Fate, uptake and impact of fit-for-purpose nanoplastics on the digestive environment: an in vitro-in vivo continuum study
Zenodo (CERN European Organization for Nuclear Research)2024
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Muriel Mercier-Bonin,
Muriel Mercier-Bonin,
Chloé Liebgott,
Muriel Mercier-Bonin,
Chloé Liebgott,
Chloé Liebgott,
Chloé Liebgott,
Chloé Liebgott,
Chloé Liebgott,
Chloé Liebgott,
Chloé Liebgott,
Stéphanie Reynaud,
Stéphanie Reynaud,
Muriel Mercier-Bonin,
Melanie Mobley,
Melanie Mobley,
Sophie Miguel,
Catherine Beaufrand,
Stéphanie Reynaud,
Sophie Miguel,
Melanie Mobley,
Muriel Mercier-Bonin,
Catherine Beaufrand,
Sophie Miguel,
Sophie Miguel,
Melanie Mobley,
Valérie Bézirard,
Sophie Miguel,
Sophie Miguel,
Sophie Miguel,
Valérie Bézirard,
Sophie Miguel,
Catherine Beaufrand,
Sophie Miguel,
Sophie Miguel,
Stéphanie Reynaud,
Sophie Miguel,
Sophie Miguel,
Sophie Miguel,
Sophie Miguel,
Sophie Miguel,
Sophie Miguel,
Javier Jimenez-Lamana,
Javier Jimenez-Lamana,
Stéphanie Reynaud,
Stéphanie Reynaud,
Valérie Bézirard,
Stéphanie Reynaud,
Valérie Bézirard,
Valérie Bézirard,
Javier Jimenez-Lamana,
Javier Jimenez-Lamana,
Valérie Bézirard,
Valérie Bézirard,
Stéphanie Reynaud,
Stéphanie Reynaud,
Stéphanie Reynaud,
Stéphanie Reynaud,
Valérie Bézirard,
Bruno Grassl,
Catherine Beaufrand,
Bruno Grassl,
Stéphanie Reynaud,
Catherine Beaufrand,
Catherine Beaufrand,
Stéphanie Reynaud,
Stéphanie Reynaud,
Hélène Eutamène
Bruno Grassl,
Bruno Grassl,
Bruno Grassl,
Bruno Grassl,
Stéphanie Reynaud,
Catherine Beaufrand,
Catherine Beaufrand,
Bruno Grassl,
Bruno Grassl,
Hélène Eutamène,
Stéphanie Reynaud,
Javier Jimenez-Lamana,
Stéphanie Reynaud,
Stéphanie Reynaud,
Javier Jimenez-Lamana,
Javier Jimenez-Lamana,
Stéphanie Reynaud,
Stéphanie Reynaud,
Stéphanie Reynaud,
Stéphanie Reynaud,
Javier Jimenez-Lamana,
Stéphanie Reynaud,
Stéphanie Reynaud,
Stéphanie Reynaud,
Léa Spitzer,
Stéphanie Reynaud,
Léa Spitzer,
Stéphanie Reynaud,
Marie Tremblay‐Franco,
Marie Tremblay‐Franco,
Valérie Bézirard,
Roselyne Gautier,
Roselyne Gautier,
Roselyne Gautier,
Valérie Bézirard,
Roselyne Gautier,
Stéphanie Reynaud,
Valérie Bézirard,
Stéphanie Reynaud,
Roselyne Gautier,
Valérie Bézirard,
Roselyne Gautier,
Roselyne Gautier,
Roselyne Gautier,
Roselyne Gautier,
Roselyne Gautier,
Cécile Canlet,
Hervé Robert,
Hervé Robert,
Cécile Canlet,
Cécile Canlet,
Stéphanie Reynaud,
Hervé Robert,
Hervé Robert,
Cécile Canlet,
Bruno Grassl,
Hervé Robert,
Stéphanie Reynaud,
Bruno Grassl,
Bruno Grassl,
Hervé Robert,
Bruno Grassl,
Stéphanie Reynaud,
Stéphanie Reynaud,
Stéphanie Reynaud,
Stéphanie Reynaud,
Muriel Mercier-Bonin,
Stéphanie Reynaud,
Stéphanie Reynaud,
Stéphanie Reynaud,
Hervé Robert,
Hervé Robert,
Hervé Robert,
Hervé Robert,
Hélène Eutamène
Hélène Eutamène
Hélène Eutamène,
Hélène Eutamène,
Cécile Canlet,
Muriel Mercier-Bonin,
Muriel Mercier-Bonin,
Cécile Canlet,
Cécile Canlet,
Muriel Mercier-Bonin,
Muriel Mercier-Bonin,
Stéphanie Reynaud,
Stéphanie Reynaud,
Stéphanie Reynaud,
Hélène Eutamène,
Hélène Eutamène,
Hélène Eutamène
Hélène Eutamène,
Hélène Eutamène
Hélène Eutamène
Summary
Researchers investigated the fate, uptake, and impact of fluorescent and gold-labeled polystyrene nanoplastics on the digestive environment, using fit-for-purpose labeled particles to track nanoplastic behavior in biological systems. The labeled nanoplastics enabled detailed mapping of how plastic nanoparticles are processed in the gut, providing mechanistic insight into absorption pathways.
The potential hazards of nanoplastics (NPLs) to human health are poorly known, and interdisciplinary studies are crucial to address such complex issue. The originality of our study lies in the use of different fit-for-purpose labelled (i.e. fluorescent or gold-labelled) polystyrene NPL models. We investigated the impact of NPL exposure on the digestive environment using in vitro and in vivo approaches. Using the standardized in vitro intestinal digestion model INFOGEST 2.0, we studied the interactions between fluorescent NPLs and digestive fluids, simulating the salivary, gastric and intestinal phases. For the salivary phase, adsorption of amylase onto the particles was shown. Moreover, an agglomeration of NPLs during the gastric phase was observed, accompanied by adsorption of pepsin and lipase onto the particle surface, thus potentially influencing their activity and subsequently host's digestion capacities. By using a co-culture of Caco-2/HT29-MTX intestinal cells, we are now evaluating how gastrointestinal digestion affects the fate, uptake and toxicity of NPLs. In parallel, toxicity of gold-labelled NPLs was evaluated on a preclinical in vivo model with an intact intestinal barrier. Gold-core labelling enables to specifically trace NPLs within the whole organism using ICP-MS. Mice were exposed through drinking water to different doses of NPLs (0.1, 1 and 10 mg/kg body weight/day) for 90 days. Interestingly, despite NPLs weren't detected in intestinal tissues and peripherical organs, the main effects were observed at the lowest dose. In particular, NPL exposure modified intestinal gene expression of tight junctions (e.g. JamA, Zo1), pro-inflammatory cytokines (e.g. IL6, TNFa, IL1B) and anti-microbial peptides (e.g. Lysozyme), mucus (e.g. Muc 2). Moreover, some caecal microbial metabolites (including SCFAs, bile salts) were affected by NPL exposure. Based on these first results under physiological conditions, a study is currently carried out under a Western stress diet to mimic at-risk overweight populations (gut barrier defects, dysbiotic gut microbiota). Also see: https://micro2024.sciencesconf.org/558950/document