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Estudio de las interacciones entre los miembros de un consorcio bacteriano e implicaciones de las mismas en la biodegradación de plásticos

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Bacterial consortium (Pseudomonas + Bacillus) degrades LLDPE far more effectively than pure cultures, especially with glucose as cometabolic substrate.

Polymers

El aumento en la producción de plástico y la acumulación de sus residuos en los ecosistemas supone un grave problema a nivel global. Los métodos físico-químicos que se emplean actualmente para el tratamiento de los residuos plásticos resultan ineficientes por lo que es necesaria la búsqueda de alternativas sostenibles para acabar con este tipo de contaminación. Un enfoque biotecnológico para solucionar este problema es el empleo de consorcios microbianos, cuya eficiencia en la biodegradación de contaminantes es mayor que el de sus cultivos puros. En este trabajo, se pretenden caracterizar las relaciones existentes entre los dos miembros, Pseudomonas alloputida REBP7 y Bacillus subtilis RBM2, de un consorcio microbiano capaz de biodegradar polietileno lineal de baja densidad (LLDPE) según estudios previos. También se pretende encontrar cual es el medio de cultivo óptimo para la degradación de este polímero plástico. Para ello se realizaron diversos ensayos para comprobar las posibles interacciones positivas y negativas entre los dos microorganismos del consorcio. Se realizó una comparativa entre la eficiencia en la degradación de plástico entre el consorcio y los cultivos puros de estos dos microorganismos en medios de cultivo con LLDPE como única fuente carbonada y con distintos sustratos cometabólicos, para comprobar su influencia en la degradación del LLDPE. Los resultados mostraron que, aunque existían ciertas interacciones negativas entre los miembros, estas no eran relevantes para el desarrollo del consorcio. Estos resultados se verificaron cuando se comprobó que el consorcio mostraba una capacidad de degradación del LLDPE muchísimo mayor que los cultivos puros, sobre todo en aquellos medios de cultivo que incorporaban como sustrato cometabólico tan solo glucosa. The increase in plastic production and the accumulation of this waste in ecosystems is a serious global problem. The physical-chemical methods currently used to treat this plastic waste are inefficient, so it is necessary to search for sustainable alternatives to end this type of pollution. A biotechnological approach to solve this problem is the use of microbial consortia, whose efficiency in the biodegradation of pollutants is higher than the use of pure cultures. In this work, we intend to characterize the relationships between the two members, seudomonas alloputida REBP7 and Bacillus subtilis RBM2, of a microbial consortium capable of biodegrading linear low density polyethylene (LLDPE) according to previous studies. It is also intended to find the optimal culture medium for the degradation of this plastic polymer. For this purpose, several tests were carried out to check the possible positive and negative interactions between the two microorganisms in the consortium. A comparison between the efficiency in plastic degradation between the consortium as pure cultures of these two microorganisms in culture media with LLDPE as the only carbon source and with different cometabolic substrates was carried out to check their influence on the degradation of LLDPE. The results showed that, although there were some negative interactions between the members, these were not relevant for the development of the consortium. These results were verified when it was found that the consortium showed a much higher LLDPE degradation capacity than pure cultures, especially in those culture media that incorporated only glucose as a cometabolic substrate.

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