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Disruption of poly (3-hydroxyalkanoate) depolymerase gene and overexpression of three poly (3-hydroxybutyrate) biosynthetic genes improve poly (3-hydroxybutyrate) production from nitrogen rich medium by Rhodobacter sphaeroides
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Bacterial genes were engineered to improve production of PHB, a natural biodegradable plastic substitute, in bacteria that grow without using sugar as a carbon source. More efficient bio-based plastic production could help reduce dependence on petroleum-derived plastics.
In this study, we identified eight different genes involved in PHB biosynthesis in the genome of R. sphaeroides 2.4.1, and revealed that their overexpression increased PHB accumulation in an R. sphaeroides HJ strain. In addition, we demonstrated the effectiveness of a phaZ disruption for high PHB accumulation, especially under nitrogen rich conditions. Furthermore, we showed that PNS bacteria may have some unidentified genes involved in poly (3-hydroxyalkanoates) (PHA) biosynthesis. Our findings could lead to further improvement of environmentally harmless PHA production techniques using PNS bacteria.
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Poly(3-hydroxybutyrate) production by Rhodopseudomonas sp. S16-VOGS3 cells grown in digested sludge
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Researchers demonstrated that photosynthetic bacteria can be grown using nutrient-rich wastewater sludge as a low-cost medium, producing a biodegradable plastic called PHB (polyhydroxybutyrate) when phosphate becomes scarce. This approach offers a way to create eco-friendly bioplastics from waste materials, reducing reliance on petroleum-based plastics.
The Halophilic Bacterium Paracoccus haeundaensis for the Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) from Single Carbon Sources
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This study demonstrated for the first time that Paracoccus haeundaensis can produce polyhydroxyalkanoates (PHAs) under nitrogen-limited conditions with glucose as carbon source, identifying it as a potential new bacterial host for biodegradable plastic production.
Screening, identification and characterisation of Polyhydroxybutyrate producing bacteria from garden soil
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Researchers screened soil bacteria for their ability to produce polyhydroxybutyrate (PHB), a biodegradable biopolymer that can replace petroleum-based plastics. Five promising bacterial isolates were identified and characterized, with production conditions optimized. Scaling up microbial PHB production from inexpensive soil microbes is an important step toward making biodegradable plastic alternatives cost-competitive with conventional plastics.
Characterization of Polyhydroxybutyrate Producing Bacterium Isolated from Sewage Sample
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Researchers identified and characterized bacterial strains isolated from sewage samples that are capable of producing polyhydroxybutyrate (PHB), a biodegradable thermoplastic. The study screens natural microbial communities for strains useful in sustainable bio-based plastic production.
A Review of PHB Production by Cyanobacteria and Its Applications
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This review examines cyanobacteria as photoautotrophic producers of polyhydroxybutyrate (PHB) bioplastic, summarizing how nutrient-stress conditions stimulate PHB accumulation and evaluating the cost-effectiveness and sustainability of using cyanobacteria as an alternative to conventional feedstocks for biodegradable plastic production.
Research digests by email
When a large batch of papers lands in the Atlas, we read through it and send a short write-up of what stood out.