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Microbiome, mixotrophic algae, zooplankton, and fish amino acid and phospholipid fatty acid content in terrestrial and plastic carbon treatments
Summary
This duplicate dataset entry contains nutritional composition measurements from an aquatic food web experiment examining how plastic-derived carbon compares to natural leaf litter as an energy source across trophic levels. The study provides direct evidence of how microplastic carbon is incorporated into the food web.
Data includes amino acid (µg AA mg DW-1) and phospholipid fatty acid content (µg FA mg DW-1) of the microbiome, mixotrophic algae, zooplankton, and fish from the four-trophic level experiment. The experiment included control (no addition), 13.5% 13C-labelled beech leaves (Fagus sylvatica), 97% 13C-labelled lignin-hemicellulose extracted from wheat (Triticum aestivum, ~80% lignin, 13% hemicellulose), and 99% 13C-labelled polystyrene (microplastic). Incubation time in humic lake water was 14 days in the control, leaf, and lignin experiment but 56 days for polystyrene, which after mixotrophic algae (Cryptomonas sp.) was introduced to the bottles. In the next step, herbivorous zooplankton (Daphnia magna) consumed microbes, mixotrophic algae, and particles for five days which after they were used as the diet to zebrafish (Danio rerio) during a five-day experiment.