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Distinct accumulation of nanoplastics in human intestinal organoids
Summary
Researchers exposed human intestinal organoids to polystyrene nanoplastics and found that these tiny particles accumulated in distinct patterns within the gut tissue model. The study observed that nanoplastic uptake increased with concentration and caused measurable changes in the intestinal cells. These findings provide early evidence that nanoplastics can be absorbed by human intestinal tissue, raising questions about potential long-term health effects from dietary exposure.
Plastic particles, especially nanoplastics, represent an emerging concern of threat to human health, oral uptake is an important pathway for the plastic particles ingestion by human. While their fate and adverse effects in animal gastrointestinal tract are increasingly investigated, knowledge about their uptake and toxicity in human intestine is still limited. Here, by exposing human intestinal organoids to polystyrene nanoplastics (PS-NPs, ~50 nm in size) with concentrations of 10 and 100 μg/mL, we present evidence of their distinct accumulation in various type cells in intestinal organoids, then causing the cell apoptosis and inflammatory response. Our results further revealed that the effective inhibition of PS-NPs accumulation in secretive cells through co-exposure to a clathrin-mediated endocytosis inhibitor (chlorpromazine), and proved the essential role of active endocytosis in the PS-NPs uptaking into enterocyte cells. Our work not only elucidated the potential uptake and toxicity of PS-NPs in human intestinal cells and the underlying mechanism, but also provide a potential therapeutic approach to relieve the toxicity of PS-NPs to human through the endocytosis inhibition.
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