In vitro effects of polyethylene microplastics on primary cultures and cell lines of fish

Diego Romero García; Cristóbal Espinosa‐Ruíz; María Ángeles Esteban Abad

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In vitro effects of polyethylene microplastics on primary cultures and cell lines of fish

Dialnet (Universidad de la Rioja) 2018

Diego Romero García, Cristóbal Espinosa‐Ruíz, María Ángeles Esteban Abad

Summary

This study developed and validated an enzymatic purification protocol (BEPP) for isolating microplastics from biological matrices prior to µFTIR analysis. The protocol effectively digested organic tissue while preserving particle integrity, enabling accurate quantification and polymer identification in fish and invertebrate samples.