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Labeling of PET and PP nanoplastic test materials with non-leachable π-conjugated fluorescent polymers
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Researchers produced fluorescently labeled PET and PP nanoplastic particles using co-precipitation with a conjugated polymer dye, achieving over 85% dye internalization and submicron particle sizes, and demonstrated their use for measuring cell uptake while overcoming dosimetry challenges posed by buoyant particles.
Abstract Micro- and nanoplastic (MNP) particles are widely present in nature, mainly due to the extensive overuse of single-use plastics combined with poor waste management. Despite the diversity in the environment, many experimental studies still rely almost exclusively on polystyrene as a model plastic test material, while other environmentally relevant polymers remain underrepresented. In addition, labeled MNP test materials suitable for biological studies are still limited. In this study, nanosized polyethylene terephthalate (PET) and polypropylene (PP) particles were produced using a co-precipitation approach with the fluorescent π-conjugated polymer, poly(9,9-dioctylfluorene-alt-benzothiadiazole) (F8BT) at low (0.8 % w/w) and high (3–5 % w/w) F8BT content. Fluorescently labeled MNPs (75–85 % dye internalization) could be produced with > 85 % of the particles in the submicron size range. Co-precipitation of F8BT with PET produced a subset of spindle-shaped particles, while F8BT-labeled PP particles were primarily spherical. The fluorescence limit of detection of the F8BT labeled PET and PP was ~0.2 µg/mL for both systems. The strong fluorescence enabled measurements of cell uptake using an innovative exposure system, the FlowCube, which overcomes dosimetry issues with buoyant particles. This work provides an innovative approach to producing fluorescently labeled PET and PP nanoplastic test materials for environmental and biological studies.
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This study developed fluorescently labeled plastic nanoparticles made from PET, polypropylene, and polystyrene that can be tracked in biological environments to study how nanoplastics are taken up and processed by living organisms. Having trackable model nanoplastics is an important tool for understanding how these particles move through tissues and food chains.
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Fluorescent polyethylene terephthalate (PET) nanoparticles with a hydrodynamic diameter of 158 nm were synthesized in a bottom-up approach for use as research tools. Concentration-dependent uptake and cytotoxicity were demonstrated in macrophages, providing well-characterized PET nanoplastic models for studying cellular interactions.
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