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Effects induced by polyethylene microplastics oral exposure on colon mucin release, inflammation, gut microflora composition and metabolism in mice

Ecotoxicology and Environmental Safety 2021 238 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 60 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.

Di Wu, Xiaona Yang, Di Wu, Hanqing Sun, Di Wu, Na Chen, Di Wu, Xiaona Yang, Di Wu, Na Chen, Di Wu, Xiaona Yang, Di Wu, Xiaona Yang, Di Wu, Di Wu, Yankai Xia Xiaona Yang, Di Wu, Di Wu, Di Wu, Di Wu, Di Wu, Di Wu, Di Wu, Di Wu, Di Wu, Di Wu, Di Wu, Di Wu, Di Wu, Yankai Xia Yankai Xia Xiaona Yang, Yankai Xia Di Wu, Yankai Xia Yankai Xia Yankai Xia

Summary

Researchers fed mice polyethylene microplastics for 30 days and found that even low doses reduced protective mucus in the colon, altered inflammation markers, and shifted the composition of gut bacteria. The microplastics increased the ratio of Bacteroides to Firmicutes bacteria and affected metabolic pathways in the gut microbiome. The study suggests that oral microplastic exposure may disrupt intestinal health by modifying the gut microbial community and its metabolism.

Polymers

Body Systems

Digestive Immune

Models

Mouse

Microplastics are plastic fragments widely distributed in the environment and accumulate in the organisms. However, the research on microplastics effects in mammals is limited. Polyethylene is the main kind of microplastics in the environment. We hypothesized that polyethylene exposure disrupts host intestine metabolism by modifying intestine microflora composition and then lipopolysaccharide (LPS) pathway. Female mice were orally exposed to 0, 0.002 and 0.2 μg/g/d polyethylene microplastics (PE MPs) for 30 days. Colon mucin density was quantized after AB-PAS staining. Mucin 2 (MUC2), inflammatory factors (IL-1β, IL-6, IL-8 and IL-10), short-chain fatty acid receptors (GPR41 and GPR43), LPS receptors (TLR4 and MyD88) and LPS pathway downstream genes (ERK1 and NF-κB) mRNA levels in colon were measured. Feces were collected on the 15th day of exposure for gut microflora analysis. Blood biochemical analysis was performed. Results showed that 0.2 μg/g/d PE MPs exposure significantly decreased colon mucin expression (p < 0.05), decreased IL-1β (p < 0.05) and increased IL-8 and IL-10 levels (p < 0.01 and p < 0.001 respectively). Microflora data showed that in 0.2 μg/g/d PE MPs group the number of Firmicutes decreased and the number of Bacteroides increased (both p < 0.01). Predicted KEGG metabolic pathways by piecrust method indicated that PE MPs enhanced amino acids metabolism in microflora. ERK1 and NF-κB mRNA were significantly lower in 0.2 μg/g/d PE MPs group (both p < 0.001). Blood total protein, albumin and globulin levels significantly increased after 0.2 μg/g/d PE MPs exposure (p < 0.01, p < 0.01 and p < 0.05 respectively). These results indicate that PE MPs exposure induced decreased mucin production, a slight immune response and increased the microflora amino acid metabolism in the mice colon by modifying colon microflora composition. SUMMARY: Polyethylene microplastics exposure decreased colon mucin release and increased amino acid metabolism by modifying colon microflora composition.

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