Adverse effects of environmentally relevant microplastics on in vivo endpoints, oxidative stress, and mitogen-activated protein kinase signaling pathway and multixenobiotic resistance system in the marine rotifer Brachionus plicatilis

This study compared the toxicological effects of environmentally relevant microplastics (MPs) on the marine rotifer Brachionus plicatilis, focusing on MPs derived from various sources, including fossil fuel-based low-density polyethylene, bio-based polylactic acid (PLA), biodegradable poly(butylene adipate-co-terephthalate), and a novel PLA modified with β-cyclodextrin. We assessed in vivo effects such as reproductive output and mortality, alongside in vitro oxidative stress responses, including oxidative stress, antioxidant enzyme activities, and activation of the mitogen-activated protein kinase (MAPK) signaling pathway and the multixenobiotic resistance (MXR) system. Reproductive output and lifespan reduced significantly across all MP types, ranging from 0.5 to 10 mg L-1, indicating compromised reproductive fitness and life maintenance. At an environmentally relevant concentration of 0.5 mg L-1, in vitro assessments revealed differential modulation of reactive oxygen species levels and antioxidant enzyme activities, contingent upon the specific MP type. Moreover, MAPK signaling pathway and MXR assays showed changes in phosphorylation and detoxification proteins depending on the type of MPs. This study highlights the ecological risks that various MPs, including bio-based, biodegradable, and petrochemical-based MPs, could pose in marine environments.