We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Enhanced production of biobased, biodegradable, Poly(3-hydroxybutyrate) using an unexplored marine bacterium Pseudohalocynthiibacter aestuariivivens, isolated from highly polluted coastal environment
Summary
Researchers isolated and characterised Pseudohalocynthiibacter aestuariivivens P96, a marine bacterium from a highly polluted coastal environment, finding it capable of producing poly(3-hydroxybutyrate) (PHB) at up to 4.73 g/L corresponding to 87% of total cell dry weight, representing a promising bio-based and biodegradable alternative to fossil-fuel plastics.
The production and disposal of plastics from limited fossil reserves, has prompted research for greener and sustainable alternatives. Polyhydroxyalkanoates (PHAs) are biocompatible, biodegradable, and thermoprocessable polyester produced by microbes. PHAs found several applications but their use is limited due to high production cost and low yields. Herein, for the first time, the isolation and characterization of Pseudohalocynthiibacter aestuariivivens P96, a marine bacterium able to produce surprising amount of PHAs is reported. In the best growth condition P96 was able to reach a maximum production of 4.73 g/L, corresponding to the 87 % of total cell dry-weight. Using scanning and transmission microscopy, lab-scale fermentation, spectroscopic techniques, and genome analysis, the production of thermoprocessable polymer Polyhydroxybutyrate P(3HB), a PHAs class, endowed with mechanical and thermal properties comparable to that of petroleum-based plastics was confirmed. This study represents a milestone toward the use of this unexplored marine bacterium for P(3HB) production.
Sign in to start a discussion.
More Papers Like This
The Halophilic Bacterium Paracoccus haeundaensis for the Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) from Single Carbon Sources
This study demonstrated for the first time that Paracoccus haeundaensis can produce polyhydroxyalkanoates (PHAs) under nitrogen-limited conditions with glucose as carbon source, identifying it as a potential new bacterial host for biodegradable plastic production.
Optimized Poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHBV) Production by Moderately Haloalkaliphilic Bacterium Halomonas alkalicola Ext
Researchers isolated a salt- and alkali-tolerant bacterium from a Kenyan lake and optimized its production of the biodegradable polymer PHBV as an alternative to conventional plastics. Through systematic optimization of growth conditions, they achieved a polymer yield of over 45% of the bacterial cell mass. The study demonstrates that extremophilic microorganisms can serve as efficient producers of biodegradable plastics suitable for packaging and biomedical applications.
Poly(3-hydroxybutyrate) production by Rhodopseudomonas sp. S16-VOGS3 cells grown in digested sludge
Researchers demonstrated that photosynthetic bacteria can be grown using nutrient-rich wastewater sludge as a low-cost medium, producing a biodegradable plastic called PHB (polyhydroxybutyrate) when phosphate becomes scarce. This approach offers a way to create eco-friendly bioplastics from waste materials, reducing reliance on petroleum-based plastics.
Selection of polyhydroxybutyrate-producing bacteria and their polyhydroxybutyrate production using cassava and glycerol as carbon sources
Researchers selected effective polyhydroxybutyrate (PHB)-producing bacteria and evaluated their ability to produce PHB using low-cost carbon sources including cassava and glycerol, aiming to reduce the high production costs that limit PHB bioplastics as a competitive alternative to synthetic plastics.
Disruption of poly (3-hydroxyalkanoate) depolymerase gene and overexpression of three poly (3-hydroxybutyrate) biosynthetic genes improve poly (3-hydroxybutyrate) production from nitrogen rich medium by Rhodobacter sphaeroides
Bacterial genes were engineered to improve production of PHB, a natural biodegradable plastic substitute, in bacteria that grow without using sugar as a carbon source. More efficient bio-based plastic production could help reduce dependence on petroleum-derived plastics.