Exposure to epoxy-modified nanoplastics in the range of μg/L causes dysregulated intestinal permeability, reproductive capacity, and mitochondrial homeostasis by affecting antioxidant system in Caenorhabditis elegans

Although surface chemically modified nanopolystyrene (PS) has been reported to have potential toxicity toward organisms, the impact of epoxy modification on the toxicity of PS remains largely unknown. In this study, we first investigated the prolonged exposure effects of epoxy-modified PS (PS-CHO) in the range of μg/L on Caenorhabditis elegans (C. elegans) including general toxicity, target organ toxicity, and organelle toxicity. Our data revealed that C. elegans exposed to PS-CHO led to the alterations in increased lethality (≥ 1000 μg/L), shortened body length (≥ 100 μg/L), and decreased locomotion capacity (≥ 1 μg/L). In addition, toxicity analysis on target organs and organelles indicated that exposure to PS-CHO enhanced intestinal permeability (≥ 100 μg/L) by inhibiting the transcriptional levels of acs-22 (encoding fatty acid transport protein) (≥ 100 μg/L) and hmp-2 (encoding α-catenin) (≥ 1000 μg/L), reduced reproductive capacity (≥ 10 μg/L), and dysregulated mitochondrial homeostasis (≥ 1 μg/L). Moreover, the activation of antioxidant enzyme system could help nematodes against the toxicity caused by PS-CHO exposure (≥ 10 μg/L). Furthermore, we also compared the toxicity of PS-CHO with other chemically modified derivatives of PS, and the toxicity order was PS-NH > PS-SOOOH > PS-CHO > PS-COOH > PS > PS-PEG. Our study highlights the potential environmental impact of PS and its derivatives on organisms and suggests that the toxicity of nanoplastics may be charge-dependent.