasive method for studying nanoplastic uptake in organisms.
Raman Imaging Spectroscopy: History, Fundamentals and Current Scenario of the Technique
This review covers the history and principles of Raman imaging spectroscopy, a technique increasingly used to identify and map the chemical composition of microplastics in environmental samples. The review provides technical context for one of the most important tools in microplastic analysis.
Raman microspectroscopy and laser-induced breakdown spectroscopy for the analysis of polyethylene microplastics in human soft tissues
Researchers developed a combined technique using Raman microspectroscopy and laser-based analysis to detect polyethylene microplastics in human soft tissue samples. The method can identify both the plastic polymer and any associated inorganic elements in tissue. This kind of detection tool is important for understanding whether microplastics accumulate in specific human organs and what health effects they might have.
Label-free stimulated Raman scattering imaging of intracellular microplastics in mammalian cells
Researchers used label-free stimulated Raman scattering imaging to visualize microplastic uptake and distribution inside mammalian cells without fluorescent labels, finding that intracellular microplastics were associated with elevated reactive oxygen species, reduced cell viability, and altered lipid metabolism.
Identification and visualisation of microplastics/ nanoplastics by Raman imaging (ii): Smaller than the diffraction limit of laser?
Researchers examined whether confocal Raman microscopy can identify and visualize nanoplastics smaller than the diffraction limit of the laser, analyzing the lateral intensity distribution of Raman signals from nanoplastics ranging from approximately 30 to 600 nm in diameter. The study found that while imaging resolution is limited by diffraction, chemical identification of sub-diffraction-limit nanoplastics remains possible.
Misinterpretation in microplastic detection in biological tissues: When 2D imaging is not enough
Researchers demonstrated that 2D Raman imaging alone can misidentify microplastics in biological tissues, showing that 3D confocal Raman imaging is necessary to accurately distinguish microplastic particles from tissue components in mussels.
Label-free nano- and microplastics detection in mammalian tissue by photothermal infrared spectroscopy
Researchers applied optical photothermal infrared spectroscopy to detect and identify nano- and microplastics smaller than 1 µm in mammalian tissue sections without requiring labels or lengthy digestion protocols. The method successfully localized polystyrene particles in tissue samples with chemical specificity, offering a faster workflow for nanoplastic detection in biological matrices.
Raman spectroscopy: Recent advances in fast and reliable microplastic analysis
This review covered recent advances in Raman spectroscopy for fast and reliable microplastic analysis, with emphasis on its ability to characterize small particles that can penetrate living tissues. The authors highlight improvements in speed and sensitivity that are making Raman techniques more practical for environmental monitoring.
Identification and visualisation of microplastics by Raman mapping
Researchers demonstrated that Raman mapping can identify and visualize microplastics within soil and sand samples with minimal sample preparation. The technique successfully detected various polymer types against complex natural backgrounds without requiring dyes or destructive processing. The study presents Raman mapping as a practical, non-destructive analytical tool for studying microplastic distribution in environmental matrices like soil.