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Tier 2
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Original research — experimental, observational, or case-control study. Direct primary evidence.
Detection Methods
Food & Water
Human Health Effects
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Protocol for a Small-scale Algal Toxicity Assay for Testing the Acute Toxicity of Plastic Leachates v1
2025
Gillian Champoir
Summary
This protocol describes a small-scale algal toxicity assay using glass culture tubes (to avoid plastic contamination) and fluorescence-based growth measurement to assess the acute toxicity of plastic leachates on algae over two weeks.
This protocol uses glass culture tubes as opposed to a well plate to avoid potential plastic contamination. The assay runs for two weeks or longer if so desired. Instead of using cell counts, fluorescence is used as a proxy for growth rate, as it can be calculated into µ upon completion of the experiment.