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Regulation of target genes transcription in copepods Acartia tonsa waterborne or foodborne exposed to naked or metal-doped nanoplastics of different sizes and polymer composition

Zenodo (CERN European Organization for Nuclear Research) 2024 Score: 45 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.

Nagore González-Soto, Guillaume Bucher, Gabriella F. Schirinzi, Nagore González-Soto, Nagore González-Soto, Gabriella F. Schirinzi, Gabriella F. Schirinzi, Nagore González-Soto, Gabriella F. Schirinzi, Gabriella F. Schirinzi, Tamer Hafez, Tamer Hafez, Tamer Hafez, Nagore González-Soto, Gabriella F. Schirinzi, Tamer Hafez, Gorka Yabar, Gorka Yabar, Denise M. Mitrano, Nagore González-Soto, Gorka Yabar, Gorka Yabar, Tamer Hafez, Denise M. Mitrano, Tamer Hafez, Eider Bilbao, Nagore González-Soto, Gabriella F. Schirinzi, Gabriella F. Schirinzi, Gabriella F. Schirinzi, Guillaume Bucher, Miren P. Cajaraville, Miren P. Cajaraville, Denise M. Mitrano, Nagore González-Soto, Gabriella F. Schirinzi, Nagore González-Soto, Tamer Hafez, Miren P. Cajaraville, Eider Bilbao, Eider Bilbao, Douglas Gilliland, Douglas Gilliland, Denise M. Mitrano, Denise M. Mitrano, Miren P. Cajaraville, Miren P. Cajaraville, Miren P. Cajaraville, Miren P. Cajaraville, Amaia Orbea, Gabriella F. Schirinzi, Gabriella F. Schirinzi, Marisa Sárria Pereira De Passos, Marisa Sárria Pereira De Passos, Marisa Sárria Pereira De Passos, Marisa Sárria Pereira De Passos, Guillaume Bucher, Douglas Gilliland, Douglas Gilliland, Gabriella F. Schirinzi, Denise M. Mitrano, Amaia Orbea, Amaia Orbea, Eider Bilbao, Douglas Gilliland, Gabriella F. Schirinzi, Amaia Orbea, Amaia Orbea, Douglas Gilliland, Gabriella F. Schirinzi, Douglas Gilliland, Miren P. Cajaraville, Gabriella F. Schirinzi, Denise M. Mitrano Eider Bilbao, Eider Bilbao, Amaia Orbea, Amaia Orbea, Amaia Orbea, Miren P. Cajaraville, Gabriella F. Schirinzi, Miren P. Cajaraville, Eider Bilbao, Douglas Gilliland, Douglas Gilliland, Douglas Gilliland, Guillaume Bucher, Guillaume Bucher, Douglas Gilliland, Amaia Orbea, Douglas Gilliland, Douglas Gilliland, Douglas Gilliland, Douglas Gilliland, Douglas Gilliland, Miguel-Ángel Serra Beltran, Douglas Gilliland, Miguel-Ángel Serra Beltran, Miguel-Ángel Serra Beltran, Gabriella F. Schirinzi, Douglas Gilliland, Miguel-Ángel Serra Beltran, Denise M. Mitrano Denise M. Mitrano, Denise M. Mitrano, Miren P. Cajaraville, Miren P. Cajaraville, Douglas Gilliland, Denise M. Mitrano Miren P. Cajaraville, Miren P. Cajaraville, Amaia Orbea, Amaia Orbea, Miren P. Cajaraville, Miren P. Cajaraville, Eider Bilbao, Miren P. Cajaraville, Amaia Orbea, Eider Bilbao, Douglas Gilliland, Douglas Gilliland, Denise M. Mitrano, Denise M. Mitrano Denise M. Mitrano, Denise M. Mitrano

Summary

Researchers exposed the marine copepod Acartia tonsa to nanoplastics via waterborne and foodborne routes and measured transcriptional responses in target genes. Exposure route influenced gene expression patterns, with foodborne exposure producing distinct molecular responses compared to direct waterborne contact.

Polymers

PE PP PS PVC

Models

Invertebrate other

Nanoplastic (NP) pollution in aquatic ecosystems is of global concern. Metal-doped NPs are applied in toxicological studies to assess NPs fate in the organisms using ICP-MS. This work aimed to investigate transcriptional effects of waterborne or foodborne exposure to different NPs on the marine copepod Acartia tonsa. Copepods were exposed for 24 h to 100, 200, 500 or 1000 nm polystyrene (PS) NPs, 144 nm Pd-doped PS NPs or to 200 nm Au-doped polyvinyl chloride (PVC), polypropylene (PP) or polyethylene (PE) NPs. Exposures did not cause mortality or changes in ingestion rates of copepods. Transcription levels of ferritin, catalase, heat shock protein(hsp) 90 and hsp 70, and cyp3026b were quantified by RT-qPCR. In addition, accumulation of metal-doped NPs was measured in microalgae and copepods and their feces using ICP-MS. Results demonstrated that although PS NPs of different sizes did not affect mRNA levels, Pd-PS NPs caused significant changes in transcription of genes involved in general and oxidative stress. ICP-MS confirmed the accumulation of Pd-PS NPs in copepods exposed both through water and algae. Au-PP NPs also induced significant changes in copepods mRNA levels. In addition, the accumulation of Au-PP NPs in copepods was found to be higher compared to those exposed to Au-PVC or Au-PE NPs. The Au mass in foodborne Au-PVC and Au-PE NPs exposed copepods was slightly higher in feces than in copepods, suggesting that these were excreting the NPs through feces when fed with microalgae. This study demonstrates that NPs accumulation/excretion, as well as the molecular mechanisms regulated in A.tonsa, depend on the plastic polymer type, the presence of metals and the exposure route (waterborne versus foodborne). *Funding: EC CAS NANOPLASTICS project (DG JRC), Spanish MICINN FIERA project (PID2021-128600OB-I00), Basque Government (grant to consolidated research group IT1743-22 and postdoctoral grant to NGS) and Euskampus Fundazioa (LTC AquEus). Also see: https://micro2024.sciencesconf.org/559709/document

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