Papers

61,005 results
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Article Tier 2

An Overview into Polyethylene Terephthalate (PET) Hydrolases and Efforts in Tailoring Enzymes for Improved Plastic Degradation

This review examines the discovery and engineering of PET-degrading enzymes including PETase and cutinase variants, discussing protein engineering strategies to improve catalytic efficiency and thermostability for practical biodegradation of polyethylene terephthalate plastic waste.

2022 International Journal of Molecular Sciences 120 citations
Article Tier 2

Current knowledge on enzymatic PET degradation and its possible application to waste stream management and other fields

This review distinguished between enzymatic PET surface modification (useful for fiber treatment) and enzymatic PET degradation (needed for waste management), cataloguing the hydrolases capable of each function and the conditions required. The authors evaluate the prospects for deploying PET-degrading enzymes in industrial plastic waste streams.

2019 Applied Microbiology and Biotechnology 576 citations
Article Tier 2

Enzymatic Remediation of Polyethylene Terephthalate (PET)–Based Polymers for Effective Management of Plastic Wastes: An Overview

Enzymatic approaches for remediating PET-based plastic waste were reviewed, covering PETase and related enzymes that can break PET into reusable monomers. Enzyme engineering strategies to improve thermostability and catalytic efficiency are discussed as a pathway to scalable biological PET recycling.

2020 Frontiers in Bioengineering and Biotechnology 193 citations
Article Tier 2

Recent advances in enzyme engineering for improved deconstruction of poly(ethylene terephthalate) (PET) plastics

This review covers recent progress in engineering enzymes that can break down PET plastic, the material used in water bottles and food containers. While natural enzymes that digest PET have been discovered, they are not yet fast or durable enough for industrial-scale recycling. Advances in protein engineering, directed evolution, and computational design are steadily improving these enzymes, which could eventually provide a sustainable way to recycle PET and reduce microplastic pollution at its source.

2025 Communications Materials 7 citations
Article Tier 2

Enzymatic Degradation of PET plastic

This study tested commercial-grade enzymes for degrading PET plastic and found that enzymatic degradation was effective at laboratory scale but faced challenges for real-world application. Scaling up enzymatic PET recycling could reduce the persistence of plastic waste that eventually fragments into microplastics in the environment.

2023 ARPHA Conference Abstracts
Article Tier 2

Marine PET Hydrolase (PET2): Assessment of Terephthalate- and Indole-Based Polyester Depolymerization

This study characterized a marine-derived enzyme (PET2) capable of breaking down PET plastic under mild conditions, assessing its efficiency for enzymatic recycling. Enzyme-based PET recycling could prevent plastic waste from fragmenting into the microplastics that accumulate in oceans and organisms.

2023 Catalysts 2 citations
Article Tier 2

Biểu hiện, tinh sạch và đánh giá sơ bộ hoạt tính phân hủy nhựa PET của enzyme PETase tái tổ hợp

Vietnamese researchers successfully expressed and purified recombinant PETase enzyme — which breaks down PET plastic — finding optimal expression conditions and that adding glycerol and DTT enhanced its plastic-degrading activity. This is directly relevant to microplastic research as PETase-based biodegradation is a promising biological approach to reducing PET plastic waste and microplastic generation.

2023 Journal of Science and Technology
Article Tier 2

Process development for PETase production and purification

Researchers developed a production and purification process for PETase, an enzyme capable of breaking down polyethylene terephthalate (PET) plastic biologically, as an alternative to inadequate mechanical and chemical recycling methods for mixed and contaminated PET waste. The study addresses the global plastic pollution crisis by advancing the scalability of enzymatic PET degradation as a sustainable recycling pathway.

2024
Article Tier 2

Marine PET Hydrolase (PET2): Assessment of Terephthalate- and Indole-Based Polyesters Depolymerization

Researchers characterized a marine enzyme (PET2) capable of breaking down PET plastic and related polyester materials under relatively mild conditions. Discovering and engineering enzymes that can degrade PET could help address the massive accumulation of PET microplastics in ocean environments.

2023 Preprints.org 1 citations
Article Tier 2

Silica immobilized PETase for microplastic bioremediation: Influence of linker peptides on activity

Researchers immobilized a modified PETase enzyme onto silica using different linker peptides and tested its ability to break down PET microplastics, finding that linker peptide design significantly influenced enzyme activity and reusability — key parameters for practical application in wastewater treatment.

2025 Enzyme and Microbial Technology
Article Tier 2

Determinants for an Efficient Enzymatic Catalysis in Poly(Ethylene Terephthalate) Degradation

This review covers the current state of enzymatic PET degradation, examining which enzymes act on PET, how protein engineering has improved their activity, and what challenges remain before enzymatic recycling can be deployed at industrial scale.

2023 Catalysts 19 citations
Article Tier 2

Application of PETase in Plastic Biodegradation and Its Synthesis

This review examines how PETase enzymes can be used to biodegrade plastic waste, particularly polyethylene terephthalate, which is one of the most widely used plastics globally. Researchers discuss recent advances in modifying PETase enzymes for improved efficiency and establishing sustainable synthesis platforms. The study suggests that enzymatic biodegradation offers a promising biological solution to the growing plastic pollution crisis.

2024 E3S Web of Conferences 2 citations
Article Tier 2

Enhancing PET Degrading Enzymes: A Combinatory Approach

Scientists worked on improving enzymes that can break down PET plastic, one of the most common plastics in consumer products. Using a combinatory approach, researchers enhanced the performance of a naturally occurring PET-degrading enzyme from the bacterium Piscinibacter sakaiensis. The study suggests that engineered enzymes could eventually help create a circular economy for plastic waste by enabling efficient recycling at the molecular level.

2024 ChemBioChem 21 citations
Article Tier 2

Development of Enzyme-Based Approaches for Recycling PET on an Industrial Scale

This paper reviews the development of enzyme-based methods for breaking down PET plastic (used in bottles and packaging) at an industrial scale. While enzymatic recycling is a promising solution to plastic waste, current methods are still too slow and costly for widespread use. Improving these technologies could help reduce the enormous amount of PET entering the environment and breaking down into microplastics.

2024 Biochemistry 22 citations
Article Tier 2

Eco-Microbiology: Discovering Biochemical Enhancers of PET Biodegradation by Piscinibacter sakaiensis

This paper reviews biochemical strategies for enhancing PET biodegradation by microorganisms, focusing on the discovery and engineering of plastic-degrading enzymes. The review highlights recent advances and remaining challenges in scaling up enzymatic plastic degradation for industrial applications.

2024
Article Tier 2

The Current State of Research on PET Hydrolyzing Enzymes Available for Biorecycling

This review summarizes the current state of PET-hydrolyzing enzymes, including thermophilic cutinases and engineered variants, that are candidates for enzymatic biorecycling of PET plastic waste back into reusable monomers.

2021 Catalysts 91 citations
Article Tier 2

Engineered polyethylene terephthalate hydrolases: perspectives and limits

This review examines progress in engineering enzymes that can break down PET plastic, the material used in most beverage bottles and synthetic textiles. Researchers found that while significant advances have been made through protein engineering and machine learning, no enzyme yet exists that can efficiently degrade the crystalline form of PET found in real-world waste. The study outlines the key challenges remaining before enzymatic plastic recycling can work at industrial scale, including handling microplastic contamination.

2024 Applied Microbiology and Biotechnology 27 citations
Article Tier 2

Evaluation of enzymatic degradation of petase against PBT and PEN nanoplastics using dye release assay

Researchers expressed, purified, and tested wild-type and engineered variants of PETase enzyme against polyethylene naphthalate and polybutylene terephthalate nanoplastic substrates, developing a dye-release fluorescence assay that correlated with HPLC measurements to enable rapid, quantitative screening of enzymatic plastic degradation.

2025 DR-NTU (Nanyang Technological University)
Article Tier 2

Recent advances in nanotechnology-based modifications of micro/nano PET plastics for green energy applications.

This review covers nanotechnology-based approaches for recycling and degrading PET plastic, including use of catalysts and enzymatic methods. Since PET is one of the most widely produced and discarded plastics, developing better recycling methods is important for reducing the amount that fragments into environmental microplastics.

2024 Chemosphere
Article Tier 2

Characterization and engineering of a plastic-degrading aromatic polyesterase

Researchers characterized and engineered an aromatic polyesterase enzyme capable of degrading plastic polymers, improving its activity through protein engineering and demonstrating its potential as a tool for biodegradation-based plastic cleanup.

2018 Proceedings of the National Academy of Sciences 1006 citations
Article Tier 2

Targeted aggregation of PETase towards surface of Stenotrophomonas pavanii for degradation of PET microplastics

Researchers developed a strategy to target PETase enzyme to the surface of Stenotrophomonas pavanii bacteria, improving the efficiency of in-situ PET microplastic degradation. Surface-displayed PETase showed significantly enhanced PET hydrolysis compared to free enzyme, offering a practical approach to microbial degradation of dispersed PET microplastics in environmental settings.

2024 Journal of Hazardous Materials 9 citations
Article Tier 2

Recent advances in screening and identification of PET-degrading enzymes

Researchers reviewed recent advances in discovering and engineering enzymes capable of breaking down PET plastic, one of the most widely produced and persistent plastic types. They examined screening methods including metagenomic mining and machine learning approaches that have accelerated the identification of promising PET-degrading enzymes. The study suggests that enzymatic recycling could become a viable, environmentally friendly alternative to traditional PET disposal methods.

2024 Environmental Reviews 7 citations
Article Tier 2

Advancing PET-Degrading Enzymes through Directed Evolution to Combat Plastic Pollution

This review examines advances in directed evolution of PET-degrading enzymes including PETases and cutinases, describing how techniques such as error-prone PCR, DNA shuffling, and saturation mutagenesis have produced enzyme variants with improved catalytic efficiency and thermostability for enzymatic plastic recycling applications.

2025 American journal of student research.
Article Tier 2

Enzymatic PET Degradation

This review examines enzymatic degradation of PET (polyethylene terephthalate), the plastic used in bottles and polyester clothing, as a promising pathway for breaking down this persistent polymer. Advances in engineering more efficient PET-degrading enzymes could enable industrial-scale biological recycling and reduce the environmental accumulation of PET microplastics.

2019 CHIMIA International Journal for Chemistry 50 citations