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Harnessing the Plastic-degrading Potential of Pseudomonas Species for Environmental Sustainability

IOP Conference Series Earth and Environmental Science 2024 2 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Azza Al Balushi, Yosra Amara, Khadija Al Balushi, Saif Eddine Mannai, Rim ben Saber, Ahmed Saleheddine Masmoudi, Ameur Cherif, Yasmine Souissi

Summary

This review examines Pseudomonas bacteria and their enzymes as candidates for breaking down plastics in the environment, noting that while these bacteria show promise, the catalytic efficiency of known plastic-degrading enzymes is too low and the mechanisms too poorly understood to achieve complete degradation. Incomplete biodegradation is itself a concern because partial breakdown of plastics generates microplastic fragments rather than eliminating them.

Abstract The prevalent utilization of synthetic plastics in contemporary society has made them indispensable. Nonetheless, the enduring predicament of plastic waste presents challenges to environmental sustainability and effective waste management. Plastic pollution can disrupt habitats and natural processes, diminishing the ecosystem’s resilience to climate change and directly impacting the livelihoods, food production capabilities, and social well-being of people. Moreover, plastic recycling itself can rise the production of polluting microplastic that find their way into water sources or air. In to address the environmental predicaments associated with plastics, it is imperative to examine the intricate interplay between microbes and polymers. Understanding this dynamic relationship is important as we strive to develop effective solutions and mitigate the adverse effects of plastic on our environment. Bacteria evolved strategies to survive and degrade plastics in contaminated environments. In this study, we focused on the ability of Pseudomonas species to degrade different sets of synthetic plastics including Low-Density polyethylene (LDPE), High-density polyethylene (HDPE), polyvinyl and polystyrene. Then a simulation study using kinetic models was conducted to determine optimum parameters for degradation ability. Our results indicate four different Pseudomonas strains P. aeruginosa strains , P. boreopolis and P. dehiensis two strains of which were able to grow in minimum media containing the different plastics as the only carbon sources reflecting their capacities to degrade these complexes polymers. Kinetic modeling for microbial growth of P. aeruginosa using batch and feed batch demonstrated that the feed batch fermentation procedure has the potential to produce Pseudomonas biomass on a larger scale, with a production of 250.7481 g/l after 72 hours. These results enhance their applicability in industrial settings particularly in bioremediation field. These findings highlight the important uses of bacterial strain of Pseudomonas as a promising strategy to depolymerize complex plastics into monomers for recycling or mineralize them into new biodegradable biomass.

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