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Transcriptome sequencing dataset of the Pardosa pseudoannulata under cadmium and polystyrene microplastic stress
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Scientists studied how wolf spiders respond, at the genetic level, to cadmium (a toxic metal) and microplastic pollution by sequencing their gene activity. This paper itself doesn't report health conclusions—it's a dataset release documenting the raw genetic data—but it lays the groundwork for future research into how pollutants like microplastics disrupt biology in living creatures. Since spiders are predators that eat other insects, understanding how they're affected by these pollutants can help reveal how contamination moves through the food chain and potentially back to humans.
Raw data sequences of the wolf spider (Pardosa pseudoannulata). Total RNA extraction was performed with TRNzol Universal Reagent (TIANGEN Biotech, Beijing, China) according to the manufacturer’s instructions. Sequencing libraries were constructed using the Fast RNA-seq Lib Prep Kit V2 (Cat. No. Rk20306, ABclonal, Wuhan, China). High-throughput sequencing was conducted for all samples on an Illumina NovaSeq X Plus instrument. Raw sequencing reads were processed to obtain clean reads by removing adapter sequences, reads containing poly-N regions, and low-quality reads. Clean reads were then assembled de novo using Trinity to generate reference transcripts for downstream analysis. The data files are in FASTQ format.
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