Isolation of culturable bacteria from gut of Zophobasmorio fed with polystyrene

Styrofoam, a widely utilized plastic, presents substantial environmental concerns due to its resistance to recycling and inherent non-biodegradability. Its persistence in terrestrial and aquatic ecosystems contributes significantly to plastic pollution, with accumulation in landfills and marine habitats becoming a pressing ecological issue. The production and degradation of Styrofoam can release hazardous chemicals, including styrene, which is associated with multiple health risks. Additionally, its fragmentation into microplastics poses severe threats to wildlife and disrupts ecological balance.Biodegradation of plastics through microbial action has emerged as a promising strategy for mitigating plastic pollution. In this study, we focused on isolating and characterizing bacterial strains capable of mineralizing Styrofoam. Using the larval stage of Zophobasmorio (superworms or giant mealworms) as a biological model, six cultivable bacterial isolates were obtained from Styrofoam-fed larvae. Detailed feeding trials were conducted to evaluate the behavioral responses and dietary preferences of superworms subjected to control, vegetable-based, and Styrofoam diets. The study provides insights into the potential of gut-associated microbial communities in facilitating Styrofoam degradation and offers a foundation for developing bioremediation strategies for plastic waste management.