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Virgin and Aged Microplastics Induce Type-specific Inflammatory Responses on Vascular Cells

Research Square (Research Square) 2023 1 citation ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 40 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Tommaso Lomonaco, Elisa Persiani, Denise Biagini, Ilaria Gisone, Elisa Ceccherini, Antonella Cecchettini, Andrea Corti, Silvia Ghimenti, Fabio Di Francesco, Valter Castelvetro, Federico Vozzi

Summary

Both virgin and UV-aged polystyrene and polyethylene microplastics triggered inflammatory responses in human coronary artery smooth muscle cells in vitro, with different polymer types and aging states producing distinct patterns of cellular damage. The results suggest that microplastics ingested or inhaled by people may contribute to vascular inflammation and worsen cardiovascular disease — a significant human health concern.

Abstract Microplastics (MPs) are recognized as a major environmental problem due to their ubiquitous presence in ecosystems and bioaccumulation in food chains. Not only humans are continuously exposed to these pollutants through ingestion and inhalation, but recent findings suggest they may trigger vascular inflammation and potentially worsen the clinical conditions of cardiovascular patients. Here we combine headspace analysis by needle trap microextraction-gas chromatography-mass spectrometry and biological assays to evaluate the effects of polystyrene, high- and low-density polyethylene MPs on phenotype, metabolic activity, and pro-inflammatory status of Human Coronary Artery Smooth Muscle Cells (HCASMCs). Virgin and artificially aged MPs (4 weeks at 40 °C and 750 W/m 2 simulated solar irradiation) were comparatively tested at 1 mg/mL to simulate a realistic exposure scenario. Our results clearly show the activation of oxidative stress and inflammatory processes when HCASMCs were cultured with aged polymers, with significant overexpression of IL-6 and TNF-α. In addition, volatile organic compounds (VOCs), including pentane, acrolein, propanal, and hexanal as the main components, were released into the headspace. Type-specific VOC response profiles were induced on vascular cells from different MPs.

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