Papers

61,005 results
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Article Tier 2

Uptake and Accumulation of Polystyrene Microplastics in Zebrafish (Danio rerio) and Toxic Effects in Liver

Researchers exposed zebrafish to polystyrene microplastics of two different sizes and tracked where the particles accumulated in the body. They found that smaller particles (5 micrometers) built up in the gills, liver, and gut, while larger particles (20 micrometers) mainly stayed in the gills and gut. The microplastics caused liver inflammation, oxidative stress, and disrupted fat metabolism, suggesting that ingested microplastics can damage internal organs in fish.

2016 Environmental Science & Technology 1980 citations
Article Tier 2

Exposure to microplastics decreases swimming competence in larval zebrafish (Danio rerio)

Researchers exposed zebrafish embryos and larvae to polystyrene microplastics and found that the particles adhered to egg surfaces before being ingested into the stomach and intestines after hatching. At higher concentrations, exposed larvae showed significantly reduced swimming distance and speed, along with upregulated genes related to inflammation and oxidative stress. The study suggests that microplastic exposure can impair swimming ability in larval fish, which could have broader ecological consequences for population fitness.

2019 Ecotoxicology and Environmental Safety 201 citations
Article Tier 2

Occurrence and characterization of microplastic content in the digestive system of riverine fishes

Researchers found microplastics in 93.8% of riverine fish examined, with polystyrene, polyethylene, and nylon being the most common polymer types concentrated near urban and industrial areas, and small particles (0.025-1 mm) predominating across species.

2021 Journal of Environmental Management 32 citations
Article Tier 2

Oxidative damage and decreased aerobic energy production due to ingestion of polyethylene microplastics by Chironomus riparius (Diptera) larvae

Researchers exposed Chironomus riparius larvae to three size classes of polyethylene microplastics and found that all sizes were ingested, causing oxidative damage and reduced aerobic energy production, with the smallest particles causing the greatest harm.

2020 Journal of Hazardous Materials 102 citations
Article Tier 2

Microplastic particles cause intestinal damage and other adverse effects in zebrafish Danio rerio and nematode Caenorhabditis elegans

Researchers exposed zebrafish and nematodes to five common types of microplastics and found that several types caused intestinal damage, including cracking of the gut lining. In nematodes, microplastics significantly reduced survival, body length, and reproduction, with 1-micrometer particles causing the most severe effects. The findings suggest that intestinal damage and oxidative stress are primary mechanisms through which microplastics harm aquatic organisms.

2017 The Science of The Total Environment 1334 citations
Article Tier 2

Study of the effects of nanoplastics ingestion in a freshwater fish ( Danio rerio )

Researchers exposed zebrafish to polystyrene nanoplastics and found evidence of intestinal damage, oxidative stress, and behavioral changes. The study adds to growing evidence that nanoplastics in freshwater environments can harm fish health, with potential implications for the health of ecosystems and fish-eating humans.

2021 Annals of Medicine 4 citations
Article Tier 2

Effects of microplastics in freshwater fishes health and the implications for human health

This review examines how microplastics affect the health of freshwater fish, which are a major protein source for billions of people. Fish ingest microplastics that accumulate in their guts, gills, and tissues, leading to inflammation, oxidative stress, and disrupted growth. Since microplastics in fish tissue can transfer to humans through the food chain, this is relevant to both ecosystem and human health.

2023 Brazilian Journal of Biology 39 citations
Article Tier 2

Biomarker responses in zebrafish (Danio rerio) larvae exposed to pristine low-density polyethylene fragments

Researchers exposed zebrafish larvae to pristine low-density polyethylene microplastics and measured biomarker responses, finding altered oxidative stress and metabolic indicators even in early developmental stages.

2017 Environmental Pollution 153 citations
Article Tier 2

Dietary Microplastic Administration during Zebrafish (Danio rerio) Development: A Comprehensive and Comparative Study between Larval and Juvenile Stages

Researchers compared how zebrafish at different developmental stages respond to microplastics mixed into their feed, testing various particle sizes and concentrations. They found that larval fish were more sensitive than juveniles, and that smaller microplastics caused greater accumulation in the body. The study provides evidence that early life stages of fish are particularly vulnerable to dietary microplastic exposure, which is relevant for understanding contamination risks in aquaculture.

2023 Animals 15 citations
Article Tier 2

Microplastics in freshwater fishes: Occurrence, impacts and future perspectives

This review synthesizes current knowledge about microplastic contamination in freshwater fish, which serve as important indicators of plastic pollution in rivers and lakes. Researchers found that microplastic ingestion patterns in fish are related to body size, feeding habits, and local urbanization levels, with controlled studies showing various effects on fish physiology and behavior. While fish can typically expel most microplastics quickly, certain particle shapes and sizes may remain in the body or cross into other organs through the intestinal wall.

2021 Fish and Fisheries 149 citations
Article Tier 2

Acute toxic effects of polyethylene microplastic on adult zebrafish

Researchers exposed adult zebrafish to polyethylene microplastics of various sizes to identify physical effects, behavioral changes, and gene expression impacts. They found that microplastic ingestion varied by particle size and that exposure altered expression of detoxification and reproduction-related genes. The study suggests that microplastic pollution at environmentally relevant concentrations could affect both the health and reproductive capacity of fish.

2019 Ecotoxicology and Environmental Safety 290 citations
Article Tier 2

Environmental microplastics disrupt swimming activity in acute exposure in Danio rerio larvae and reduce growth and reproduction success in chronic exposure in D. rerio and Oryzias melastigma

Researchers exposed zebrafish and marine medaka to environmental microplastics collected from Caribbean beaches and found that acute exposure disrupted swimming behavior in larvae, while chronic dietary exposure reduced growth and reproduction by up to 70%. The study suggests that environmentally relevant microplastic concentrations can cause cross-generational effects, with offspring of exposed fish showing premature mortality.

2022 Environmental Pollution 72 citations
Article Tier 2

Polystyrene nano/microplastics induce microbiota dysbiosis, oxidative damage, and innate immune disruption in zebrafish

Researchers exposed zebrafish to polystyrene particles of two different sizes and found that both nano- and micro-sized plastics disrupted gut bacteria, caused oxidative damage, and altered immune responses. The severity of effects depended on particle size and concentration, with smaller particles and higher doses causing more harm. The study suggests that plastic particles in waterways may pose a broader threat to fish health than previously understood, affecting digestion, stress defenses, and immunity simultaneously.

2022 Microbial Pathogenesis 86 citations
Article Tier 2

Differential developmental and proinflammatory responses of zebrafish embryo to repetitive exposure of biodigested polyamide and polystyrene microplastics

Researchers found that polyamide and polystyrene microplastics undergo very different changes when exposed to digestive fluids, with polyamide breaking down significantly while polystyrene remains largely intact. When zebrafish embryos were repeatedly exposed to these digested microplastics, both types caused developmental problems and inflammation, but through different biological mechanisms. The study suggests that how microplastics are transformed during digestion may be just as important as the original particle when assessing health risks.

2023 Journal of Hazardous Materials 19 citations
Article Tier 2

Assessing the size-dependent effects of microplastics on zebrafish larvae through fish lateral line system and gut damage

Researchers exposed zebrafish larvae to high-density polyethylene microplastics in three size categories and assessed their effects on the fish sensory system and gut. They found that larger particles caused the most damage to the lateral line sensory system, while particles smaller than 100 micrometers were ingested and caused gastrointestinal tract changes. All sizes of microplastics altered larval swimming behavior, with distinct patterns depending on particle size.

2022 Marine Pollution Bulletin 45 citations
Article Tier 2

Microplastics in Limnic Ecosystems - Investigation of Biological Fate and Effects of Microplastic Particles and Associated Contaminants in Zebrafish (Danio rerio)-

This doctoral thesis investigated how microplastics and their associated chemical contaminants affect zebrafish (Danio rerio) in freshwater environments, including ingestion, tissue accumulation, and toxicological effects. The research demonstrates that microplastics can act as vectors for pollutants like pesticides and pharmaceuticals, compounding their harmful effects on aquatic organisms.

2020 heiDOK (Heidelberg University) 5 citations
Article Tier 2

[Effects of Microplastics on Embryo Hatching and Intestinal Accumulation in Larval Zebrafish Danio rerio].

Researchers examined the effects of two sizes of polystyrene microplastics (10 µm and 0.5 µm) on embryo hatching rates and intestinal accumulation in fish larvae, finding that the smaller submicron particles accumulated more readily in intestinal tissue, raising concerns about early developmental exposure.

2021 PubMed 7 citations
Article Tier 2

An end to the controversy over the microscopic detection and effects of pristine microplastics in fish organs

Researchers conducted feeding experiments with zebrafish to resolve conflicting findings about microplastic detection and effects in fish. They found that zebrafish generally recognize plastic particles as inedible but ingest them when mixed with food, and effectively eliminate most particles within 24 hours, though larger particles take longer to clear. The study demonstrates using confocal microscopy to track plastic microbead uptake and translocation in fish for the first time, while finding no histopathological damage from pristine microplastic ingestion.

2020 Scientific Reports 141 citations
Article Tier 2

Environmental microplastics accumulate in gonads in a sex-dependent manner and alter reproductive success in zebrafish (Danio rerio)

Zebrafish exposed to environmentally sourced microplastics accumulated particles preferentially in the testes rather than ovaries, and males showed the highest gonadal microplastic loads along with PCB co-contaminant effects including inhibited apoptosis and hepatotoxicity.

2025 Journal of Hazardous Materials Advances
Article Tier 2

Deleterious Effects of Polypropylene Microplastic Ingestion in Nile Tilapia (Oreochromis niloticus)

Researchers fed Nile tilapia daily doses of polypropylene microplastics for 30 days and observed significant health effects including changes in blood cell counts, altered gut bacteria, and tissue damage to the intestines and liver. The higher dose group showed more pronounced effects, including elevated inflammatory markers and signs of oxidative stress. The study provides evidence that chronic ingestion of microplastics commonly found in aquatic environments can cause meaningful harm to a widely consumed fish species.

2023 Bulletin of Environmental Contamination and Toxicology 19 citations
Article Tier 2

Microplastics pollution in freshwater fishes in the South of Italy: Characterization, distribution, and correlation with environmental pollutants

Researchers investigated the presence, abundance, and polymer composition of microplastics in the gastrointestinal tracts of freshwater fish from rivers in southern Italy, and evaluated correlations between microplastic ingestion and environmental pollutant levels. They found microplastics in multiple fish species, with fiber morphologies predominating, and identified associations between microplastic burden and co-occurring chemical contaminants in the sampled environments.

2022 The Science of The Total Environment 19 citations
Article Tier 2

Effects of polystyrene nano- and microplastics and of microplastics with sorbed polycyclic aromatic hydrocarbons in adult zebrafish

Researchers exposed adult zebrafish to nano- and microplastic particles of different sizes, some carrying additional chemical pollutants, over a 21-day period. The study found that nanoplastics and microplastics triggered different stress responses in the fish, with nanoplastics altering antioxidant gene activity and microplastics causing liver changes. The findings suggest that particle size matters when it comes to the biological effects of plastic pollution in aquatic organisms.

2024 The Science of The Total Environment 25 citations
Article Tier 2

Bioaccumulation of various nanoplastic particles in larval zebrafish (Danio rerio)

Researchers exposed larval zebrafish (Danio rerio) to 40-60 nm and 100 nm polystyrene nanoplastic particles using standard fish embryo toxicity and general behavioral toxicity assays from 6-120 hours post-fertilization, combining toxicity endpoints with fluorescence microscopy to confirm particle uptake and excretion. The study demonstrated nanoplastic accumulation within zebrafish larvae at tested concentrations, providing mechanistic insights into aquatic organism exposure dynamics for nanoplastics.

2022 Zenodo (CERN European Organization for Nuclear Research)
Article Tier 2

Rethinking the relevance of microplastics as vector for anthropogenic contaminants: Adsorption of toxicants to microplastics during exposure in a highly polluted stream - Analytical quantification and assessment of toxic effects in zebrafish (Danio rerio)

Researchers exposed microplastics in a highly polluted stream to assess their role as contaminant vectors, then tested effects on zebrafish, finding that naturally contaminated microplastics had limited additional toxicity compared to the polluted water itself.

2021 The Science of The Total Environment 26 citations