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Impact of polystyrene microplastics on human alternative DNA structures
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Researchers investigated how polystyrene microplastics affect alternative DNA structures including G-quadruplexes and i-motifs, finding that PS microplastics induced conformational changes in these regulatory DNA structures, suggesting a potential pathway for genotoxic effects.
The widespread contamination of the environment by microplastics (MPs), particularly polystyrene (PS) and polyethylene (PE), poses significant ecological and health concerns. While much attention has been given to the physical impact of MPs, their molecular interactions, particularly with genetic material, remain largely unexplored. This study examines how PS and PE MPs influence the structure and stability of human alternative i-motif (iM) DNA, which plays a key role in regulating gene expression and maintaining genomic integrity. Using biophysical techniques such as UV-Vis spectroscopy, circular dichroism (CD), FT-IR spectroscopy, and thermal melting analysis, our results have demonstrated the effects of PS-MPs and PE-MPs of different sizes on HRAS2 iM DNA. Our results reveal that PS-MPs, particularly those with a 100 nm diameter, strongly interact with HRAS2 iM DNA, causing hypochromism and shifts in the UV-Vis spectra, along with changes in secondary structure observed by CD. FT-IR spectroscopy confirmed alterations in DNA conformation, and thermal melting data showed increased stability of the HRAS2 iM DNA in the presence of PS-MPs. In contrast, PE-MPs exhibited weaker interactions, likely due to differences in size and surface properties. These findings suggest that PS-MPs, due to their small size, may disrupt the structural integrity of iM DNA, potentially affecting gene regulation. This work highlights the need to consider both the physical and biochemical properties of MPs in assessing their environmental and health risks.
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Researchers investigated how polystyrene and polyethylene microplastics interact with alternative DNA structures called i-motifs that help regulate gene expression. The study found that 100-nanometer polystyrene particles strongly interacted with the HRAS2 i-motif DNA, altering its structure and increasing its thermal stability. These findings suggest that small microplastics may disrupt DNA regulatory structures at the molecular level, potentially affecting gene regulation.
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