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Enhanced degradation of microplastics by laccase under ambient conditions: Analysis of underlying molecular mechanisms

Journal of Hazardous Materials 2026
Rui Ding, Rui Ding, Linhan Xu, Linhan Xu, Xiaoling Shan, Xiaoling Shan, Xinran Qiu, Ling Ding, Ling Ding, Bin Zhang, Bin Zhang, Xujun Liang, Xujun Liang, Lingyan Zhu, Lingyan Zhu, Xuetao Guo

Summary

This study demonstrated that the enzyme laccase can degrade three types of microplastics — polyethylene (PE), PET, and PLA — by breaking apart polymer chains and transforming surface chemical groups, with biodegradable PLA showing the highest degradation efficiency. The mechanistic insights into how reactive oxygen species and electron transfer drive enzymatic degradation provide a foundation for developing enzyme-based treatments to remove microplastics from water and soil.

Microplastics (MPs) present a significant environmental challenge due to their widespread contamination and inherent resistance to biodegradation. Enzyme-driven biodegradation has emerged as a promising and environmentally friendly approach to mitigate microplastic pollution, yet the underlying molecular mechanisms remain poorly understood. This study systematically explored the laccase-mediated degradation of aliphatic (polyethylene, PE), aromatic (polyethylene terephthalate, PET), and biodegradable (polylactic acid, PLA) MPs, aiming to uncover the molecular-level mechanisms driving these processes. Our results demonstrated that laccase selectively degraded these MPs into low-molecular-weight products through polymer chain scission and functional group transformation. Notably, PLA exhibited the highest degradation efficiency, evidenced by significant surface modifications and molecular weight reduction. Reactive oxygen species (ROS) generation and electron-transfer processes were found to facilitate polymer chain cleavage and functional group evolution. Molecular dynamics simulations revealed distinct binding modes of laccase with different MPs: hydrophobic interactions dominated with PE, while hydrogen bonding and electrostatic forces played crucial roles with PET and PLA. These findings provide molecular-level insights into laccase-mediated MP degradation and offer valuable guidance for developing sustainable enzymatic strategies to tackle plastic pollution.

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