We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Transcriptome analysis reveals the high temperature-induced damage is a significant factor affecting the osmotic function of gill tissue in cold-water fish
Summary
Researchers used transcriptome analysis to investigate how high-temperature stress damages a biological system, finding that heat is a significant factor altering gene expression. This molecular biology study examines the cellular response to thermal stress.
Abstract Background Maintaining osmotic balance plays an important role in the normal operation and survival of cold-water fish. High water temperature has been proven to reduce the activity of NA + -K + -ATPase in the gill. However, the mechanism of megatemperature affect gill osmoregulation has not been fully elucidated. Results In this study, A.baerii , as a kind of iconic cold-water fish, was used to analyze histological alterations, plasma ion content, and transcriptional responses of gill of A.baerii , subjected to 20℃, 24℃and 28℃. We found that ROS levels in gill tissue increased and the gill damage deteriorated along with the temperature rising. Moreover, plasma Cl − levels at 28℃ were much lower than those in the other two groups, and there were no significant differences in Na + and K + ions among all trials. At the same time, the transcriptome data indicated that there were significant changes in injury-related pathways and genes at 24℃and 28℃. Meanwhile, GO enrichment analysis showed a large number of genes related to ion transport function changed with temperature increasing during cell processes. We discovered that as temperature increased, the expression of genes that regulate ion outflow ( OCLN ) and active ion absorption ( AE2, NHE3, CLC-2 ) increased, while NKA-a expression decreased. Furthermore, the changes in osmotic regulation genes and the degree of tissue damage at 28℃ were significantly higher than those in the other two groups, indicating that the degree of heat stress-induced injury affects the osmotic regulation function of gill tissue. Conclusions this study reveals for the first time that the tissue damage caused by high temperature affects the function and the response mechanism of the osmotic regulation in gill of cold-water fish. It provides reference data for the study on the physiological function of cold-water fish under global warming.
Sign in to start a discussion.
More Papers Like This
Transcriptome Analysis of Gills Provides Insights Into Translation Changes Under Hypoxic Stress and Reoxygenation in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)
This transcriptome study found that oxygen deprivation suppresses immune genes in the gills of golden pompano fish, potentially explaining why fish are more susceptible to infections under low-oxygen aquaculture conditions. The molecular findings support improved oxygen management practices in fish farming to protect both animal welfare and food quality.
Transcriptome Analysis Revealed the Early Heat Stress Response in the Brain of Chinese Tongue Sole (Cynoglossus semilaevis)
Researchers used transcriptome analysis to study how the brains of Chinese tongue sole fish respond to short-term heat stress. They identified distinct gene expression patterns in male and female fish, with key pathways related to cortisol production, immune signaling, and stress-response genes like heat shock proteins. The study provides new insights into the sex-specific brain mechanisms fish use to cope with temperature changes.
Transcriptome analysis provides insights into copper toxicology in piebald naked carp (Gymnocypris eckloni)
Researchers exposed piebald naked carp to copper — a common water pollutant — and found it significantly disrupted gene activity in the gills and liver, particularly genes involved in building proteins, suggesting that fish respond to metal-induced stress by broadly slowing down protein production as a protective mechanism.
Transcriptome alterations in zebrafish gill after exposure to different sizes of microplastics
Researchers found that microplastic exposure in zebrafish gills caused size-dependent transcriptomic changes, with smaller particles triggering more differentially expressed genes related to immune response, oxidative stress, and apoptosis pathways compared to larger particles.
Transcriptomic, histological and biochemical analyses of Macrobrachium nipponense response to acute heat stress
This study examined how acute heat stress affects the physiology of the oriental river prawn, finding significant changes in gene expression and biochemistry that provide insight into how this commercially important species responds to warming water temperatures.