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Bioremediation of PET-Contaminated Wastewater Using Shrimp Waste Activated Sludge: A Combined In Silico–Experimental Study

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Researchers used bacteria from shrimp waste sludge to break down PET plastic pollution in wastewater in lab tests. Computer modeling showed the bacteria's enzyme bound strongly to PET, and lab experiments found treated wastewater had up to 95% less solid waste and pollution markers within 10 hours, with a breakdown byproduct detected.

Polymers
Study Type Environmental

Polyethylene terephthalate (PET) plastic pollution is a long-standing environmental problem because it is highly resistant to natural degradation. This study investigates a poorly studied low-cost biocatalytic approach for the degradation of PET-derived wastewater using esterase-producing bacteria isolated from activated sludge of shrimp waste. Theoretical binding affinity and degradation performance were evaluated by the dual-approach strategy of molecular docking simulation (MOE software) and laboratory-scale degradation experiments. The docking analysis revealed that PET had a strong and stable binding interaction with the esterase enzyme, suggesting its catalytic ability. Shrimp waste activated sludge treatment experimentally resulted in reduction of Total Suspended Solids (TSS), Chemical Oxygen Demand (COD) and Biological Oxygen Demand (BOD) up to 95% within 10 hours. The degradation product of diethylene glycol (DEG) was confirmed by gas chromatography. These studies demonstrate a promising pathway towards an eco-friendly PET wastewater treatment based on waste valorization.

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