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Unraveling the toxic trio: Combined effects of thifluzamide, enrofloxacin, and microplastics on Mytilus coruscus

Journal of Hazardous Materials 2025 1 citation ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Zeming Zhang, Haibo Liu, Huijuan Zuo, Yinan Wang, Aili Sun, Jiong Chen, Xizhi Shi

Summary

Researchers examined the combined effects of the pesticide thifluzamide, the antibiotic enrofloxacin, and polystyrene microplastics on mussels over four weeks. They found that co-exposure increased the accumulation of both chemicals in mussel tissue and worsened oxidative damage, neurotoxicity, and metabolic disruption compared to single exposures. The study suggests that the presence of microplastics in waterways can amplify the harmful effects of pesticides and antibiotics on edible shellfish.

Polymers
Body Systems

The presence of pesticides, antibiotics, and microplastics in aquatic environments poses a significant threat because of their persistence and potential harm to aquatic life and human health. However, few studies have explored their combined effects on bioaccumulation and toxicity in edible bivalves. This study examined the bioaccumulation and toxicological impacts of thifluzamide (TF) and enrofloxacin (ENR) on oxidative stress, neurotoxicity, detoxification, and metabolism in Mytilus coruscus after 4 weeks of exposure at the environmental level. The findings indicated that coexposure to TF and ENR or the presence of microplastic polystyrene (PS) increased TF and ENR accumulation in mussels and caused oxidative damage, as evidenced by elevated catalase and glutathione transferase activities and increased malondialdehyde (MDA) levels. Notably, compared with single exposures, coexposure to PS+TF, PS+ENR, or TF+ENR generally increased the MDA content, reduced acetylcholinesterase activity, and increased detoxification gene expression. Metabolomic analysis revealed that TF, ENR, and PS, either alone or combined, significantly disrupted multiple metabolic pathways by altering levels of glycerophospholipids, eicosanoids, amino acids, and nucleotides. Coexposure particularly worsened glycerophospholipid and arachidonic acid metabolism disturbances. These results suggest that combined exposure to TF, ENR or PS exacerbated the ecotoxicological effects of TF and ENR on M. coruscus. Taken together, the results of the present study could enhance our understanding of the environmental effects resulting from multipollutant interactions and their potential risks to seafood security.

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