We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Characterizing nanoplastics‐induced stress and its SERS fingerprint in an intestinal membrane model
AI summary Read the abstract
Researchers used SERS (surface-enhanced Raman scattering) to detect nanoplastic-induced stress in a Caco-2 intestinal epithelial membrane model, finding that amine-functionalized polystyrene nanoparticles disrupted barrier function and produced distinct spectral fingerprints in the extracellular medium, demonstrating a non-invasive method for monitoring nanoplastic cellular stress.
Abstract Epithelium membranes provide important barrier functions, and it is important to understand how nanoparticle (NP) exposure affects their barrier function. In this manuscript, we investigate NP‐induced stress in a Caco‐2 intestinal epithelial membrane model and its effect on the vibrational spectrum of the extracellular medium that can be sampled and investigated without perturbation of the cells. Monolayers of Caco‐2 cells were incubated with 50 nm diameter polystyrene (PS) NPs functionalized with amine or carboxylic acid groups and concentrations of 1 × 10 12 –1 × 10 14 PS NPs mL –1 for 6 and 18 hours. Reactive oxygen species (ROS) generation, cell viability, and intestinal membrane integrity measurements were performed to detect and quantify PS NP‐induced membrane damage under the acute exposure conditions. After identifying conditions that result in NP‐induced stress, Surface Enhanced Raman Spectroscopy (SERS) was applied to monitor the composition of the medium in direct contact with the intestinal cells and to detect potential PS NP‐induced changes in the cellular metabolism in real time and in a minimally invasive fashion. The analysis of the SERS spectra through artificial intelligence algorithms and chemometric tools revealed concentration‐, exposure time‐, and surface chemistry‐dependent differences in the cellular metabolism in response to PS NPs. The SERS spectral analysis identifies the ring breathing mode of hypoxanthine (C 4 H 4 N 4 O), as a spectroscopic marker for the PS NP‐induced loss in membrane integrity.
More Papers Like This
Probe Cytotoxic and Oxidative Stress Effects of Nanoplastics on Caco-2 Cells: Insights from Raman Spectroscopy and Machine Learning
AI summary Read the abstract
Human intestinal cells (Caco-2) exposed to polystyrene nano- and microparticles showed oxidative stress and cell death. Pretreating the cells with resveratrol lowered reactive oxygen species caused by amine-modified nanoplastics. The study also used Raman spectroscopy with machine learning to detect the damage.
Cell Responseto Nanoplastics and Their Carrier EffectsTracked Real-Timely with Machine Learning-Driven Smart Surface-EnhancedRaman Spectroscopy Slides
AI summary Read the abstract
Researchers developed smart SERS slides to monitor in real-time how cells respond to polystyrene nanoplastics and their ability to carry contaminants (the 'carrier effect'). The platform captured intracellular metabolic changes at the molecular level, showing nanoplastics extended cell cycle S and G2 phases in lung cells and that carried pollutants caused additional distinct cellular effects.
Identification of polystyrene nanoplastics using surface enhanced Raman spectroscopy
AI summary Read the abstract
Researchers demonstrated for the first time that surface-enhanced Raman spectroscopy (SERS) using silver nanoparticles can identify polystyrene nanoplastics as small as 50 nm in real water samples, providing a rapid detection method that bypasses conventional sample preparation and could advance environmental monitoring of nanoplastics previously invisible to standard analytical techniques.
Machine Learning-Aided 3D Dynamic SERS Strategy for Physiological Mapping: Biotoxicity of Environmentally Dimensional Aged Nanoplastics and Corresponding Protein Corona Complexes
AI summary Read the abstract
Researchers used a new combination of 3D surface-enhanced Raman spectroscopy and machine learning to study the toxicity of nanoplastics on cells. They found that aged nanoplastics and those coated with proteins from the environment caused different types of cell damage depending on the plastic type. This approach could help scientists more rapidly assess the biological hazards of nanoplastics found in the environment.
Quantifying Cellular Nanoplastic Accumulation Using Raman Microscopy
AI summary Read the abstract
Researchers used a laser-based imaging technique to measure how much tiny plastic (nanoplastic) built up inside human intestinal cells grown in a lab dish. The method tracked plastic levels in individual cells at two doses over 24 hours, finding that lower doses sometimes led to more plastic buildup than higher doses, which connected to differences in cell gene activity and survival.
Research digests by email
When a large batch of papers lands in the Atlas, we read through it and send a short write-up of what stood out.