0
Article ? AI-assigned paper type based on the abstract. Classification may not be perfect — flag errors using the feedback button. Tier 2 ? Original research — experimental, observational, or case-control study. Direct primary evidence. Marine & Wildlife Nanoplastics Sign in to save

Phenotypic profiling reveals polystyrene nanoplastics elicit sublethal and lethal effects on cellular morphology in rainbow trout gill epithelial cells

Environmental Science Nano 2025 3 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 48 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Lisbeth Díaz, Rebecca Klaper

Summary

Image-based phenotypic profiling revealed that polystyrene nanoplastics induced both sublethal and lethal morphological changes in rainbow trout gill epithelial cells at environmentally relevant concentrations, with dose-dependent effects on cell shape, nuclear integrity, and cytoskeletal organization.

Polymers

This work uses image-based profiling techniques to assess the environmental impacts of nanoplastics in fish gill cells at sublethal concentrations.

Sign in to start a discussion.

More Papers Like This

Article Tier 2

Polystyrene Nanoplastics Induce Multi-Organ Toxicity in the Rainbow Trout (Oncorhynchus mykiss): An Integrated Assessment of Physiological, Immunological, and Molecular Responses

Rainbow trout were exposed to polystyrene nanoplastics at three concentrations for 28 days and assessed for physiological, immunological, and molecular responses across multiple organs. NPs accumulated in liver, spleen, and intestine, causing dose-dependent oxidative stress, immune dysregulation, and altered gene expression, demonstrating multi-organ toxicity in a commercially important fish species.

Article Tier 2

Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models

Researchers tested the effects of two sizes of polystyrene nanoplastics on fish cell lines from both freshwater and marine species. They found that smaller 20-nanometer particles were significantly more toxic than larger 80-nanometer ones, causing cell death through apoptosis and disrupting multiple biological pathways. The study provides evidence that nanoplastic size is a key factor in determining toxicity to aquatic organisms.

Article Tier 2

Impact of sub-chronic polystyrene nanoplastics exposure on hematology, histology, and endoplasmic reticulum stress-related protein expression in Nile tilapia (Oreochromis niloticus)

Researchers exposed Nile tilapia to polystyrene nanoplastics for an extended period and found the particles caused blood cell changes, tissue damage in the liver and gills, and activated stress responses in cellular structures called the endoplasmic reticulum. Even the lowest concentration tested, which matches levels found in the environment, triggered harmful effects. Since tilapia is one of the most consumed farmed fish worldwide, these results highlight potential food safety concerns from nanoplastic contamination in aquaculture.

Article Tier 2

Polystyrene nanoplastics target lysosomes and affect lipid metabolism in RTgutGC and head kidney macrophages from Oncorhynchus mykiss

Researchers investigated the subcellular targets of polystyrene nanoplastics in rainbow trout intestinal cells and head kidney macrophages, finding that PS-NPs co-localized with lysosomes but not mitochondria and did not trigger reactive oxygen species production or alter oxidative metabolism. RNASeq analysis further revealed effects on lipid metabolism pathways, indicating that lysosomal targeting and lipid disruption are key mechanisms of nanoplastic toxicity in fish cells.

Article Tier 2

Bioaccumulation and homeostatic alterations in trout exposed to a sublethal dose of polystyrene nanoplastics

Researchers orally exposed rainbow trout to polystyrene nanoplastics and found the particles accumulated mainly in the gut and blood — not the liver — causing subtle immune and metabolic changes without visible tissue damage after 96 hours. These findings suggest nanoplastics selectively distribute in fish tissues and trigger mild biological responses even at sublethal doses.

Share this paper