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Tier 2
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Original research — experimental, observational, or case-control study. Direct primary evidence.
Detection Methods
Food & Water
Human Health Effects
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Protocol for a Small-scale Algal Toxicity Assay for Testing the Acute Toxicity of Plastic Leachates v2
2025
Gillian Champoir
Summary
Version 2 of a protocol for a small-scale algal toxicity assay using glass tubes and fluorescence measurements to test the acute toxicity of plastic leachates, with updated procedural details for improved reproducibility.
This protocol uses glass culture tubes as opposed to a well plate to avoid potential plastic contamination. The assay runs for two weeks or longer if so desired. Instead of using cell counts, fluorescence is used as a proxy for growth rate, as it can be calculated into µ upon completion of the experiment.