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Protocol for a Small-scale Algal Toxicity Assay for Testing the Acute Toxicity of Plastic Leachates v1
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This protocol describes a small-scale algal toxicity assay using glass culture tubes (to avoid plastic contamination) and fluorescence-based growth measurement to assess the acute toxicity of plastic leachates on algae over two weeks.
This protocol uses glass culture tubes as opposed to a well plate to avoid potential plastic contamination. The assay runs for two weeks or longer if so desired. Instead of using cell counts, fluorescence is used as a proxy for growth rate, as it can be calculated into µ upon completion of the experiment.
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Protocol for a Small-scale Algal Toxicity Assay for Testing the Acute Toxicity of Plastic Leachates v2
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Version 2 of a protocol for a small-scale algal toxicity assay using glass tubes and fluorescence measurements to test the acute toxicity of plastic leachates, with updated procedural details for improved reproducibility.
Effects of Microplastic Leachates on Phytoplankton : A Laboratory Study on Nodularia spumigena and Phaeodactylum tricornutum
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This thesis investigated the effects of chemical leachates from microplastics on two phytoplankton species, a cyanobacterium and a diatom, in controlled laboratory experiments. Researchers measured growth and physiological responses of the microalgae when exposed to water extracts from plastic materials. The study contributes to understanding the indirect toxicity of microplastics to primary producers through dissolved chemical release.
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A new ecotoxicological assessment approach for plastic leachates derived from alternating UV and hydrolysis weathering found that polystyrene leachates produced measurable algae growth inhibition and that oxidized polymer degradation products may be more bioavailable and toxic than pristine particles.
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Researchers studied the effects of chemical leachates from UV-weathered microplastics on the green algae Scenedesmus vacuolatus. The study found that leachates from additive-containing electronic waste plastics caused severe effects, while virgin polymer leachates generally did not, except for polyethylene, whose degradation products reduced algae growth and photosynthetic activity.
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